Horticultural characterization of a tetraploid transgenic plant of &lt;i&gt;Tricyrtis&lt;/i&gt; sp. carrying the gibberellin 2-oxidase gene

Ôtani, Midori; Ishibe, Mitsuyo; Inthima, Phithak; Supaibulwatana, Kanyaratt; Mori, Satoshi; Niki, Tomoko; Nishijima, Takaaki; Koshioka, Masaji; Nakano, Masaru

doi:10.5511/plantbiotechnology.14.0916a

Cited by 6 publications

(4 citation statements)

References 30 publications

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“…Tetraploids of L. sinuatum have wide leaves, large stomata, low stomata density, and large flowers, and these morphological characteristics were consistent with those of polyploid L. bellidifolium [20]. In addition, leaf ovalization [10], stomatal enlargement [26][27][28], and flower enlargement [29][30][31][32][33][34] as a consequence of chromosome doubling has been reported in other ornamental plants. Thick stem wings and inflorescence clogging in the tetraploids obtained in this study are not desirable from an ornamental point of view.…”

Section: Discussionsupporting

confidence: 76%

Morphological Characterization of Tetraploids of Limonium sinuatum (L.) Mill. Produced by Oryzalin Treatment of Seeds

et al. 2021

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Limonium sinuatum (L.) Mill. (2n = 2x = 16) is a popular ornamental plant with dimorphism of pollen grains (type A and type B) and stigmas (papilla and cob-like). We applied polyploidy breeding to this species in order to introduce desirable traits. Tetraploid and mixoploid L. sinuatum plants were successfully obtained with oryzalin treatment of L. sinuatum ‘Early Blue’ seeds. All three tetraploids had increased leaf width, stomatal size, flower length, and pollen width compared to those of the diploid, and tetraploids had four germinal pores of pollen grains, whereas the diploid had three. All tetraploids had type A pollen grains and cob-like stigmas. Furthermore, the growth of cultivated tetraploid plants was slow, with later bolting and flowering times. Mixoploids Mixo-1 and Mixo-3 were estimated to be polyploidy periclinal chimeric plants consisting of a tetraploid L1 layer and diploid L2 layer, and Mixo-2 was estimated to be a polyploidy periclinal chimeric plant consisting of the diploid L1 layer and tetraploid L2 layer. Mixo-4 had tetraploid L1 and L2 layers. Mixoploids, except Mixo-4, had type A pollen grains and cob-like stigmas, whereas Mixo-4 had type B pollen grains and papilla stigmas. These polyploids will be useful as polyploidy breeding materials.

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Section: Discussionsupporting

confidence: 76%

Morphological Characterization of Tetraploids of Limonium sinuatum (L.) Mill. Produced by Oryzalin Treatment of Seeds

et al. 2021

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“…Increased stomatal size after chromosome doubling has been reported in some ornamentals, such as Anthurium andraeanum (Chen et al, 2011), Gerbera jamesonii (Gantait et al, 2011), and Tricyrtis sp. (Otani et al, 2014). In the present study, tetraploid L. bellidifolium had larger flowers than a control diploid plant (Table 3).…”

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confidence: 75%

Chromosome Doubling in <i>Limonium bellidifolium</i> (Gouan) Dumort. by Colchicine Treatment of Seeds

et al. 2016

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Limonium bellidifolium (2n = 2x = 18), a perennial statice belonging to the family Plumbaginaceae, is cultivated as a garden plant or for cut flowers and is an important breeding material for the production of hybrid cultivars in the genus. In this study, chromosome doubling in L. bellidifolium was attempted to increase the variability in horticultural traits. Seeds of this species were treated with an antimitotic agent, colchicine, at different concentrations and exposure periods. The treated seeds were sown in soil in a cell tray and the seedlings were grown in a greenhouse. More than 50% of the seedlings treated with colchicine for 24 or 48 h, irrespective of the concentration, survived for 4 months after treatment. Most of the seedlings treated for 72 h at any concentration showed very poor growth and abnormal thickening of the hypocotyl, and ultimately died. The surviving seedlings were grown in 9-cm pots. Flow cytometry analysis of leaf tissues showed that 2.5%-5% of plants that received 0.05% colchicine for 72 h, 0.25% colchicine for 24 h, 0.25% colchicine for 48 h, or 0.5% colchicine for 48 h were tetraploid (4x) or mixoploid (2x + 4x). The highest frequencies of tetraploids and mixoploids occurred following treatment with 0.05% colchicine for 72 h. These results showed that colchicine treatment of seeds is effective for chromosome doubling in L. bellidifolium. After 3 years of cultivation, the morphological characteristics of diploid and tetraploid L. bellidifolium plants at the flowering stage were investigated. The stomatal density was lower in all investigated tetraploid and mixoploid plants than in the control diploid plant. The stomatal length was 1.1-to 1.5-fold higher in all tetraploid and mixoploid plants than in the control. Tetraploid plants tended to have wider and thicker leaves than the control and also produced larger flowers.

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“…Transgenic Tricyrtis sp. plants with altered flower forms (Nakano et al 2007), altered flower colors (Kamiishi et al 2012), and altered plant form (Otani et al 2013(Otani et al , 2014a(Otani et al , 2014b) have already been produced. Wide hybrid plants have also been produced via ovule culture between transgenic and non-transgenic plants in Tricyrtis (Otani et al 2019).…”

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confidence: 99%

Plant regeneration and <i>Agrobacterium</i>-mediated genetic transformation systems in liliaceous ornamental plants

Nakano

Ôtani

2020

Plant Biotechnology

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The family Liliaceae (Cronquist system) contains various important ornamental plants. We have been examining for about 20 years the establishment of plant regeneration and genetic transformation systems in liliaceous ornamental plants for their biotechnological breeding and elucidation of the molecular mechanisms determining ornamental traits. In this review, studies on in vitro plant regeneration in 7 genera and on Agrobacterium-mediated production of transgenic plants in 4 genera are described. Plant regeneration was achieved via callus cultures in Agapanthus, Hemerocallis, Hosta, Lilium, Muscari and Tricyrtis. Auxins (2,4-dichrolophenoxyacetic acid, α-naphthaleneacetic acid and/or picloram) were effective for inducing regenerable calli. Tulipa species and cultivars were very recalcitrant to callus induction and plant regeneration. Agrobacterium-mediated transformation was examined in Agapanthus, Lilium, Muscari and Tricyrtis, and transgenic plants were obtained in all genera by using regenerable calli as a target material for Agrobacterium inoculation, inoculation and co-cultivation with Agrobacterium in the presence of acetosyringone, and selection of transgenic tissues and plantlets on hygromycin-containing media. Among 4 genera, Tricyrtis has several advantages for transformation studies: higher transformation efficiency, relatively small plant size, ease of cultivation, and taking only 1 year from in vitro regeneration to flowering. We are now investigating the molecular mechanisms for determining plant form, flower color and flower form by using Tricyrtis spp. as liliaceous model plants.

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Horticultural characterization of a tetraploid transgenic plant of <i>Tricyrtis</i> sp. carrying the gibberellin 2-oxidase gene

Cited by 6 publications

References 30 publications

Morphological Characterization of Tetraploids of Limonium sinuatum (L.) Mill. Produced by Oryzalin Treatment of Seeds

Morphological Characterization of Tetraploids of Limonium sinuatum (L.) Mill. Produced by Oryzalin Treatment of Seeds

Chromosome Doubling in <i>Limonium bellidifolium</i> (Gouan) Dumort. by Colchicine Treatment of Seeds

Plant regeneration and <i>Agrobacterium</i>-mediated genetic transformation systems in liliaceous ornamental plants

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