2020
DOI: 10.1089/ten.tec.2020.0083
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Horseradish Peroxidase-Catalyzed Crosslinking of Fibrin Microthread Scaffolds

Abstract: Horseradish peroxidase (HRP) has been investigated as a catalyst to crosslink tissue-engineered hydrogels because of its mild reaction conditions and ability to modulate the mechanical properties of the matrix. Here, we report the results of the first study investigating the use of HRP to crosslink fibrin scaffolds. We examined the effect of varying HRP and hydrogen peroxide (H 2 O 2 ) incorporation strategies on the resulting crosslink density and structural properties of fibrin in a microthread scaffold form… Show more

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Cited by 14 publications
(8 citation statements)
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“…Moreover, enzymatic crosslinking is highly tunable and cyto-compatible and therefore, has been used widely for many tissue engineering applications. 39,40…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…Moreover, enzymatic crosslinking is highly tunable and cyto-compatible and therefore, has been used widely for many tissue engineering applications. 39,40…”
Section: Resultsmentioning
confidence: 99%
“…Moreover, enzymatic crosslinking is highly tunable and cyto-compatible and therefore, has been used widely for many tissue engineering applications. 39,40 Although the presence of CMC in scaffolds is known to provide resilience, it lacks the ability to bind to growth factors. Therefore, to enable growth factor binding ability and its longterm presentation to cells, CMC was subjected to chemical sulfation to obtain sulfated-CMC (s-CMC) (Fig.…”
Section: Synthesis and Characterization Of Modified Polymersmentioning
confidence: 99%
See 1 more Smart Citation
“…Among several peroxidase types, horseradish peroxidase (HRP) isolated from Armoracia rusticana has been extensively employed in the fabrication of hydrogels. HRP can be isolated from renewable sources with affordable costs for extraction and purification processes [ 199 , 200 ]. However, the activity of HRP in the presence of hydrogen peroxide can yield reduced cell viability [ 201 ].…”
Section: Chemical and Non-chemical Cross-linking In Scaffold Synthesismentioning
confidence: 99%
“…This hypothesis is supported by the increase in denaturation temperature up to 59 1C in pure collagen hydrogel after photocrosslinking, indicating fibrils were stabilized possibly through new tyrosine/tyrosine covalent bonds. 27,28 The difference of collagen denaturation temperature between pure and collagen/THA hydrogels could be due to the formation of supplementary tyrosine/tyramine bounds.…”
Section: Tha Gelling By Enzymatic or Photocrosslinkingmentioning
confidence: 99%