Hormonal regulation of non-cystic fibrosis transmembrane conductance regulator ion channels in the endocervix

Roberts, Mackenzie; Yao, Shouguang; Wei, Shuhao; Jensen, Jeffrey T.; Han, Leo

doi:10.1016/j.xfss.2023.03.002

Cited by 2 publications

(2 citation statements)

References 42 publications

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“…We then produced a list of genes and gene classes associated with epithelial mucus production and secretion throughout the menstrual cycle (Table 1). [7][8][9][10][29][30][31] From this list we found 28 differentially expressed genes (DEGs) in estradiol-only conditions (EE vs CC) and 10 DEGs in progesterone-treated conditions (EP vs EE) (Figure 3), with multidimensional scaling illustrating alterations in gene expression among our samples and conditions (Figure 4). These genes are subdivided into classes based on their role in mucus synthesis and modification, consisting of mucins, ion channels, post-secretory mucus modifiers, mucus modifying enzymes and proteins, cilia-associated genes, cell signaling genes, and aquaporins.…”

Section: Resultsmentioning

confidence: 99%

Transcriptional profiling of mucus production and modification in rhesus macaque endocervical cells under hormonal regulation

Rapp,

Wei,

Roberts

et al. 2023

Preprint

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Objective: Endocervical mucus production is a key regulator of fertility throughout the menstrual cycle. With cycle-dependent variability in mucus quality and quantity, cervical mucus can either facilitate or block sperm ascension into the upper female reproductive tract. This study seeks to identify genes involved in the hormonal regulation of mucus production, modification, and regulation through profiling the transcriptome of endocervical cells from the non-human primate, the Rhesus Macaque (Macaca mulatta). Design: Experimental. Setting: Translational science laboratory. Intervention: We treated differentiated primary endocervical cultures with estradiol (E2) and progesterone (P4) to mimic peri-ovulatory and luteal-phase hormonal changes. Using RNA-sequencing, we identified differential expression of gene pathways and mucus producing and modifying genes in cells treated with E2 compared to hormone-free conditions and E2 compared to E2-primed cells treated with P4. Main Outcome Measures: We pursued differential gene expression analysis on RNA-sequenced cells. Sequence validation was done using qPCR. Results: Our study identified 158 genes that show significant differential expression in E2-only conditions compared to hormone-free control, and 250 genes that show significant differential expression in P4-treated conditions compared to E2-only conditions. From this list, we found hormone-induced changes in transcriptional profiles for genes across several classes of mucus production, including ion channels and enzymes involved in post-translational mucin modification that have not previously been described as hormonally regulated. Conclusion: Our study is the first to use an in vitro culture system to create an epithelial-cell specific transcriptome of the endocervix. As a result, our study identifies new genes and pathways that are altered by sex-steroids in cervical mucus production.

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Section: Resultsmentioning

confidence: 99%

Transcriptional profiling of mucus production and modification in rhesus macaque endocervical cells under hormonal regulation

Rapp,

Wei,

Roberts

et al. 2023

Preprint

Self Cite

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“…Additionally, progestins alter the crystal formation (ferning) and stretchability (spinnbarkeit) status of cervical mucus produced by ectocervical cells that may have negative impact on sperm penetration [26, 27]. A recent study showed that P 4 also decreases expression of ion channel genes that modulate cervical mucus properties [28]. However, whether or not changes in mucus properties after treatment with progestin have a direct impact on sperm penetration and migration still needs to be evaluated in controlled and systemic studies with a large sample size [29].…”

Section: Female Hormonal Methodsmentioning

confidence: 99%

Reversible female contraceptives: historical, current, and future perspectives

Barton,

Erickson,

Allred

et al. 2023

Biology of Reproduction

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Contraception is a practice with extensive and complicated social and scientific histories. From cycle tracking, to the very first prescription contraceptive pill, to now having over-the-counter contraceptives on demand, family planning is an aspect of healthcare that has undergone and will continue to undergo several transformations through time. This review provides a comprehensive overview of current reversible hormonal and non-hormonal birth control methods as well as their mechanism of action, safety, and effectiveness specifically for individuals who can become pregnant. Additionally, we discuss the latest Food and Drug Administration (FDA)-approved hormonal method containing estetrol and drospirenone that has not yet been used worldwide as well as the first FDA-approved hormonal over-the-counter progestin-only pills. We also review available data on novel hormonal delivery through microchip, microneedle, and the latest FDA-approved non-hormonal methods such as vaginal pH regulators. Finally, this review will assist in advancing female contraceptive method development by underlining constructive directions for future pursuits. Information was gathered from the NCBI and Google Scholars databases using English and included publications from 1900 to present. Search terms included contraceptive names as well as efficacy, safety, and mechanism of action. In summary, we suggest that investigators consider the side effects and acceptability together with the efficacy of contraceptive candidate towards their development.

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Hormonal regulation of non-cystic fibrosis transmembrane conductance regulator ion channels in the endocervix

Cited by 2 publications

References 42 publications

Transcriptional profiling of mucus production and modification in rhesus macaque endocervical cells under hormonal regulation

Transcriptional profiling of mucus production and modification in rhesus macaque endocervical cells under hormonal regulation

Reversible female contraceptives: historical, current, and future perspectives

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