not show reduced glucose consumption when exposed to increased glucose concentrations for up to 6 days [1], in contrast to rat and bovine arterial smooth muscle cells exposed to high glucose concentrations for 24 h [7±8]. Accordingly, human arterial smooth muscle cells incubated in the presence of 15 mmol/l glucose reduce glucose concentrations to near normal (4.8 0.97 mmol/l glucose) in 3 days (Fig. 1).In conclusion, our observations show that normal human aortic smooth muscle cells in culture consume large amounts of glucose and, as a result, these cells rapidly deplete cell culture media of glucose. The results further indicate the advantage of monitoring cell glucose consumption in studies that aim to compare the effects of normal compared with high glucose concentrations on different processes in human arterial smooth muscle cells and other types of cells that could share similar characteristics.