Hormone Receptor Levels and Metabolic Activity in the Uterus of the Ewe: Regulation by Oestradiol and Progesterone

Stone, G. M.; Murphy, Leigh C.; Miller, BG

doi:10.1071/bi9780395

Cited by 19 publications

(10 citation statements)

References 16 publications

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“…An explanation for the difference between these results may lie in the techniques applied (isoelectric focussing, Roberts et al, 1976; SDS-PAGE size separation, present study), or in the failure of the technique used to resolve labelled proteins destined for export; the majority of proteins labelled were confined to tissue and were not recovered in the supernatant. Uterine tissues from pregnant or non-pregnant ewes, or from ovariectomized ewes treated with ovarian steroids, showed negligible metabolism of androstenedione to phenolic compounds, a finding noted previously (Rossier & Pierrepoint, 1974;Murphy, Stone, Miller & Moore, 1977;Stone, Murphy & Miller, 1978). Both oestradiol-17ß (Greiss & Miller, 1971;Anderson, Hackshaw, Still & Greiss, 1977) and the presence of a conceptus (Greiss & Anderson, 1970) increased uterine blood flow at Days 13-15 of pregnancy in sheep.…”

Section: Discussionsupporting

confidence: 57%

Protein, prostaglandin and steroid synthesis in caruncular and intercaruncular endometrium of sheep before implantation

Findlay¹,

Ackland²,

Burton³

et al. 1981

Reproduction

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The effect of the presence of a preimplantation embryo on protein concentration, rate of protein synthesis, beta-glucuronidase and acid phosphatase activities, steroid metabolism and prostaglandin F production in caruncular and intercaruncular tissue have been studied for sheep at Day 15 of pregnancy. The rate of protein synthesis in both tissues was greater in pregnant than in non-pregnant animals, although the difference was only significant in caruncular endometrium. The effect in caruncular tissue was mimicked in ovariectomized animals treated with oestradiol. Localized changes in the caruncular tissue were observed in respect of PGF with an increased tissue concentration, an enhanced basal release when the tissue was incubated in the presence of indomethacin, and a decreased net production. Maximum production of PGF in the 2 tissues was unaffected by the presence of an embryo but it was enhanced by oestradiol or progesterone treatment in intercaruncular tissue of ovariectomized ewes. beta-Glucuronidase and acid phosphatase activities and steroid metabolism were unaffected by pregnancy. However, in ovariectomized animals oestradiol treatment stimulated beta-glucuronidase activity in endometrium and myometrium. Progesterone treatment stimulated acid phosphatase activity in the intercaruncular endometrium. The results show that amongst several endometrial constituents investigated relatively few changes were detected by Day 15 post coitum, one day before definitive attachment. Those changes that did occur were associated with the dynamics of PGF production and the rate of protein synthesis, and were consistent with the production of a PGF binding component in caruncular endometrium which may be concerned with the protection of luteal function by redirection of uterine PGF production. Canonical variate analysis revealed that changes on Day 15 of pregnancy were mimicked most closely in caruncular tissue by treatment of ovariectomized ewes with oestradiol and progesterone, and in intercaruncular tissue by oestradiol treatment only.

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Section: Discussionsupporting

confidence: 57%

Protein, prostaglandin and steroid synthesis in caruncular and intercaruncular endometrium of sheep before implantation

Findlay¹,

Ackland²,

Burton³

et al. 1981

Reproduction

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“…In both experiments, the number of unfilled sites in ovariectomized ewes was increased within 24 hr after an injection of estradiol. Estradiol-induced replenishment of estradiol binding sites has been reported for ovine endometrium (Koligian and Stormshak, 1977a) and for whole uterus (Stone et al, 1978). Estradiol injections also increased unfilled binding sites in nuclei at 24 hr in Exp.…”

Section: Discussionmentioning

confidence: 66%

“…1. This con-firms, for myometrium, that estradiol increases progestogen binding in cytoplasm and whole uterus (Stone et al, 1978). Progesterone not only decreased estrogen binding receptor replenishment but also reduced concentrations of progestogen binding sites in cytoplasm and nuclei.…”

