Host and Parasite Factors Affecting the Invasion of Mononuclear Phagocytes by Trypanosoma cruzi

A Brief View of the Surface Membrane Proteins fromTrypanosoma cruzi

“…On the other hand, GP90 is a metacyclic stage-specific glycoprotein defined by its reactivity with monoclonal antibodies 1G7 and 5E7 [46]. GP90 expressed by metacyclic forms lacks any enzymatic activity [47]. GP90 is also present in the mammalian stages of T. cruzi (bloodstream trypomastigote and amastigotes stages) and has the antiphagocytic effect mediated by the removal of sugar residues necessary for parasite internalization.…”

“…GP90 is also present in the mammalian stages of T. cruzi (bloodstream trypomastigote and amastigotes stages) and has the antiphagocytic effect mediated by the removal of sugar residues necessary for parasite internalization. This surface glycoprotein appears to have glycosidase activity and downregulates host-cell invasion probably due to the fact that GP90 binds to mammalian cells in a receptor-mediated manner without triggering the Ca 2+ signal-inducing activity [10, 47]. …”

Review onTrypanosoma cruzi: Host Cell Interaction

“…In 1983, Nogueira [19] described, using macrophages as the host cell, the antiphagocytic effect of a gp90 molecule that is present in the mammalian stages of T. cruzi. This molecule seemed to have glycosidase activity and its antiphagocytic activity was mediated by the removal of sugar residues necessary for parasite internalization.…”

O efeito do selênio em ratas Wistar prenhas infectadas pela cepa Y de Trypanosoma cruzi

“…Esta molécula parece ter atividade semelhante a glicosidase e sua atividade antifagocitária é mediada pela remoção de resíduos de açúcar, necessários para a internalização do parasita (DE SOUZA et al, 2010;NOGUEIRA, 1983). Moléculas conhecidas como "mucinas" (glicoproteína presente em maior quantidade na superfície de T. cruzi) estão relacionadas com a ligação do parasita e a invasão (DE SOUZA et al, 2010;VILLALTA et al, 1984;YOSHIDA et al, 1989), assim como as moléculas da família Tc85, um tipo de trans-sialidase (única enzima na superfície de T. cruzi que facilita a invasão), gp83 (presente em todas as linhagens do parasita e responsável pelo ataque e entrada do protozoário em células fagocíticas e não fagocíticas), e a penetrina (que tem afinidade por matriz celular) (DE SOUZA et al, 2010;VILLALTA et al, 2008;LIMA et al, 1988).…”