Hormone Receptor Levels and Hormone, Rna and Protein Metabolism in the Genital Tract During the Oestrous Cycle of the Ewe

Miller, BG; Murphy, Leigh C.; Stone, G. M.

doi:10.1677/joe.0.0730091

Cited by 54 publications

(36 citation statements)

References 19 publications

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“…The initial effect of estradiol on receptor levels was inhibitory, but the subsequent response was to increase receptor expression. The initial suppression on the binding capacity of ER and PR is different from the reported receptor up-regulation induced by estrogen in cyclic and ovariectomized ewes [25,31,32]. It has been suggested that E2 regulation of uterine ER in rat is dose dependent, as demonstrated by the positive and negative effects of low and high doses of E2 on ER concentrations, respectively [20].…”

Section: Discussionmentioning

confidence: 89%

“…An example of this action is the regulation of the expression of their own receptor; the E-receptor complex stimulates both ER and PR gene expression in uteri of several mammalian species, while the P-receptor complex reduces levels of both receptors [4]. This is displayed by the variations in the concentrations of uterine sex steroid receptors [25,31] as well as their respective mRNAs during the estrous cycle of the sheep [27,35].…”

Section: Introductionmentioning

confidence: 99%

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A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

et al. 2000

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-Regulation of the uterine expression of estrogen and progesterone receptors was studied in 20 three-month-old lambs that were not treated or treated with estradiol-17β. Determinations of receptors were performed by binding assays in the nuclear and cytosolic fractions, receptor mRNAs by solution hybridization, and estrogen receptor protein by an enzyme-immunoassay. Estradiol treatment decreased the receptor binding capacity of both receptors and the levels of immunoreactive estrogen receptor 12 h after injection in the absence of decreased receptor mRNAs, suggesting that the initial decrease is due to degradation of the proteins or that mRNAs are translated into new receptor proteins at a reduced rate. The mRNA levels increased after estradiol treatment suggesting that the replenishment phase consists of synthesis of new receptors rather than recycling of inactivated receptors.estrogen / progesterone / receptor / uterus / lamb Résumé -Action biphasique de l'oestradiol sur l'expression des récepteurs aux oestrogènes et à la progestérone dans l'utérus de l'agnelle. La régulation de l'expression des récepteurs aux oestrogènes et à la progestérone au niveau de l'utérus a été étudiée chez des agnelles immatures âgées de 3 mois (n = 20), traitées et non traitées avec oestradiol-17β. Les déterminations des récepteurs ont été faites par des essais de liaison dans les fractions nucléaires et cytosoliques, les ARNm des récepteurs par la méthode d'hybridation en solution et la protéine du récepteur aux oestrogènes par un essai immuno-enzymatique. Le traitement à l'oestradiol réduit la capacité de liaison des deux récepteurs et les niveaux des récepteurs aux oestrogènes immunoréactifs 12 h après l'injection en absence d'une diminution des ARNm des récepteurs, suggèrent que la réduction initiale est due à la dégradation des Reprod. Nutr. Dev. 40 (2000) 283-293 283

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

et al. 2000

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“…A varying portion of epithelium is denuded from the intercotyledonary endometrium by the trophoblast (Amoroso 1952). At days 0 and 28 slices of caruncular endometrium were collected using a Stadie-Riggs microtome (Miller et al 1977). The wall of the chorionic sac could be easily peeled away from caruncles at day 28.…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study we examined some changes that occur in the uterus during the oestrous cycle of the ewe (Miller et al 1977). The in vitro rate of protein synthesis and RNA: DNA ratio in caruncular endometrium were maximal at or shortly after oestrus (days 0-2) and declined to low levels during the late luteal phase of the cycle (days [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Uterine and Placental Growth: RNA and Protein Metabolism and Steroid Hormone Receptor Levels in the Endometrium, Whole Uterus and Cotyledons during Pregnancy in the Ewe

Miller¹,

Stone²

1981

Aust. Jnl. Of Bio. Sci.

