Hospital Trichosporon asahii isolates with simple architecture biofilms and high resistance to antifungals routinely used in clinical practice

Malacrida, Amanda Milene; Corrêa, Jakeline Luiz; Barros, Isabella Letícia Esteves; Veiga, Flávia Franco; Pereira, Elton da Cruz Alves; Negri, Melyssa; Svidzinski, Terezinha Inez Estivalet

doi:10.1016/j.mycmed.2022.101356

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…Amphotericin B and fluconazole are used to treat T. asahii infections 45 , 46 . The hog1 gene-deficient mutants of C. neoformans and C. albicans are sensitive to an antifungal agent the amphotericin B (AMPH-B) 19 , 23 , 38 , 42 .…”

Section: Resultsmentioning

confidence: 99%

Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii

Matsumoto,

Sugiyama,

Nagamachi

et al. 2023

Sci Rep

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Trichosporon asahii is an opportunistic pathogenic fungus that causes severe and sometimes fatal infections in immunocompromised patients. Hog1, a mitogen-activated protein kinase, regulates the stress resistance of some pathogenic fungi, however its role in T. asahii has not been investigated. Here, we demonstrated that the hog1 gene-deficient T. asahii mutant is sensitive to high temperature, cell membrane stress, oxidative stress, and antifungal drugs. Growth of the hog1 gene-deficient T. asahii mutant was delayed at 40 °C. The hog1 gene-deficient T. asahii mutant also exhibited sensitivity to sodium dodecyl sulfate, hydrogen peroxide, menadione, methyl methanesulfonate, UV exposure, and antifungal drugs such as amphotericin B under a glucose-rich condition. Under a glucose-restricted condition, the hog1 gene-deficient mutant exhibited sensitivity to NaCl and KCl. The virulence of the hog1 gene-deficient mutant against silkworms was attenuated. Moreover, the viability of the hog1 gene-deficient mutant decreased in the silkworm hemolymph. These phenotypes were restored by re-introducing the hog1 gene into the gene-deficient mutant. Our findings suggest that Hog1 plays a critical role in regulating cellular stress responses in T. asahii.

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Section: Resultsmentioning

confidence: 99%

Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii

Matsumoto,

Sugiyama,

Nagamachi

et al. 2023

Sci Rep

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“…However, in recent years, there have been increasing reports of azole resistance in clinical isolates of T. asahii and therapeutic failure after azole application. A number of clinical isolates have been found to be insensitive to fluconazole in in vitro drug sensitivity tests, resulting in a poor response to antifungal therapy in patients with invasive trichosporonosis [7,8].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of the Medium-Chain Dehydrogenase/Reductase Superfamily of Trichosporon asahii and Its Involvement in the Regulation of Fluconazole Resistance

Ma,

Liu,

Zeng

et al. 2024

JoF

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The medium-chain dehydrogenase/reductase (MDR) superfamily contains many members that are widely present in organisms and play important roles in growth, metabolism, and stress resistance but have not been studied in Trichosporon asahii. In this study, bioinformatics and RNA sequencing methods were used to analyze the MDR superfamily of T. asahii and its regulatory effect on fluconazole resistance. A phylogenetic tree was constructed using Saccharomyces cerevisiae, Candida albicans, Cryptococcus neoformans, and T. asahii, and 73 MDRs were identified, all of which contained NADPH-binding motifs. T. asahii contained 20 MDRs that were unevenly distributed across six chromosomes. T. asahii MDRs (TaMDRs) had similar 3D structures but varied greatly in their genetic evolution at different phylum levels. RNA-seq and gene expression analyses revealed that the fluconazole-resistant T. asahii strain upregulates xylitol dehydrogenase, and downregulated alcohol dehydrogenase and sorbitol dehydrogenase concluded that the fluconazole-resistant T. asahii strain was less selective toward carbon sources and had higher adaptability to the environment. Overall, our study contributes to our understanding of TaMDRs, providing a basis for further analysis of the genes associated with drug resistance in T. asahii.

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Genetic analysis of emerging fungal pathogens: Trichosporon asahii

Pumeesat,

Wongsuk

2023

Diagnostic Microbiology and Infectious Disease

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Hospital Trichosporon asahii isolates with simple architecture biofilms and high resistance to antifungals routinely used in clinical practice

Cited by 4 publications

References 33 publications

Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii

Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii

Genome-Wide Identification and Characterization of the Medium-Chain Dehydrogenase/Reductase Superfamily of Trichosporon asahii and Its Involvement in the Regulation of Fluconazole Resistance

Genetic analysis of emerging fungal pathogens: Trichosporon asahii

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