Hormone replacement therapy leads to increased plasma levels of platelet derived microparticles in postmenopausal women

Rank, Andreas; Nieuwland, Rienk; Nikolajek, Katharina; Rösner, Sabine; Wallwiener, Lisa-Maria; Hiller, Erhard; Tóth, Bettina

doi:10.1007/s00404-011-2098-0

Cited by 17 publications

(12 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In line with this conclusion, we found the highest counts of CD61-exposing MP in premenopausal women who took estrogen-containing contraceptives, even though the number of investigated women is small and limits the validity of our data. But the idea of a relevant influence of female hormones on the plasma level of CD61+ MP is supported by recent published data from our group: this subtype of plasma MP is higher in postmenopausal women with hormone replacement therapy compared to those without hormone replacement therapy [28]. The general relevance of estrogens becomes evident by the fact that women have higher mean counts of platelets as well as of CD61-exposing MP – probably mainly derived from megakaryocytes – than men [29].…”

Section: Discussionsupporting

confidence: 68%

Climacteric Lowers Plasma Levels of Platelet-Derived Microparticles: A Pilot Study in Pre- versus Postmenopausal Women

Rank¹,

Nieuwland²,

Roesner³

et al. 2012

Acta Haematol

Self Cite

View full text Add to dashboard Cite

Background: Climacteric increases the risk of thrombotic events by alteration of plasmatic coagulation. Up to now, less is known about changes in platelet- (PMP) and endothelial cell-derived microparticles (EMP). Methods: In this prospective study, plasma levels of microparticles (MP) were compared in 21 premenopausal and 19 postmenopausal women. Results: No altered numbers of total MP or EMP were measured within the study groups. However, the plasma values of CD61-exposing MP from platelets/megakaryocytes were higher in premenopausal women (5,364 × 10⁶/l, range 4,384–17,167) as compared to postmenopausal women (3,808 × 10⁶/l, range 2,009–8,850; p = 0.020). This differentiation was also significant for the subgroup of premenopausal women without hormonal contraceptives (5,364 × 10⁶/l, range 4,223–15,916; p = 0.047; n = 15). Furthermore, in premenopausal women, higher plasma levels of PMP exposing CD62P were also present as compared to postmenopausal women (288 × 10⁶/l, range 139–462, vs. 121 × 10⁶/l, range 74–284; p = 0.024). This difference was also true for CD63+ PMP levels (281 × 10⁶/l, range 182–551, vs. 137 × 10⁶/l, range 64–432; p = 0.015). Conclusion: Climacteric lowers the level of PMP but has no impact on the number of EMP in women. These data suggest that PMP and EMP do not play a significant role in enhancing the risk of thrombotic events in healthy, postmenopausal women.

show abstract

Section: Discussionsupporting

confidence: 68%

Climacteric Lowers Plasma Levels of Platelet-Derived Microparticles: A Pilot Study in Pre- versus Postmenopausal Women

Rank¹,

Nieuwland²,

Roesner³

et al. 2012

Acta Haematol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Lowered levels are described in postmenopausal women with decreased estradiol levels, hormone replacement therapy reverses this effect, though (222,223).…”

Section: Distribution Of Pl-evsmentioning

confidence: 99%

Platelet‐derived extracellular vesicles in plateletpheresis concentrates as a quality control approach

