Hospital Isolates of
            <i>Serratia marcescens</i>
            Transferring Ampicillin, Carbenicillin, and Gentamicin Resistance to Other Gram-Negative Bacteria Including
            <i>Pseudomonas aeruginosa</i>

Olexy, Vera M.; Bird, Thomas J.; Grieble, Hans G.; Farrand, Stephen K.

doi:10.1128/aac.15.1.93

Cited by 27 publications

(26 citation statements)

References 18 publications

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“…Strains of Escherichia coli were maintained on Luria-Bertani medium (27), and other bacteria were kept on Nutrient Agar medium (Difco). The minimal medium used for E. coli (22) was supplemented when appropriate with 100 ,ug of thiamine per ml or 25 p,g of proline per ml. The minimal medium for Agrobacterium, Pseudomonas, and Rhizobium spp.…”

Section: Methodsmentioning

confidence: 99%

Spontaneous mutation conferring the ability to catabolize mannopine in Agrobacterium tumefaciens

et al. 1992

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Two nopaline-type strains ofAgrobacterium tumefaciens, C58 and T37, as well as strain A136, which is a Ti plasmid-cured derivative of strain C58, gave rise to spontaneous mutants that were able to grow on mannopine.The observation of mutagenesis with strain A136 demonstrated that the ability to acquire this new catabolic potential was independent of the presence of a Ti plasmid. The mutants were isolated after 4 weeks of incubation on minimal medium containing mannopine as the sole carbon source. They also utilized mannopinic acid, but not agropine or agropinic acid. In addition, the spontaneous mutant LM136, but not its parent strain A136, degraded many mannityl opine analogs.[14C]mannopine disappeared in the presence of LM136 cells which had been pregrown on opine or nonopine substrates. These results suggested that the catabolic system of this mutant was not subject to a stringent regulation. A clone conferring the ability to utilize mannopine on a recipient pseudomonad was selected from a genomic library from both the mutant LM136 and its parent strain. Only the LM136 clone was expressed in the parent Agrobacterium strain A136. Southern analysis showed that the genes for mannopine catabolism in the spontaneous mutants differed from the corresponding Ti plasmid-encoded genes of octopine-type or agropine-type Agrobacterium strains. Cells of LM136 utilized[14C]mannopine without generating detectable amounts of intracellular agropine. In contrast, a major fraction of the radioactivity recovered from cells of the octopine-type strain Ach5, after incubation on [14Cjmannopine, was in the form of agropine.Crown gall and hairy root are plant diseases caused by Agrobacterium tumefaciens and Agrobacterium rhizogenes, respectively. Pathogenic bacteria harbor either a Ti (tumorinducing) or a Ri (root-inducing) plasmid. The bacteria mediate the transfer to the plant cell of a portion of the plasmid, called transferred DNA (T-DNA). The T-DNA becomes incorporated into the plant nuclear DNA, where its expression results in abnormal patterns of phytohormone metabolism and proliferation of the transformed plant cells.

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Section: Methodsmentioning

confidence: 99%

Spontaneous mutation conferring the ability to catabolize mannopine in Agrobacterium tumefaciens

et al. 1992

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“…The R plasmids were transferred by the conjugation method (16). Matings of the original cultures (donors) with the recipient cultures were made by mixing 0.1 ml of a midexponential phase culture of the donor with 0.45 ml of a similar culture of the recipient in a test tube containing 0.45 ml of fresh brain heart infusion broth (BHI, Difco Laboratories, Detroit, Michigan), and the tubes were incubated with gentle shaking at 37 C for 3 hr.…”

