Hormonal regulation of the androgen receptor expression in human prostatic cells in culture

Blanchere, Marie; Berthaut, Isabelle; Portois, M. C.; Mestayer, Chidi; Mowszowicz, Irène

doi:10.1016/s0960-0760(98)00056-9

Cited by 44 publications

(35 citation statements)

References 36 publications

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“…This close relationship between the two classes of receptor has been demonstrated in cultured human prostatic stromal cells in which exposure to estradiol also raised the level of AR. 6 A similar phenomenon has also been reported in cultured prostatic carcinoma cell lines, 28 in stromal cells of prostatic carcinoma from patients treated with androgen ablation therapy 10 and in castrated dogs treated with oestrogen. 5 Culture of stromal cells with different concentrations of estradiol and testosterone, produced no consistent pattern of ERa and AR expression between the various treatments.…”

Section: Discussionsupporting

confidence: 64%

Upregulation of estrogen and androgen receptors modulate expression of FGF-2 and FGF-7 in human, cultured, prostatic stromal cells exposed to high concentrations of estradiol

Smith

Rhodes

et al. 2002

Prostate Cancer Prostatic Dis

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This study was performed to develop an experimental model in which expression of estrogen receptors (ER) by human prostatic stromal cells could be reproducibly enhanced relative to similar cells with low ER expression. The second aim was to characterise changes in expression of ER, androgen receptor (AR), FGF-2 and FGF-7 in stromal cells exposed to high and low concentrations of estradiol and testosterone mimicking the different sex hormone levels between young and elderly men. Five strains of human prostatic stromal cells, isolated from BPH resections, were grown in steroid-free medium plus 1 mmol 17b-estradiol. After 10 days, cells were passaged and grown in the same medium without estradiol until confluent. In a second study four cell strains were exposed to high and low concentrations of 17b-estradiol and testosterone for 10 days. Cells were labelled with fluorescent antibodies to ERa, AR, FGF-2 and FGF-7 and the fluorescence intensity measured by flow cytometry. Following exposure to 1 mmol estradiol, stromal cells showed reduced expression of AR and ERa but after passage without estradiol they showed a 25% increase in both receptors over controls. Different combinations of sex hormones induced inconsistent changes with respect to expression of ER, AR and FGFs in the various cell-strains. However, there was a highly significant correlation between AR, ER and FGF-2 and FGF-7, which was cell strain-specific. Thus, changes in sex hormone balance per se may not be solely responsible for the observed increases in prostatic ER levels in BPH. Since expression of ER is correlated with synthesis of FGF-2 and FGF-7, it is likely that increases in stromal ER may mediate the synthesis of stromallyderived growth factors which contribute to the aetiopathogenesis of benign prostatic hyperplasia.

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Section: Discussionsupporting

confidence: 64%

Upregulation of estrogen and androgen receptors modulate expression of FGF-2 and FGF-7 in human, cultured, prostatic stromal cells exposed to high concentrations of estradiol

Smith

Rhodes

et al. 2002

Prostate Cancer Prostatic Dis

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“…There were a few reports about the regulation of androgen on AR expression, which is different in individual cells, such as smooth-muscle cells, 22 prostate cancer cells, 23,24 osteoblast-like cells, 25 bone cells 26 and several rat organs. 27 In our TM4/AR cells, AR protein level decreased when treated with T (data not shown), although the mRNA levels of AR were constant.…”

Section: Discussionmentioning

confidence: 99%

Identification of testosterone-/androgen receptor-regulated genes in mouse Sertoli cells

Zhang¹,

Zhang²,

Zhang³

et al. 2011

Asian J Androl

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“…The growth-promoting effect of androgens on the epithelium during development is mediated through an AR-positive stroma. 32 Blanchere et al 33 reported that both androgen and oestrogen increased AR expression in cultured prostatic stromal cells. Our data show that androgen treatment promoted AR expression to a greater degree in PZsc than in TZsc, but that oestrogen had the reverse effect, stimulating more AR expression in TZsc than in PZsc.…”

Section: Discussionmentioning

confidence: 99%

The differential effects of prostate stromal cells derived from different zones on prostate cancer epithelial cells under the action of sex hormones

Jiang

Han²,

Zhao³

et al. 2011

Asian J Androl

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It is well known that prostate cancer (PCa) occurs predominantly in the peripheral zone (PZ), whereas benign prostatic hyperplasia (BPH) typically develops in the transition zone. To identify possible mechanisms underlying zonal differences, we compared the effects of prostate stromal cells derived from the peripheral zone (PZsc) and the transition zone (TZsc) on a PCa epithelial cell line (PC3) in the presence of sex hormones. First, we observed that androgen receptor (AR) mRNA was more highly expressed in PZsc than TZsc when the cells were treated with dihydrotestosterone (DHT) and b-oestradiol (E2) (P,0.05). By ELISA, we looked for differences in the secretion of peptide growth factors from PZsc and TZsc. We found that keratinocyte growth factor (KGF) secretion increased with increasing concentrations of DHT (P,0.01) and was higher in PZsc than TZsc. Under treatment with DHT plus E2, PZsc secreted more transforming growth factor-b1 (TGF-b1) than TZsc, but this pattern was reversed when the cells were treated with E2 only. With increasing concentrations of DHT, insulin-like growth factor-1 (IGF-1) secretion increased in PZsc but decreased in TZsc. To further characterize the effects of PZsc and TZsc on PC3 cells, we developed a coculture model and performed MTT assays, Western blot analysis and real-time RT-PCR. We found that PZsc promoted PC3 cell proliferation and progression better than TZsc, particularly when treated with 10 nmol l 21 DHT plus 10 nmol l 21 E2. In conclusion, our data suggest that PZsc may have a greater capacity to induce PCa development and progression than TZsc via growth factors regulated by sex hormones. These findings provide possible mechanisms underlying zonal differences in prostate diseases, which may aid the search for novel therapeutic targets for PCa.

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Hormonal regulation of the androgen receptor expression in human prostatic cells in culture

Cited by 44 publications

References 36 publications

Upregulation of estrogen and androgen receptors modulate expression of FGF-2 and FGF-7 in human, cultured, prostatic stromal cells exposed to high concentrations of estradiol

Upregulation of estrogen and androgen receptors modulate expression of FGF-2 and FGF-7 in human, cultured, prostatic stromal cells exposed to high concentrations of estradiol

Identification of testosterone-/androgen receptor-regulated genes in mouse Sertoli cells

The differential effects of prostate stromal cells derived from different zones on prostate cancer epithelial cells under the action of sex hormones

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