Horseradish and soybean peroxidases: comparable tools for alternative niches?

Abstract

Horseradish and soybean peroxidases (HRP and SBP, respectively) are useful 9 biotechnological tools. HRP is often termed the classical plant heme peroxidase, and 10 although it has been studied for decades our understanding has deepened since its 11 cloning and subsequent expression, which has enabled numerous mutational and 12 protein engineering studies. SBP, however, has been neglected until recently; despite 13 offering a real alternative to HRP that actually outperforms it in terms of stability. 14 SBP is now used in numerous biotechnological applications, including biosensors. 15Review of both is timely. This article summarises and discusses the main insights into 16 the structure and mechanism of HRP, with special emphasis on HRP mutagenesis, and 17 outlines its use in a variety of applications. It also reviews current knowledge and 18 applications to date of SBP, particularly biosensors. The final paragraphs speculate on 19 the future of plant heme-based peroxidases, with probable trends outlined and 20 explored. 21 22

Effects of mutations in the helix G region of horseradish peroxidase

“…The most widely studied peroxidase is isoform C from horseradish roots (Armoracia rusticana; HRP), due mainly to its many uses in biotechnology [1]. Improvement of HRP's catalytic properties and/or stability would further extend its range of applications.…”

“…Improvement of HRP's catalytic properties and/or stability would further extend its range of applications. Initial HRP mutagenesis studies set out to identify the critical residues involved in HRP catalysis [1].…”

Introductory Chapter: Multi-Aspect Bibliographic Analysis of the Synergy of Technical, Biological and Medical Sciences Concerning Materials and Technologies Used for Medical and Dental Implantable Devices

“…In the case of enzyme-mediated cross-linking [458], transglutaminases (including Factor Xllla) and horseradish peroxidases (HRP) [459] are used for the catalysis of star-shaped PEG hydrogels [496] and tissue transglutaminase catalysed PEG hydrogels [497]. This also applies tyrosinase, phosphopantetheinyl transferase, lysyl oxidase, plasma amine oxidase, and phosphatases [498].…”

Consensus mutagenesis reveals that non-helical regions influence thermal stability of horseradish peroxidase

“…1.11.17) are a ubiquitous subset of enzymes found throughout the animal and plant kingdoms [1]. The enzyme from horseradish roots (Armoracia rusticana; HRP) is the most widely studied example, due mainly to its many diverse uses in biotechnology [2]. Although moderately stable, the availability of a stabilized form of HRP would increase its applicability still further.…”