Host and Nonhost Effects on Soil Populations of Phytophthora parasitica var. nicotianae

Flowers, R. A.

doi:10.1094/phyto-64-718

Cited by 3 publications

(2 citation statements)

References 2 publications

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“…The sporulation of M. anisopliae s.l. on OA-CTAB 6 is an important factor because early sporulation of target fungi allows their rapid identification (Flowers and Hendrix 1969).…”

Section: Discussionmentioning

confidence: 99%

A novel dodine-free selective medium based on the use of cetyl trimethyl ammonium bromide (CTAB) to isolate Beauveria bassiana, Metarhizium anisopliae sensu lato and Paecilomyces lilacinus from soil

Posadas

Comerio²,

Mini³

et al. 2012

Mycologia

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This study evaluated the quaternary ammonium compound cetyl trimethyl ammonium bromide (CTAB) as an alternative to the chemically related dodecylguanidine (dodine) for the selective isolation of entomopathogenic fungi. Oatmeal agar (OA) with chloramphenicol was used as basal medium, and three concentrations of CTAB (0.5, 0.6, 0.7 g/L) were evaluated and compared against OA + 0.46 g/L dodine. Selective isolation and growth studies were performed with the entomopathogens Beauveria bassiana, Metarhizium anisopliae s.l. and Paecilomyces lilacinus and five common non-entomopathogenic non-target species. The three entomopathogenic fungi sporulated earlier on OA + 0.6 g/L CTAB than on OA + 0.46 g/L dodine, while none of the non-target fungi sporulated on OA + 0.6 g/L CTAB. All entomopathogenic fungal isolates grew on OA + 0.6 g/L CTAB, despite some intra-species variation, whereas non-target fungi showed no growth or sporulation. OA + 0.6 g/L CTAB resulted in an efficient medium to isolate B. bassiana, M. anisopliae s. l. and P. lilacinus from soil samples. Results of our study suggest that OA + 0.6 g/L CTAB is a suitable, simple and inexpensive to prepare medium to replace OA + 0.46 g/L dodine for the selective isolation of these fungi.

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“…The sporulation of M. anisopliae s.l. on OA-CTAB 6 is an important factor because early sporulation of target fungi allows their rapid identification (Flowers and Hendrix 1969).…”

Section: Discussionmentioning

confidence: 99%

A novel dodine-free selective medium based on the use of cetyl trimethyl ammonium bromide (CTAB) to isolate Beauveria bassiana, Metarhizium anisopliae sensu lato and Paecilomyces lilacinus from soil

Posadas

Comerio²,

Mini³

et al. 2012

Mycologia

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show abstract

“…nicotianae All of the susceptible Hicks and 58-61$ of the moderately resistant Speight G-28 plants died in three separate fields in north Florida which had initial populations of P. parasitica var. nicotianae of 0.2, 0.4, and 0.75 propagule per gram of soil(Mitchell, unpublished ) .Flowers and Hendrix (11,12) also reported severe disease under greenhouse or field conditions where initial populations of F. parasitica var.nicotianae were low. Kannwischer and itchell( unpublished ) found that approximately 0.2 chlamydospore per gram of soil was required for 5°?= infection of tobacco seedlings by P.parasitica var.…”

mentioning

confidence: 99%

Quantitative studies on the development and control of black shank of tobacco, and on the survival of Phytophthora parasitica var. Nicotianae in soil /

Kannwischer¹

1979

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Propagule size: Its relation to population density of microorganisms in soil

Chuang

1981

Soil Biology and Biochemistry

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Host and Nonhost Effects on Soil Populations of Phytophthora parasitica var. nicotianae

Cited by 3 publications

References 2 publications

A novel dodine-free selective medium based on the use of cetyl trimethyl ammonium bromide (CTAB) to isolate Beauveria bassiana, Metarhizium anisopliae sensu lato and Paecilomyces lilacinus from soil

A novel dodine-free selective medium based on the use of cetyl trimethyl ammonium bromide (CTAB) to isolate Beauveria bassiana, Metarhizium anisopliae sensu lato and Paecilomyces lilacinus from soil

Quantitative studies on the development and control of black shank of tobacco, and on the survival of Phytophthora parasitica var. Nicotianae in soil /

Propagule size: Its relation to population density of microorganisms in soil

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