Section: Discussionmentioning

confidence: 79%

Estrogen and Progestogen Binding in the Myometrium of the Ewe. II. Regulation by Estradiol and Progesterone

Rexroad¹

1981

Journal of Animal Science

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Two experiments were conducted to study the role of estradiol and progesterone in regulating steroid binding sites in sheep uterine myometrium. Exogenous estradiol increased the concentration of estradiol binding sites in nuclei and subsequently increased the total cell content of estradiol binding sites. Estradiol increased the number of progestogen binding sites (promegestone binding) and may have enhanced the translocation of progestogen binding sites to nuclei. Progesterone decreased the number of progestogen binding sites and blocked estradiol-induced increase in cytoplasmic estradiol binding sites. Progesterone did not block the estradiol-induced increase in estradiol binding sites in nuclei. Estradiol increases myometrial RNA to DNA ratios with in 24 hr of a single injection, whereas progesterone did not. Progesterone blocked the estradiol-induced increase in RNA:DNA. Estradiol apparently promotes the synthesis of both types of binding site. Progesterone, on the other hand, appears to inhibit estrogen-induced synthesis of steroid binding sites by blocking estradiol action at the nucleus. Progesterone may also decrease the number of progestogen binding site through a mechanism other than its "anti-estrogenic" action.

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“…In the ewe, the concentration of oestrogen receptors is influenced largely by circulating levels of oestrogen and pro¬ gesterone (Stone, Murphy & Miller, 1978). As the ewes in our previous study were ovariectomized, the differences in the concentrations of receptors may have been the result of differences in the concen¬ trations of extragonadal steroids, presumably of adrenal origin.…”

Section: Introductionmentioning

confidence: 92%

Adrenal glands alter the concentration of oestradiol-17β and its receptor in the uterus of ovariectomized ewes

Atkinson

Adams²

1988

Journal of Endocrinology

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To investigate the effects of adrenal hormones on oestrogen activity in the uterus, ovariectomized ewes were either adrenalectomized, administered glucocorticoid-like preparations (CORT), or remained as controls. The adrenalectomized ewes were maintained with a corticoid-replacement therapy and monitored daily for plasma glucose and Na+/K+ concentrations. Blood samples were collected from all ewes at 15-min intervals for 4 h and assayed for LH and FSH. The adrenalectomized ewes were killed 9 days after adrenalectomy, while the CORT ewes were killed after 3 weeks of drug therapy. The control ewes were killed simultaneously with the CORT ewes. Uterine tissues were homogenized and the numbers of oestradiol receptors and tissue concentrations of oestradiol-17 beta were measured. The adrenalectomized ewes had significantly higher concentrations of cytosolic oestrogen receptors in the uterus than did the control ewes, which had significantly higher concentrations than the CORT ewes (0.95 +/- 0.06, 0.76 +/- 0.02 and 0.60 +/- 0.02 (S.E.M.) mumol/kg protein respectively). The concentrations of oestradiol-17 beta measured in uterine tissues were significantly lower in adrenalectomized and CORT ewes than in control ewes (37.4 +/- 5.5, 61.7 +/- 25.7 and 135.5 +/- 12.8 pmol/kg respectively). There were no significant differences between any groups in the peripheral concentrations of LH or FSH. These results indicate that the adrenal gland affects the concentrations of both oestrogen and its receptor in the uterus of ovariectomized ewes, probably by different mechanisms. These effects are not mediated by gonadotrophins.

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Hormone Receptor Levels and Metabolic Activity in the Uterus of the Ewe: Regulation by Oestradiol and Progesterone

Cited by 19 publications

References 16 publications

Protein, prostaglandin and steroid synthesis in caruncular and intercaruncular endometrium of sheep before implantation

Protein, prostaglandin and steroid synthesis in caruncular and intercaruncular endometrium of sheep before implantation

Estrogen and Progestogen Binding in the Myometrium of the Ewe. II. Regulation by Estradiol and Progesterone

Adrenal glands alter the concentration of oestradiol-17β and its receptor in the uterus of ovariectomized ewes

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