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Some aspects of uterine and placental growth have been examined during pregnancy in the ewe. Changes in in vitro rates of protein synthesis, RNA:DNA and protein:DNA ratios and the tissue concentration of DNA in intercaruncular endometrium and caruncles/cotyledons between days 0 (oestrus) and 112 of pregnancy were compared with corresponding changes in the concentrations of high-affinity cytosol receptors for oestradiol and progesterone in whole uterus and caruncles/maternal cotyledons. Rapid growth of the intercaruncular endometrium between days 28 and 112 and of the developing cotyledons between days 28 and 84 occur in the presence of tissue levels of both steroid receptors that are extremely low in relation to the corresponding levels seen in the uterus at oestrus. If uterine responses to steroid hormones are regulated by the amounts of specific receptor present in the tissue, the results support the concept that uterine growth after day 28 of pregnancy results primarily from the physical stimulus of the growing conceptus rather than from the actions of endogenous steroid sex hormones.

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“…The level of 'total' cytosol receptors for E2 and P were measured generally as previously described (Miller et al 1977b; except that for mice, rabbit and guinea pig tissue the phosphate in all buffers was replaced by 0·01 M Tris-HCl, pH 7 ·4. Five grams of minced sheep uterus were homogenized in 3 vol.…”

Section: Assay Of Cytosol Receptors For Oestradiol and Progesteronementioning

confidence: 99%

Effects of Progesterone on the Oestrogen-Stimulated Uterus: a Comparative Study of the Mouse, Guinea Pig, Rabbit and Sheep

Miller¹,

Wild²,

Stone³

1979

Aust. Jnl. Of Bio. Sci.

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To examine more closely the anti-oestrogenic action of progesterone (P), its effect on various parameters in the 17 fl-oestradiol (E2)-primed uterus of the mouse, guinea pig, rabbit and ewe was studied. Changes in uterine wet weight, rate of in vitro protein synthesis, protein: DNA and RNA :. DNA ratios, peroxidase activity and the level of cytosol receptors for E2 and P were measured. Considerable between-species differences in the effect of P on these parameters were observed. The anti-uterotrophic action was greater in the mouse than in the guinea pig and was not seen in the rabbit or ewe. P inhibited protein synthesis in the mouse, was without significant effect in the guinea pig and was mildly stimulatory in the rabbit and ewe. Inhibitory effects on protein: DNA and RNA: DNA ratios were substantial in the mouse, minor in the guinea pig and absent in the rabbit and ewe. Peroxidase activity was decreased in the mouse and guinea pig, essentially lacking in the rabbit and not detectable in the ewe. In all species the level of both oestrogen and progesterone cytosol receptors was decreased, although the effect on the E2 receptor was less marked in the ewe.The results indicate that in the species studied an effect of P on the replenishment of the E2 receptor is not necessarily associated with other anti-oestrogenic actions of P and argue against the concept that initial anti-oestrogenic actions of P are mediated via a specific effect on such replenishment. IntroductionIn the ovariectomized rat or mouse progesterone (P) substantially inhibits the rapid increases in RNA and protein accumulation and uterine weight that are seen in animals receiving large doses of oestrogen only (Roberts and Szego 1953;Martin 1963;Miller and Emmens 1969;Miller 1978). P also diminishes the cytosol concentration of specific, high-affinity 17 p-oestradiol (E 2 ) receptor in the uterus of these species, and several authors have suggested that the anti-oestrogenic or anti-uterotrophic activity of P may be mediated by this particular action of P on the E2 receptor (Bhakoo and Katzenellenbogen 1977;Clark et al. 1977;Coulson and Pavlik 1977).When ovariectomized ewes receive P together with large doses of E2 the P does not interfere with oestrogen-induced increases in uterine weight and endometrial RNA: DNA ratios (Miller 1976;Miller et al. 1977a). In these studies P caused only a small decrease in the cytosol concentration of E2 receptor in whole uterus. Because of these findings, we suggested that the apparent inability of P to much reduce the uterine level of E2 receptor in the ewe may explain the absence of other anti-oestrogenic actions of P in this species.

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Hormone Receptor Levels and Hormone, Rna and Protein Metabolism in the Genital Tract During the Oestrous Cycle of the Ewe

Cited by 54 publications

References 19 publications

A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

Uterine and Placental Growth: RNA and Protein Metabolism and Steroid Hormone Receptor Levels in the Endometrium, Whole Uterus and Cotyledons during Pregnancy in the Ewe

Effects of Progesterone on the Oestrogen-Stimulated Uterus: a Comparative Study of the Mouse, Guinea Pig, Rabbit and Sheep

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