Hormone receptor status in breast cancer – a comparison between surgical specimens and fine needle aspiration biopsies

Löfgren, Lars; Skoog, Lambert; Schoultz, E. von; Tani, Edneia; Isaksson, Eva; Fernstad, Roland; Carlström, Kjell; Schoultz, Bo von

doi:10.1046/j.1365-2303.2003.00054.x

Cited by 26 publications

(15 citation statements)

References 23 publications

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“…Many studies have compared the results of IHC staining for breast carcinoma biomarkers performed on cytologic smears and liquid-based preparations to those performed on tissue samples [6,7,8,9,10,11,12]. Numerous studies have investigated the effects of different fixation methods on both cytologic and histologic samples of breast carcinoma [13,14,15,16,17,18,19,20].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Breast Carcinoma Prognostic/Predictive Biomarkers on Cell Blocks Obtained by Various Methods: Cellient, Formalin and Thrombin

et al. 2012

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Objective: To compare results of immunohistochemical (IHC) assays for estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) performed on thrombin, formalin and Cellient cell blocks to those performed on tissue. Study Design: Formalin, thrombin and Cellient cell blocks were prepared from cytologic samples obtained from resection specimens of 31 patients with invasive breast carcinoma. ER, PR, HER2 and MIB-1 (Ki-67) IHC stains were performed on all three types of cell blocks and compared to the same stains performed on the patient’s paraffin-embedded biopsy or resection. Cell and tissue blocks with equivocal staining for HER2 were submitted for fluorescence in situ hybridization (FISH). Results: Adequate Cellient blocks were obtained for all 31 cases. Comparison of results of ER IHC assays on all three types of cell blocks showed 100% correlation with tissue. Both Cellient and thrombin blocks showed 100% correlation with tissue for HER2 IHC and FISH results. The only statistically significant difference between cell block methods was found in PR staining, where false-negative results occurred with Cellient and thrombin blocks. Conclusion: Breast biomarker IHC assays performed on Cellient blocks are reliable and correlate with tissue block results, particularly for ER and HER2, the most clinically important markers.

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Section: Discussionmentioning

confidence: 99%

Comparison of Breast Carcinoma Prognostic/Predictive Biomarkers on Cell Blocks Obtained by Various Methods: Cellient, Formalin and Thrombin

et al. 2012

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“…Furthermore, the use of cell blocks has provided material for ancillary studies including the analysis of hormone receptors (ER, PR) and HER2 status, and for immunostains (i.e. cytokeratin) that can help support the presence of metastatic carcinoma in difficult cases [5,6,7,8,9]. In addition, the effectiveness of FNA has been optimized with the use of real-time ultrasound guidance that has helped to target specific areas of concern, to confirm the needle location in the LN, and to facilitate interactions between radiologists and pathologists to improve the quality of the FNA sample [10,11].…”

Section: Introductionmentioning

confidence: 99%

The Role of Axillary Lymph Node Fine Needle Aspiration in Breast Cancer Staging prior to Chemotherapy with Histological Correlation

Colaizzi¹,

Kanbour²,

Ibrahim³

et al. 2012

Acta Cytologica

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Objective: Axillary lymph node (LN) sampling is important for breast cancer staging and can be performed using fine needle aspiration (FNA). The aim of this study is to review the axillary LN FNAs performed at our institution prior to treatment, and to correlate their results with the available LN histology in order to evaluate the diagnostic utility of FNA. Study Design: A total of 130 cases were retrospectively identified over a 1-year period and reviewed. The FNA findings were correlated with the histological findings in 65 (50%) LN core biopsies, 43 (33%) sentinel LN biopsies, and 22 (17%) axillary dissections. Results: One hundred and thirteen FNA cases (89%) correlated with the histology, while 14 cases (11%) did not correlate, including 1 false positive and 13 false negatives. Of the false-negative cases, metastases in the biopsy ranged from isolated tumor cells to 5 mm in the greatest dimension. The overall sensitivity was 85%, specificity 98%, positive predictive value 99%, and negative predictive value 75%. Conclusion: Pretherapy staging of breast cancer by FNA is a useful diagnostic modality with a high specificity and positive predictive value. Due to false-negative diagnoses, a negative FNA should be followed up with further LN sampling.

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“…Staining intensity was not included in the analysis. The reproducibility and diagnostic relevance of this parameter has been questioned [13,14]. The results from image analysis were compared with manual scoring of cells with a positive or negative staining (two different observers blinded to treatment).…”

Section: Image Analysismentioning

confidence: 99%

Expression of sex steroid receptor subtypes in normal and malignant breast tissue – a pilot study in postmenopausal women

et al. 2006

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Hormone receptor status in breast cancer – a comparison between surgical specimens and fine needle aspiration biopsies

Cited by 26 publications

References 23 publications

Comparison of Breast Carcinoma Prognostic/Predictive Biomarkers on Cell Blocks Obtained by Various Methods: Cellient, Formalin and Thrombin

Comparison of Breast Carcinoma Prognostic/Predictive Biomarkers on Cell Blocks Obtained by Various Methods: Cellient, Formalin and Thrombin

The Role of Axillary Lymph Node Fine Needle Aspiration in Breast Cancer Staging prior to Chemotherapy with Histological Correlation

Expression of sex steroid receptor subtypes in normal and malignant breast tissue – a pilot study in postmenopausal women

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