Hormonal regulation of expression of growth differentiation factor-9 receptor type I and II genes in the bovine ovarian follicle

Jayawardana, Barana C.; Shimizu, Takashi; Nishimoto, Hiromi; Kaneko, Etsushi; Tetsuka, Masafumi; Miyamoto, Akio

doi:10.1530/rep.1.00885

Cited by 47 publications

(39 citation statements)

References 45 publications

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“…However, combined treatment with FSH and oestradiol increased the expression of all investigated genes, i.e., BMPRII, BMPRIB and ALK-5. This observation supported data from previous studies suggesting the existence of paracrine activation pathways between oocytes and granulosa cells, and the regulation of BMP15 receptors by FSH and E 2 secretion (Jayawardana et al 2006;Chen et al 2009a). Furthermore, it is evident that hormones of the pituitary and ovary have a significant role in the regulation of granulosa cell differentiation.…”

Section: Tgf Gene Expression In Relation To Angiogenesis and Folliculsupporting

confidence: 90%

The role of TGF superfamily gene expression in the regulation of folliculogenesis and oogenesis in mammals: a review

Piotrowska¹,

Kempisty²,

Sosinska³

et al. 2013

Vet. Med.

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ABSTRACT:The normal differentiation of follicles from the preantral to the antral stage is regulated by the synthesis and secretion of several important growth factors. Moreover, the proper growth and development of the oocyte and its surrounding somatic granulosa-cumulus cells is accomplished through the activation of paracrine pathways that form a specific cross-talk between the gamete and somatic cells. It has been shown that several growth factors produced by the ovary are responsible for the proper growth and development of follicles. The developmental competence of mammalian oocytes (also termed developmental potency) is defined as the ability of female gametes to reach maturation (the MII stage) and achieve successful monospermic fertilisation. Proper oocyte development during folliculo-and oogenesis also plays a critical role in normal zygote and blastocyst formation, as well as implantation and the birth of healthy offspring. Several molecular markers have been used to determine the developmental potency both of oocytes and follicles. The most important markers include transforming growth factor beta superfamily genes (TGFB), and the genes in this family have been found to play a crucial role in oocyte differentiation during oogenesis and folliculogenesis. In the present review, we summarise several molecular aspects concerning the assessment of mammalian oocyte developmental competence. In addition, we present the molecular mechanisms which activate important growth factors within the TGFB superfamily that have been shown to regulate not only follicle development but also oocyte maturation.

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Section: Tgf Gene Expression In Relation To Angiogenesis and Folliculsupporting

confidence: 90%

The role of TGF superfamily gene expression in the regulation of folliculogenesis and oogenesis in mammals: a review

Piotrowska¹,

Kempisty²,

Sosinska³

et al. 2013

Vet. Med.

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“…However, a positive correlation was observed between mRNA expression of BMPR2 in PCOS cumulus cells and levels of testosterone and androstenedione in FF from pre-ovulatory follicles. In fact, several studies have shown that BMP receptors are hormonally regulated [22][23][24]. PCOS-related hyperandrogenism can exerts complex effects on the ovarian follicle.…”

Section: Discussionmentioning

confidence: 99%

“…Data from microarray studies indicate that androgen might play a role in up-regulate gene expression in PCOS oocytes [5] and androgen treatment in female rhesus monkeys increases mRNA expression of FSH and IGF-1 receptors in granulosa cells and follicle primordial oocytes [39]. In bovine granulosa cells, estrogen used alone and in combination with FSH has been shown to up-regulate BMPR2 mRNA expression [22,24]. Supporting this finding, a study using a human granulosa-like tumor cell line showed that FSH up-regulated BMPR2 in GCs [23].…”

Section: Discussionmentioning

confidence: 99%

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Single-cell expression analysis of BMP15 and GDF9 in mature oocytes and BMPR2 in cumulus cells of women with polycystic ovary syndrome undergoing controlled ovarian hyperstimulation