et al. 2015

View full text Add to dashboard Cite

BACKGROUND Platelet‐derived extracellular vesicles (PL‐EVs) are present in plateletpheresis concentrates (PCs) and may influence the quality of PCs. The aim of the study was to analyze PC‐derived PL‐EVs and to correlate them with standard quality control (QC) variables of PCs and with donor‐specific laboratory variables. STUDY DESIGN AND METHODS PL‐EVs were analyzed by standard as well as advanced high‐sensitivity flow cytometry (FCM) and nanoparticle tracking analysis. A hematology analyzer was applied to the determination of platelet (PLT) count and immature PLT fraction (IPF). Functional capacity of PLTs (CD62P in response to thrombin receptor‐activating peptide 6 activation) was measured by FCM. All in vitro measurements were carried out on Day 0 and on Day 5. Altogether, a total of 42 PC samples, 15 irradiated on Day 0, were investigated. RESULTS Externalization of CD62P, as an indicator of intact PLT function, significantly decreased during in vitro PLT senescence and CD62P expression inversely correlated with increased PL‐EV levels. Interestingly, in fresh PCs a significant correlation was found between PL‐EVs and different hemapheresis instruments, duration of apheresis, and IPF count in peripheral blood of the donor before apheresis. In senescent PCs, the body mass index of donors inversely correlated with the PL‐EV counts. CONCLUSION Loss of PLT function in PCs was associated with increased PL‐EV levels. Shedding of PL‐EVs depends on shear stress influenced by different hemapheresis settings and diverse preanalytical conditions of donors. PL‐EV analysis may stimulate new quality and apheresis strategies for more vital PLTs for transfusion.

show abstract

“…Data in the literature indicate that exogenous female hormones significantly increase the MP production from platelets and granulocytes in post-menopausal women [38]. Thus, because of the thrombogenicity of hormonal contraception, it was expected that higher MP activity would be found in the OC(+) group than in the OC(-) group, but in the current study, the reverse was found.…”

Section: Discussionmentioning

confidence: 45%

Early response of selected haemostatic and haematological parameters to physical activity in young women – the potential impact of oral contraceptives

Jastrzębska¹,

Żyżniewska-Banaszak²,

Nawrot³

et al. 2017

J Pre Clin Clin Res.

View full text Add to dashboard Cite

Early response of selected haemostatic and haematological parameters to physical activity in young women -the potential impact of oral contraceptives. J Pre Clin-Clin Res. 2017; 11(1): 15-21. doi: 10.26444/jpccr/75148 Abstract Introduction and objective. Exercise (submaximal) in untrained subjects can modify haemostasis toward hypercoagulability, especially among women using oral contraceptives (OC). The aim of this study was to investigate whether this can be explained by platelet haemostasis and changes in the generation of membrane microparticles. Materials and method. Young, healthy women (n=60) were divided into 2 equal groups: a study group OC (+) who had used OC for >3 months, and controls who had never used oral contraceptives OC(-). Exclusion criteria: those with systematic daily physical activity. Participants were subjected to treadmill exercise (Cardiac Diagnostic System; model CH2000) using the Bruce protocol/ AHA guidelines. Platelet aggregation with arachidonic acid (ASPI test) or ADP (ADP test), membrane microparticle (MP) activity, plasma coagulation times (APTT/PT) and blood count were determined before and 45 minutes after exercise. Results. Before exercise, the OC(+) group had slightly higher platelet aggregation (ADP test), significantly lower MP activity, slightly lower PLT and slightly higher PDW rate. Exercise caused slight inhibition of platelet aggregation (ASPI test), and significant decrease in MP activity -only in the OC(-) group. After exercise, in both groups there was a significant decrease in PLT and increase in WBC, more pronounced in OC(+) group. Conclusions. Submaximal exercise was beneficial for haemostasis in women not using hormonal contraception, associated primarily with reduced MP activity. No beneficial effects of physical activity were found for women using hormonal contraceptives, possibly associated with a hypercoagulable state, and higher reactivity of blood platelets under the influence of the use of contraceptives.

show abstract

Hormone replacement therapy leads to increased plasma levels of platelet derived microparticles in postmenopausal women

Cited by 17 publications

References 45 publications

Climacteric Lowers Plasma Levels of Platelet-Derived Microparticles: A Pilot Study in Pre- versus Postmenopausal Women

Climacteric Lowers Plasma Levels of Platelet-Derived Microparticles: A Pilot Study in Pre- versus Postmenopausal Women

Platelet‐derived extracellular vesicles in plateletpheresis concentrates as a quality control approach

Early response of selected haemostatic and haematological parameters to physical activity in young women – the potential impact of oral contraceptives

Contact Info

Product

Resources

About