Host Age Prediction from Fecal Microbiota Composition in Male C57BL/6J Mice

Low, Adrian; Soh, Melissa; Miyake, Sou; Seedorf, Henning

doi:10.1128/spectrum.00735-22

Cited by 18 publications

(10 citation statements)

References 60 publications

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“…Further, regardless of sex, aged mice had lower relative abundance of A. municiphila, an important microbe known to be associated with gut health (Xu et al, 2020). In support of these findings, a recent publication in C57BL/6J males across lifespan was also shown to have an age-associated loss of A. municiphila (Low et al, 2022). Our results demonstrate many differences in the microbiome were also associated sex, with more pronounced sex-specific differences in the aged population.…”

Section: Discussionsupporting

confidence: 85%

Modulation of microbiome diversity and cytokine expression is influenced in a sex-dependent manner during aging

Webster¹,

Vos²,

Rothstein³

et al. 2022

Front. Microbiom.

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The microbiome and immune system have a unique interplay, which influences homeostasis within the organism. Both the microbiome and immune system play important roles in health and diseases of the aged including development of cancer, autoimmune disorders, and susceptibility to infection. Various groups have demonstrated divergent changes in the gut microbiota during aging, yet the compounding factor of biological sex within the context of aging remains incompletely understood, and little is known about the effect of housing location in the composition of gut microbiota in the context of both sex and age. To better understand the roles of sex, aging, and location in influencing the gut microbiome, we obtained normal healthy BALB/cByJ mice from a single source and aged male and female mice in two different geographical locations. The 16S rRNA was analyzed from fecal samples of these mice and cytokine levels were measured from serum. 16S rRNA microbiome analysis indicated that both age and sex play a role in microbiome composition, whereas location plays a lesser role in the diversity present. Interestingly, microbiome changes occurred with alterations in serum expression of several different cytokines including IL-10 and IL-6, which were also both differentially regulated in context to sex and aging. We found both IL-10 and IL-6 play a role in the constitutive expression of pSTAT-3 in CD5+ B-1 cells, which are known to regulate the microbiome. Additionally, significant correlations were found between cytokine expression and significantly abundant microbes. Based on these results, we conclude aging mice undergo sex-associated alterations in the gut microbiome and have a distinct cytokine profile. Further, there is significant interplay between B-1 cells and the microbiome which is influenced by aging in a sex-dependent manner. Together, these results illustrate the complex interrelationship among sex, aging, immunity, housing location, and the gut microbiome.

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Section: Discussionsupporting

confidence: 85%

Modulation of microbiome diversity and cytokine expression is influenced in a sex-dependent manner during aging

Webster¹,

Vos²,

Rothstein³

et al. 2022

Front. Microbiom.

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“…We showed that Tmu induces longitudinal shifts in bacterial composition and increases abundance and activity of Helicobacter spp., consistent with previous trichomonad studies [ 15 , 80 ]. Although previous studies have shown natural microbiome variation in mice over their lifespan [ 81 ], our metatranscriptomic data in particular suggest little change over the 28 days of the experiment. As mice were co-housed prior to protist exposure to normalize their microbiomes, the similarity of 16S rRNA compositions in day 28 control mice to day 0 preinfection samples, relative to the large Tmu -driven shift in the colonized mice, further supports limited variation during this period.…”

Section: Discussioncontrasting

confidence: 66%

Commensal protistTritrichomonas musculusexhibits a dynamic life cycle that induces extensive remodeling of the gut microbiota

Popovic,

Cao,

Han

et al. 2024

The ISME Journal

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Commensal protists and gut bacterial communities exhibit complex relationships, mediated at least in part through host immunity. To improve our understanding of this tripartite interplay, we investigated community and functional dynamics between the murine protist Tritrichomonas musculus (Tmu) and intestinal bacteria in healthy and B cell-deficient mice. We identified dramatic, protist-driven remodeling of resident microbiome growth and activities, in parallel with Tmu functional changes, which were accelerated in the absence of B cells. Metatranscriptomic data revealed nutrient-based competition between bacteria and the protist. Single cell transcriptomics identified distinct Tmu life stages, providing new evidence for trichomonad sexual replication and the formation of pseudocysts. Unique cell states were validated in situ through microscopy and flow cytometry. Our results reveal complex microbial dynamics during the establishment of a commensal protist in the gut, and provide valuable datasets to drive future mechanistic studies.

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“…We found no significant separation in the laryngeal microbiota (i.e., no significant differences in post-hoc analyses of beta-diversity) across age groups. Although the gut microbiota has been shown to vary throughout the lifespan [ 52 ], similar result has not been reported in the laryngeal microbiome. Lastly, while the selection of age group is based on prior research [ 27 ], it is possible that the age groups may not accurately reflect microbial variation across aging.…”

Section: Discussionmentioning

confidence: 79%

Age and sex-related variations in murine laryngeal microbiota

An,

Venkatraman,

Binns

et al. 2024

PLoS ONE

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The larynx undergoes significant age and sex-related changes in structure and function across the lifespan. Emerging evidence suggests that laryngeal microbiota influences immunological processes. Thus, there is a critical need to delineate microbial mechanisms that may underlie laryngeal physiological and immunological changes. As a first step, the present study explored potential age and sex-related changes in the laryngeal microbiota across the lifespan in a murine model. We compared laryngeal microbial profiles of mice across the lifespan (adolescents, young adults, older adults and elderly) to determine age and sex-related microbial variation on 16s rRNA gene sequencing. Measures of alpha diversity and beta diversity were obtained, along with differentially abundant taxa across age groups and biological sexes. There was relative stability of the laryngeal microbiota within each age group and no significant bacterial compositional shift in the laryngeal microbiome across the lifespan. There was an abundance of short-chain fatty acid producing bacteria in the adolescent group, unique to the laryngeal microbiota; taxonomic changes in the elderly resembled that of the aged gut microbiome. There were no significant changes in the laryngeal microbiota relating to biological sex. This is the first study to report age and sex-related variation in laryngeal microbiota. This data lays the groundwork for defining how age-related microbial mechanisms may govern laryngeal health and disease. Bacterial compositional changes, as a result of environmental or systemic stimuli, may not only be indicative of laryngeal-specific metabolic and immunoregulatory processes, but may precede structural and functional age-related changes in laryngeal physiology.

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Host Age Prediction from Fecal Microbiota Composition in Male C57BL/6J Mice

Cited by 18 publications

References 60 publications

Modulation of microbiome diversity and cytokine expression is influenced in a sex-dependent manner during aging

Modulation of microbiome diversity and cytokine expression is influenced in a sex-dependent manner during aging

Commensal protistTritrichomonas musculusexhibits a dynamic life cycle that induces extensive remodeling of the gut microbiota

Age and sex-related variations in murine laryngeal microbiota

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