Hormonal regulation of uterine natural killer cells in mouse preimplantation uterus

Kuang, Haibin; Peng, Hongying; Xu, Hong; Zhang, Baoping; Peng, Jing; Tan, Yi

doi:10.1007/s10735-010-9256-8

Cited by 13 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…uNK cells detection was performed as described previously [18]. Firstly, sections were deparaffinized in xylene, and rehydrated then treated with 3% hydrogen peroxide in PBS for 10 min to block endogenous peroxidase activity.…”

Section: Methodsmentioning

confidence: 99%

Flutamide ameliorates uterine decidualization and angiogenesis in the mouse hyperandrogenemia model during mid-pregnancy

Gong

et al. 2019

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Background/Aim Patients with polycystic ovary syndrome (PCOS), characterized by anovulation, hyperandrogenemia and polycystic ovaries, are still vulnerable to undergo recurrent pregnancy loss and premature labor even though the ovulatory process is pharmacologically recovered. However, its potential mechanism remains unknown. Thus, our aim was to investigate the effect and mechanism of hyperandrogenemia and flutamide (a non-steroidal anti-androgen) on the embryo implantation and pregnancy during mid-pregnancy. Methods We used a mouse model in which PCOS-like hyperandrogenemia was induced by subcutaneous injection of testosterone propionate. In this model, we observed the effect of hyperandrogenemia and flutamide on the decidualization, angiogenesis and uNK cells by methods of immunohistochemistry, quantitative PCR, western blotting and Dolichos biflorus agglutinin (DBA) lectin staining. Results Testosterone and flutamide treatment did not significantly influence the numbers of implanted embryo compared with the control group. However, different doses of testosterone significantly increased the ratio of resorbed /implanted embryo, decreased the level of prl8a2 mRNA and cyclin D3 protein, inhibited the uterine angiogenesis and reduced the numbers of uNK cells, but combined treatment with flutamide markedly decreased the resorbed embryos, increased expressions of prl8a2 mRNA and cyclin D3 protein and angiogenesis and numbers of uNK cells. Conclusion Flutamide treatment can efficiently ameliorate the hyperandrogenemia-induced the disorders in aspects of decidualization, angiogenesis and uNK cells, which further improve the poor endometrial receptivity in PCOS patients.

show abstract

Section: Methodsmentioning

confidence: 99%

Flutamide ameliorates uterine decidualization and angiogenesis in the mouse hyperandrogenemia model during mid-pregnancy

Gong

et al. 2019

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…Their origin or expansion remains a matter of discussion. They may migrate from the periphery, differentiating from NK cell progenitors under the control of different factors, including steroid hormones (55), and/or by recruitment of peripheral NK cells into the uterus (56–58) or expand in situ after pregnancy was established (59). Qu and colleagues demonstrated a P4-dependent osteopontin expression in human decidual stroma cells and human uNK cells and proposed a role for osteopontin in uNK cell accumulation in uterine tissue (60).…”

Section: Hormonal Influence On Immune Cells During Pregnancymentioning

confidence: 99%

Endocrine Factors Modulating Immune Responses in Pregnancy

2014

View full text Add to dashboard Cite

How the semi-allogeneic fetus is tolerated by the maternal immune system remains a fascinating phenomenon. Despite extensive research activity in this field, the mechanisms underlying fetal tolerance are still not well understood. However, there are growing evidences that immune–immune interactions as well as immune–endocrine interactions build up a complex network of immune regulation that ensures fetal survival within the maternal uterus. In the present review, we aim to summarize emerging research data from our and other laboratories on immune modulating properties of pregnancy hormones with a special focus on progesterone, estradiol, and human chorionic gonadotropin. These pregnancy hormones are critically involved in the successful establishment, maintenance, and termination of pregnancy. They suppress detrimental maternal alloresponses while promoting tolerance pathways. This includes the reduction of the antigen-presenting capacity of dendritic cells (DCs), monocytes, and macrophages as well as the blockage of natural killer cells, T and B cells. Pregnancy hormones also support the proliferation of pregnancy supporting uterine killer cells, retain tolerogenic DCs, and efficiently induce regulatory T (Treg) cells. Furthermore, they are involved in the recruitment of mast cells and Treg cells into the fetal–maternal interface contributing to a local accumulation of pregnancy-protective cells. These findings highlight the importance of endocrine factors for the tolerance induction during pregnancy and encourage further research in the field.

show abstract

“…Uterine NK cells did not appear in mice uterus on day 2 of pregnancy, a time with low or no estrogen and progesterone. However, they began to distribute in uterine blood vessels in next day 3 and 4 of pregnancy and the distributing pattern of them is identical to the ovariectomized mice after administration of estrogen and progesterone, which further confirmed their role in homing of uNK cells during early pregnancy [ 17 ].…”

Section: Introductionmentioning

confidence: 74%

The regulation of ovary and conceptus on the uterine natural killer cells during early pregnancy

Gong

Chen

et al. 2017

Reprod Biol Endocrinol

Self Cite

View full text Add to dashboard Cite

Uterine natural killer (uNK) cells are short-lived, terminally differentiated and the most abundant lymphocytes in the uterus which play a crucial role in the spiral arteriole modification and establishment of successful pregnancy. Dysregulation of uNK cells has been linked to gestational implications such as recurrent pregnancy loss, preeclampsia and fetal growth retardation. There is evidence showing that progesterone and estrogen can regulate the recruitment, proliferation, differentiation and function of uNK cells via direct action on intracellular nuclear receptors or through intermediary cells in the uterus during early pregnancy. As the deepening of related research in this field, the role of conceptus in such regulation has received extensive attention, it utilizes endocrine signaling (hCG), juxtacrine signaling (HLA-C, HLA-E, HLA-G) and paracrine signaling (cytokines) to facilitate the activities of uNK cells. In addition, under the influence of ovarian hormones, conceptus can increase expression of PIBF and HLA-G molecules to reduce cytotoxicity of uNK cells and promote angiogenesis. In this review, we aim to concentrate on the novel findings of ovarian hormones in the regulation of uNK cells, emphasize the regulatory role of conceptus on uNK cells and highlight the proposed issues for future research in the field.

show abstract

Hormonal regulation of uterine natural killer cells in mouse preimplantation uterus

Cited by 13 publications

References 17 publications

Flutamide ameliorates uterine decidualization and angiogenesis in the mouse hyperandrogenemia model during mid-pregnancy

Flutamide ameliorates uterine decidualization and angiogenesis in the mouse hyperandrogenemia model during mid-pregnancy

Endocrine Factors Modulating Immune Responses in Pregnancy

The regulation of ovary and conceptus on the uterine natural killer cells during early pregnancy

Contact Info

Product

Resources

About