Hormonal regulation of<i>mummy</i>is needed for apical extracellular matrix formation and epithelial morphogenesis in<i>Drosophila</i>

Tonning, Anna; Helms, Sigrun; Schwarz, Heinz; Uv, Anne; Moussian, Bernard

doi:10.1242/dev.02206

Cited by 77 publications

(68 citation statements)

References 53 publications

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“…One line, 2L0058, showed a convoluted dorsal trunk. We therefore tested it for complementation of mutations in known genes on the second chromosome that show this mutant phenotype ½convoluted, varicose, nervana2 (Beitel and Krasnow 2000), and mummy (cystic) (Araujo et al 2005;Tonning et al 2006) but found it not to be allelic to any of them. However, the observation that a line from phenotypic class A had a convoluted dorsal trunk suggested that other lines from this class might also have such a phenotype and be allelic to the genes mentioned above.…”

Section: Resultsmentioning

confidence: 99%

A Clonal Genetic Screen for Mutants Causing Defects in Larval Tracheal Morphogenesis in Drosophila

Baer¹,

Bilstein²,

Leptin

2007

Genetics

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The initial establishment of the tracheal network in the Drosophila embryo is beginning to be understood in great detail, both in its genetic control cascades and in its cell biological events. By contrast, the vast expansion of the system during larval growth, with its extensive ramification of preexisting tracheal branches, has been analyzed less well. The mutant phenotypes of many genes involved in this process are probably not easy to reveal, as these genes may be required for other functions at earlier developmental stages. We therefore conducted a screen for defects in individual clonal homozygous mutant cells in the tracheal network of heterozygous larvae using the mosaic analysis with a repressible cell marker (MARCM) system to generate marked, recombinant mitotic clones. We describe the identification of a set of mutants with distinct phenotypic effects. In particular we found a range of defects in terminal cells, including failure in lumen formation and reduced or extensive branching. Other mutations affect cell growth, cell shape, and cell migration.

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Section: Resultsmentioning

confidence: 99%

A Clonal Genetic Screen for Mutants Causing Defects in Larval Tracheal Morphogenesis in Drosophila

Baer¹,

Bilstein²,

Leptin

2007

Genetics

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“…Tube expansion and extension relies on a luminal chitin filament that assembles transiently in the tracheal tubes (reviewed in Swanson and Beitel, 2006). The metabolic pathway that leads to chitin synthesis involves several enzymes, among which are Mmy and Kkv (Araujo et al, 2005;Devine et al, 2005;Tonning et al, 2006;Tonning et al, 2005). In addition, other proteins are known to participate in the proper assembly and/or modification of the chitin filament, such as Knk, Rtv , Verm and Serp (Luschnig et al, 2006;Wang et al, 2006).…”

Section: Ttk In Tube Sizementioning

confidence: 99%

Tramtrack regulates different morphogenetic events duringDrosophilatracheal development

2007

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*Tramtrack (Ttk) is a widely expressed transcription factor, the function of which has been analysed in different adult and embryonic tissues in Drosophila. So far, the described roles of Ttk have been mainly related to cell fate specification, cell proliferation and cell cycle regulation. Using the tracheal system of Drosophila as a morphogenetic model, we have undertaken a detailed analysis of Ttk function. Ttk is autonomously and non-autonomously required during embryonic tracheal formation. Remarkably, besides a role in the specification of different tracheal cell identities, we have found that Ttk is directly involved and required for different cellular responses and morphogenetic events. In particular, Ttk appears to be a new positive regulator of tracheal cell intercalation. Analysis of this process in ttk mutants has unveiled cell shape changes as a key requirement for intercalation and has identified Ttk as a novel regulator of its progression. Moreover, we define Ttk as the first identified regulator of intracellular lumen formation and show that it is autonomously involved in the control of tracheal tube size by regulating septate junction activity and cuticle formation. In summary, the involvement of Ttk in different steps of tube morphogenesis identifies it as a key player in tracheal development.

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“…Glycosylation plays a variety of roles during development, including signal transduction, morphogen distribution, extracellular matrix (ECM) formation, and cell-cell and cell-ECM interactions (Haltiwanger 2002;Baeg et al 2004;Tonning et al 2006). For example, Fringe-dependent glycosylation modifies the affinity of Notch for various ligands differently.…”

Section: Introductionmentioning

confidence: 99%

Spatial and temporal regulation of glycosylation during Drosophila eye development

2009

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Glycosylation plays an essential role during development, in processes such as morphogen distribution, cell-to-cell communication, and extracellular matrix formation. Glycosylation is regulated during development in both a spatial and temporal manner. This study presents a detailed description of glycan distribution from late pupal to adult stages in Drosophila ommatidia by using nine different lectins. The lectins ConA, LCA, and DSA, which recognize high-mannose or complex types of N-linked glycans stain both apical and basolateral membranes of photoreceptor cells, whereas SBA, DBA, and ABA lectins, which bind to mucin-type O-glycans, label the inter-rhabdomeral space. The O-linked GlcNAc moiety is strongly labeled by WGA on the nuclear membrane. The localization of these glycans does not change throughout late pupal development. In contrast, the abundance of O-linked glycans, bisected oligosaccharides, and GlcNAc-containing glycans detected by PNA, PHA-E4, and WGA, respectively, is reduced in rhabdomeres and other subcellular domains during late pupal development. Some of these glycans have also been detected in the Golgi and/or putative secretory vesicles, suggesting their dynamic transport during development. These glycans, whose expression is dynamically regulated in a spatial and temporal manner, may therefore play critical roles in ommatidial development.

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Hormonal regulation ofmummyis needed for apical extracellular matrix formation and epithelial morphogenesis inDrosophila

Cited by 77 publications

References 53 publications

A Clonal Genetic Screen for Mutants Causing Defects in Larval Tracheal Morphogenesis in Drosophila

A Clonal Genetic Screen for Mutants Causing Defects in Larval Tracheal Morphogenesis in Drosophila

Tramtrack regulates different morphogenetic events duringDrosophilatracheal development

Spatial and temporal regulation of glycosylation during Drosophila eye development

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