Hormones and the control of porphyrin biosynthesis and structure in the hamster Harderian gland

Payne, A. P.; Shah, Samyak; Marr, F. A.; McGadey, J; Thompson, George G.

doi:10.1002/(sici)1097-0029(19960601)34:2<123::aid-jemt5>3.0.co;2-t

Cited by 9 publications

(5 citation statements)

References 65 publications

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“…Other authors observed in the HG of rats that most type-I and II glandular cells displayed strong nuclear AR immunoreactivity, and that the intensity of the receptors' reactivity was similar in type-I and II glandular cells [31]. Indeed, the mRNA for the androgen receptor is present in both type-I and II cells, suggesting that type-I and type-II cells are two forms of the same cell [32], probably denoting different activity or secretory statuses [33]. As expected, we have observed, in the HG of the male of M. libycus, an increase in the intensity of the androgen receptors during the breeding period (February to July) compared with the resting period (September to January).…”

Section: Discussionsupporting

confidence: 88%

Structural study and expression of the androgen receptors during the reproductive cycle in the Harderian gland of the male Meriones libycus

Hanniche

Saadi-Brenkia

Lounis

et al. 2019

Comptes Rendus. Biologies

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Section: Discussionsupporting

confidence: 88%

Structural study and expression of the androgen receptors during the reproductive cycle in the Harderian gland of the male Meriones libycus

Hanniche

Saadi-Brenkia

Lounis

et al. 2019

Comptes Rendus. Biologies

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“…Information on vascularization can also be obtained. Further studies are necessary to evaluate whether the methods are sensitive enough for the porphyrin content to be studied (Payne et al. 1996) and for an analysis of sexual dimorphism, age‐related modification and functional activation of the organ.…”

Section: Discussionmentioning

confidence: 99%

“…The mammalian HG is characterized by the production of porphyrins typically found in the lumina as solid accretions. The rodent HG has been extensively used as a model of porphyrin biosynthesis since the presence of porphyrin was described in rat HG long ago (Antolin et al 1996;Baccari, 1996;Chieffi et al 1996;Payne et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Magnetic resonance imaging of the rat Harderian gland

et al. 2002

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The intra-orbital lachrymal gland (Harderian gland, or HG) of the female rat was studied by magnetic resonance imaging (MRI) to evaluate whether MRI can be used to visualize the gland in vivo and localized-1H-spectroscopy detect its lipid content. The results were correlated with post-mortem anatomical sections, and with light and electron microscopy. On MRI, HG presented as a mass located between the ocular bulb and the orbit. In strongly T2W sequences the secretory structures had a reduced signal while intraparenchymal connective tissue was visible.T2-quantitative maps values of HG (60.12 ± 8.15 ms, mean ± SD) were different from other tissues (i.e. muscular tissue, T2 = 44.79 ± 3.43 ms and olfactory bulb, T2 = 79.26 ± 4.25 ms). In contrast-enhanced-MRI, HG had a signal-intensitydrop of 0.074 ± 0.072 (mean ± SD), after injection of AMI-25, significantly different from the muscle (0.17 ± 0.10).Localized MRI spectra gave a large part of the signal originating from fat protons, but with a significant percentage from water protons. At light and electron microscopy the lipid deposition appeared to be composed of low-density material filling a large part of the cytoplasm, and the porphyrin aggregates were easily recognizable. The data demonstrate that an in vivo study of the HG was feasible and that high-field MRI allowed analysis of the gross anatomy detecting the lipid content of the gland.

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“…In situ hybridisation techniques have demonstrated that mRNA for the androgen receptor is present in both type I and type II cells, suggesting that both cell types can respond to circulating androgens (Dominguez et al 1994). These results suggest that type I and type II cells are 2 forms of the same cell, probably denoting different activity or secretory status (Payne et al 1996). Electron microscopically, Lopez et al (1996) did not find any developmental evidence for the existence of separate precursor cells for type I and type II cells in the Syrian hamster harderian gland.…”

Section: mentioning

confidence: 96%

Immunohistochemical characterisation of epithelial cells of rodent harderian glands in primary culture

et al. 1999

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The aims of the current investigation were (1) to establish an efficient procedure for the isolation of rodent harderian gland cells and to define conditions for maintenance of viable differentiated cells ; (2) to compare the in vitro growth pattern of cultured epithelial cells ; and (3) to characterise the cultured epithelial cells from 3 rodent species : Wistar rats, Syrian hamsters and Djungarian hamsters. We have established primary culture conditions that permit the maintenance of viable and differentiated secretory cells from adult rodent harderian gland. This study demonstrates that the cell growth pattern is faster in hamsters than in rats and despite morphological changes, epithelial cells reestablish their distinctive (biochemical\metabolic) phenotype as indicated by lipid-containing vacuoles, porphyrin pigment and serotonin and tryptophan hydroxylase labelling.Key words : Harderian gland ; primary cell culture ; immunohistochemistry ; tryptophan hydroxylase ; serotonin ; vimentin. The harderian gland is an orbital gland located around the posterior part of the eyeball of many terrestrial vertebrates (Sakai, 1981 ;Payne, 1994 ; Chieffi et al. 1996). In rodents, this gland is remarkably large, containing considerable amounts of lipids and porphyrins that are released via a duct which opens onto the nictitating membrane (Djeridane, 1992(Djeridane, , 1994(Djeridane, a,b, 1996. Its potential functions include lubrication of the cornea, production of pheromones and\or thermoregulatory lipids, and a site of immune responses (for reviews see Sakai, 1981 ;Payne, 1994 ; Chieffi et al. 1996). Furthermore, it is also postulated as an extrapineal source of melatonin (Djeridane et al. 1998 (Brownscheidle and Niewenhuis, 1978 ;Payne et al. 1992 ;Schreckenberger and Reuss, 1993 ; Djeridane, 1994 Djeridane, a, 1996. Moreover, we have recently demonstrated by means of immunohistochemistry on tissue sections, using serotonin (melatonin precursor) and tryptophan hydroxylase (the rate-limiting enzyme for serotonin synthesis) antibodies, that the indoleamine synthesis occurs in the type I cell (Djeridane et al. 1999).The purpose of the current study was (1) to establish conditions for the isolation of cells from the harderian gland of Wistar rats, Djungarian hamsters and Syrian hamsters, in order (2) to compare the in vitro growth pattern of the glandular epithelial cells from different rodent species and (3) to determine whether cultured epithelial cells reestablish their phenotype by using morphological\biochemical\metabolic criteria as lipid-containing vacuoles, vacuole size, porphyrins and immunolabelling for serotonin and tryptophan hydroxylase.

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Hormones and the control of porphyrin biosynthesis and structure in the hamster Harderian gland

Cited by 9 publications

References 65 publications

Structural study and expression of the androgen receptors during the reproductive cycle in the Harderian gland of the male Meriones libycus

Structural study and expression of the androgen receptors during the reproductive cycle in the Harderian gland of the male Meriones libycus

Magnetic resonance imaging of the rat Harderian gland

Immunohistochemical characterisation of epithelial cells of rodent harderian glands in primary culture

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