Section: Methodsmentioning

confidence: 99%

A Possible Role of R Plasmids in Bacterial Permeability for β‐Lactam Antibiotics

Ikeuchi

Osada

1981

Microbiology and Immunology

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Four strains of clinical isolates of Serratia marcescens (13039, 13090, 13093, 14093) harboring R plasmids were highly resistant to ampicillin (ABPC) and cephaloridine (CER). With elimination of R plasmids from these strains by acriflavine treatment, ABPC-resistance levels of these strains were markedly reduced. Reduction of CER-resistance levels was also demonstrated in strains 13039 and 13093, but not in strains 13090 and 14093. The permeability of the former strains for CER was also decreased, but not in the latter strains. At the same time, fllactamase activity of these strains also almost completely disappeared when the R plasmids were eliminated. By broth matings with these strains, the recipient strains of S. marcescens 13031 (rif), Escherichia coli K-12 (rif), and E. coli 15046 (rif) all acquired a high permeability barrier against CER with inheritance of the R plasmids from strains 13039 and 13093, but not from strains 13090 and 14093. The transconjugant of strain 13031 that inherited R plasmid 13093 was resistant not only to CER but also to cefazolin, cephalothin, and cephalexin. Its permeabiiity to these antibiotics was significantly lower than that of the original strain. This fact suggests the possibility that the R plasmid from strain 13093 may be involved not only in production of p-lactamases, but also in regulation of bacterial permeability for cephalosporins.

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“…We also showed that pFMH1010 is conjugally transmissible from nosocomial Serratia marcescens isolates to the P. aeruginosa laboratory strain, PA038-1 (23,24). The resulting transconjugants gain resistance to carbenicillin, gentamicin, tobramycin, kanamycin, streptomycin, and sisomicin (23,24).…”

mentioning

confidence: 91%

“…The standard bacterial strains and plasmids used are shown in Table 1. Rifampinresistant (Rif) mutants of Escherichia coli 1231 and P. aeruginosa PA0227 were isolated as previously described (23).…”

Section: Methodsmentioning

confidence: 99%

Diversity of determinants encoding carbenicillin, gentamicin, and tobramycin resistance in nosocomial Pseudomonas aeruginosa

Mucha¹,

Farrand²

1986

Antimicrob Agents Chemother

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Plasmid pFMH1010, an 89-megadalton R plasmid, is endemic among members of the family Enterobacteriaceae at Hines Veterans Administration Hospital, Hines, Ill. It encodes resistance to nine antibiotics, including resistance to carbenicillin (Cb), gentamicin (Gm), and tobramycin (Tm). Pseudomonas aeruginosa strains resistant to carbenicillin, gentamicin, and tobramycin were isolated from five patients at Hines Veterans Administration Hospital from whom Serratia marcescens strains harboring pFMH1010 were also obtained. The P. aeruginosa strains were investigated to determine whether their Cb, Gm, and Tm characteristics derived from pFMH1010. One of the isolates, Ps559, was shown by Southern hybridization to contain approximately 76% of pFMH1010. Several lines of evidence suggested that the pFMH1010 sequences in Ps559 are integrated in the chromosome. Southern hybridization also demonstrated that the beta-lactam resistance of pFMH1010 is most probably due to the presence of sequences homologous with Tn3 and that these sequences are retained in Ps559. In two other Pseudomonas isolates, resistance to carbenicillin, gentamicin, tobramycin, and kanamycin was encoded by R plasmids unrelated to pFMH1010. In the last two isolates, resistance to gentamicin and tobramycin and several other antibiotics appeared to be chromosomally encoded, and it was rescuable from one of these strains by RP4-mediated mobilization.

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Hospital Isolates of Serratia marcescens Transferring Ampicillin, Carbenicillin, and Gentamicin Resistance to Other Gram-Negative Bacteria Including Pseudomonas aeruginosa

Cited by 27 publications

References 18 publications

Spontaneous mutation conferring the ability to catabolize mannopine in Agrobacterium tumefaciens

Spontaneous mutation conferring the ability to catabolize mannopine in Agrobacterium tumefaciens

A Possible Role of R Plasmids in Bacterial Permeability for β‐Lactam Antibiotics

Diversity of determinants encoding carbenicillin, gentamicin, and tobramycin resistance in nosocomial Pseudomonas aeruginosa

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