Horseradish Peroxidase as a Catalyst for Atom Transfer Radical Polymerization

Sigg, Severin J.; Seidi, Farzad; Renggli, Kasper; Silva, Tilana B.; Kali, Gergely; Bruns, Nico

doi:10.1002/marc.201100349

Cited by 132 publications

(89 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In parallel and independently, Bruns and co‐workers reported the ARGET‐ATRP of N ‐isopropylacrylamide in water at pH 6 and room temperature in the presence of HRP/ascorbate as the catalytic system and 2‐hydroxyethyl‐2‐bromoisobutyrate as the initiator 38. Although the experimental molecular weights were higher than the theoretical values, which indicates a low initiation efficiency under the conditions used, polydispersity indices decreasing with monomer conversion down to 1.44 were obtained.…”

Section: Naturally Occurring Transition‐metal Systemsmentioning

confidence: 99%

Transition‐Metal Catalysts for Controlled Radical Polymerization: A First Update

Fetzer¹,

Toniazzo²,

Ruch³

et al. 2012

Israel Journal of Chemistry

View full text Add to dashboard Cite

This article, a follow‐up on a recent review co‐authored by one of us (Prog. Polym. Sci. 2010, 35, 959–1021) discusses the most recent discoveries in the field of metal‐catalyzed, reversible‐deactivation radical polymerization. Remarkable examples include the use of non‐toxic polyethylene glycols simultaneously as solvents and ligands for the iron‐catalyzed ATRP, nickel complexes that promote polymerization via a dual radical/migratory‐insertion pathway, and metalloenzymes as mediators of ARGET ATRP‐type reactions. A thorough and critical overview of the mechanistic details as well as the experimental conditions used to carry out the polymerizations are given together with an overview of the future challenges.

show abstract

Section: Naturally Occurring Transition‐metal Systemsmentioning

confidence: 99%

Transition‐Metal Catalysts for Controlled Radical Polymerization: A First Update

Fetzer¹,

Toniazzo²,

Ruch³

et al. 2012

Israel Journal of Chemistry

View full text Add to dashboard Cite

show abstract

“…Biocatalytic reversible deactivation radical polymerization (RDRP) [1] techniques are of great interest for both polymer chemists and biologists. [2] Novel approaches to bioengineering are becoming increasingly valued, with significant growth forecast in cell-related technologies,s uch as cell separation and cell devices,or"biological machines".…”

mentioning

confidence: 99%

Blood‐Catalyzed RAFT Polymerization

et al. 2018

View full text Add to dashboard Cite

The use of hemoglobin (Hb) contained within red blood cells to drive a controlled radical polymerization via a reversible addition-fragmentation chain transfer (RAFT) process is reported for the first time. No pre-treatment of the Hb or cells was required prior to their use as polymerization catalysts, indicating the potential for synthetic engineering in complex biological microenvironments without the need for ex vivo techniques. Owing to the naturally occurring prevalence of the reagents employed in the catalytic system (Hb and hydrogen peroxide), this approach may facilitate the development of new strategies for in vivo cell engineering with synthetic macromolecules.

show abstract

“…However, since the CLRP methods generally require tedious pretreatment process prior to the immobilization of initiators on surface, it is difficult to directly apply them to inert polymeric substrates. What is worse, considering the restraints of bio-applications, i.e., the susceptibility of biomolecules to harsh environments, most of these systems have inherent adverse elements such as the copper used in atom transfer radical polymerization (ATRP)20, high temperatures in nitroxide-mediated polymerization (NMP)21, and UV irradiation in photoiniferter-controlled polymerization22. Therefore, the goal of developing a polymerization strategy capable of producing the desired molecular network but also compatible with biomolecules represents a great challenge.…”

mentioning

confidence: 99%

PEG Molecular Net-Cloth Grafted on Polymeric Substrates and Its Bio-Merits

Zhao

Lin

Yin

et al. 2014

Sci Rep

View full text Add to dashboard Cite

Polymer brushes and hydrogels are sensitive to the environment, which can cause uncontrolled variations on their performance. Herein, for the first time, we report a non-swelling “PEG molecular net-cloth” on a solid surface, fabricated using a novel “visible light induced surface controlled graft cross-linking polymerization” (VSCGCP) technique. Via this method, we show that 1) the 3D-network structure of the net-cloth can be precisely modulated and its thickness controlled; 2) the PEG net-cloth has excellent resistance to non-specific protein adsorption and cell adhesion; 3) the mild polymerization conditions (i.e. visible light and room temperature) provided an ideal tool for in situ encapsulation of delicate biomolecules such as enzymes; 4) the successive grafting of reactive three-dimensional patterns on the PEG net-cloth enables the creation of protein microarrays with high signal to noise ratio. Importantly, this strategy is applicable to any C-H containing surface, and can be easily tailored for a broad range of applications.

show abstract

Horseradish Peroxidase as a Catalyst for Atom Transfer Radical Polymerization

Cited by 132 publications

References 35 publications

Transition‐Metal Catalysts for Controlled Radical Polymerization: A First Update

Transition‐Metal Catalysts for Controlled Radical Polymerization: A First Update

Blood‐Catalyzed RAFT Polymerization

PEG Molecular Net-Cloth Grafted on Polymeric Substrates and Its Bio-Merits

Contact Info

Product

Resources

About