Resende

Vireque

Santana

et al. 2012

J Assist Reprod Genet

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Purpose To detect expression of bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) in oocytes, and their receptor type 2 receptor for BMPs (BMPR2) in cumulus cells in women with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization (IVF), and determine if BMPR2, BMP15, and GDF9 expression correlate with hyperandrogenism in FF of PCOS patients. Methods Prospective case-control study. Eighteen MIIoocytes and their respective cumulus cells were obtained from 18 patients with PCOS, and 48 MII-oocytes and cumulus cells (CCs) from 35 controls, both subjected to controlled ovarian hyperstimulation (COH), and follicular fluid (FF) was collected from small (10-14 mm) and large (>18 mm) follicles. RNeasy Micro Kit (Qiagen ® ) was used for RNA extraction and gene expression was quantified in each oocyte individually and in microdissected cumulus cells from cumulus-oocyte complexes retrieved from preovulatory follicles using qRT-PCR. Chemiluminescence and RIA assays were used for hormone assays. Results BMP15 and GDF9 expression per oocyte was higher among women with PCOS than the control group. A positive correlation was found between BMPR2 transcripts and hyperandrogenism in FF of PCOS patients. Progesterone values in FF were lower in the PCOS group. Conclusion We inferred that BMP15 and GDF9 transcript levels increase in mature PCOS oocytes after COH, and might inhibit the progesterone secretion by follicular cells in PCOS follicles, preventing premature luteinization in cumulus cells. BMPR2 expression in PCOS cumulus cells might be regulated by androgens.

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“…One possible way to achieve this effect could be down-regulation of TGFB1 action. Indeed, some studies provide evidence that FSH treatment decreases TGFB1 and TGFBR1 (activin receptor-like kinase 5) mRNA expressions in cultured bovine granulosa cells (Nilsson et al 2003, Jayawardana et al 2006. Further studies, including experiments involving manipulation of TGFB1 activity in vivo and in vitro, will be required explore these possibilities.…”

Section: Tgfb1 Regulation Of Steroidogenesis and Apoptosismentioning

confidence: 99%

Transforming growth factor-β1 inhibits luteinization and promotes apoptosis in bovine granulosa cells

Zheng¹,

Boerboom²,

Carrière³

2009

Reproduction

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We have previously shown that TGFB1 inhibits estradiol (E 2 ) and progesterone (P 4 ) biosynthesis in FSH-stimulated bovine granulosa cells by selective inhibition of steroidogenic enzymes. The objective of this study was to assess the effects of TGFB1 on E 2 and P 4 steroidogenesis in bovine granulosa cells cultured in the absence of FSH and to measure the effects of TGFB1 on cell proliferation and apoptosis in the presence and absence of FSH. Bovine granulosa cells from 2 to 5 mm follicles were cultured in serum-free medium for 2-6 days. In the absence of FSH, the secretion of P 4 increased with time in culture (P!0.05). Addition of TGFB1 for 6 days decreased P 4 secretion and mRNA levels of the P 4 synthesis-associated genes STAR, CYP11A1, HSD3B1, and GSTA (P!0.05). In the absence of FSH, the secretion of E 2 decreased and addition of TGFB1 for 6 days partially reversed this decline and stimulated E 2 biosynthesis, CYP19A1 and HSD17B1 mRNA levels and CYP19A1 activity (P!0.05). Conversely, TGFB1 did not affect HSD17B7 expression and HSD17B-reducing activity. TGFB1 decreased the proportion of cells in the G0/G1 and SCG2/M phases in FSHstimulated and unstimulated granulosa cells (P!0.05). Furthermore, in the presence or absence of FSH, TGFB1 increased the proportion of cells in apoptosis measured by propidium iodide staining and flow cytometry and confirmed by increased levels of cleaved caspase-3 (P!0.05). Our results therefore indicate that TGFB1 inhibits luteinization in cultured bovine granulosa cells while maintaining an estrogenic phenotype, and this effect was associated with increased apoptosis.

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Hormonal regulation of expression of growth differentiation factor-9 receptor type I and II genes in the bovine ovarian follicle

Cited by 47 publications

References 45 publications

The role of TGF superfamily gene expression in the regulation of folliculogenesis and oogenesis in mammals: a review

The role of TGF superfamily gene expression in the regulation of folliculogenesis and oogenesis in mammals: a review

Single-cell expression analysis of BMP15 and GDF9 in mature oocytes and BMPR2 in cumulus cells of women with polycystic ovary syndrome undergoing controlled ovarian hyperstimulation

Transforming growth factor-β1 inhibits luteinization and promotes apoptosis in bovine granulosa cells

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