Host-Associated Rhizobial Fitness: Dependence on Nitrogen, Density, Community Complexity, and Legume Genotype

Burghardt, Liana T.; Epstein, Brendan; Hoge, Michelle; Trujillo, Diana; Tiffin, Peter; Glass, ed.

doi:10.1128/aem.00526-22

Cited by 13 publications

(18 citation statements)

References 96 publications

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“…Fitness responses of white clover and rhizobia were unrelated to Rhizobium abundance in the root endosphere. As only R. leguminosarum can initiate nodulation on white clover (Andrews & Andrews, 2017), our results suggest that nodulation depended on having the ‘right’ Rhizobium partner (i.e., genotype), which is consistent with the findings from other studies (Burghardt et al, 2022; Heath, 2010; Heath & Tiffin, 2007; Van Cauwenberghe et al, 2016). We found no relationships between either microbiome inoculant dissimilarity or root endosphere composition and local adaptation, indicating that the broader bacterial microbiome did not affect local adaptation.…”

Section: Discussionsupporting

confidence: 92%

“…These findings contrast with recent studies that demonstrated adaptation to local microbiomes increases fitness (O’Brien et al, 2020; O’Brien, Yu, et al, 2022), and that dissimilarity between local and nonlocal inoculants modulates fitness responses (O’Brien, Laurich, et al, 2022). Importantly, rhizobia density in inoculants could also confound nodulation (Batstone et al, 2020; Burghardt et al, 2022), but our microbiome inoculants had similar densities of Rhizobium (Figure S10).…”

Section: Discussionmentioning

confidence: 99%

“…Alternatively, the absence of a correlation can arise despite ongoing coevolution because traits could be mismatched (Gomulkiewicz et al, 2007; Nuismer et al, 2010; Thompson, 2005). Further investigation of potential coevolution between white clover and rhizobia in urban environments requires characterizing selection acting on both white clover and rhizobia, and ideally genomic signatures of selection on genes associated with the mutualism (Batstone, Burghardt, et al, 2022; Batstone, Lindgren, et al, 2022; Epstein et al, 2023; Gomulkiewicz et al, 2007; Hoeksema, 2010).…”

Section: Discussionmentioning

confidence: 99%

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Mosaic of local adaptation between white clover and rhizobia along an urbanization gradient

Murray-Stoker,

Johnson

2023

Preprint

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Urbanization alters many biotic and abiotic environmental factors, but the effects of urbanization on local adaptation and coevolution between species remains largely unstudied. Using a common garden experiment of 30 populations and 1080 plants, we tested for local adaptation in the mutualism between white clover (Trifolium repens) and rhizobia (Rhizobium leguminosarum symbiovar trifolii) along an urbanization gradient. Our results show a spatial mosaic in local adaptation, with stronger local adaptation for rhizobia than white clover. While nitrogen addition (N) strongly influenced plant biomass and nodule density, it did not consistently mediate patterns of local adaptation. The strength of local adaptation was positively related between white clover and rhizobia under N addition, suggesting that soil N mediates coadaptation between white clover and rhizobia. Local adaptation was not influenced by urbanization, indicating that while urbanization does influence the ecology of plant-microbe interactions, it does not disrupt local adaptation and coevolution among mutualists.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Mosaic of local adaptation between white clover and rhizobia along an urbanization gradient

Murray-Stoker,

Johnson

2023

Preprint

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“…As only R. leguminosarum can initiate nodulation on white clover(Andrews & Andrews, 2017), our results suggest that nodulation depended on having the 'right' Rhizobium partner (i.e. genotype), which is consistent with the findings from other studies(Burghardt et al, 2022;Heath, 2010;Heath & Tiffin, 2007;Van Cauwenberghe et al, 2016).…”

supporting

confidence: 92%

“…et al, 2024). Importantly, rhizobia density in inoculants could also confound nodulation (Batstone et al, 2020;Burghardt et al, 2022), but our microbiome inoculants had similar densities of Rhizobium (Figure S10).…”

Section: Urbanization and Local Adaptationmentioning

confidence: 99%

Mosaic of local adaptation between white clover and rhizobia along an urbanization gradient

Murray‐Stoker,

Johnson

2024

Journal of Ecology

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Urbanization frequently alters the biotic and abiotic environmental context. Variation in both the biotic and abiotic environment can result in selection mosaics that lead to locally adapted phenotypes. Ecological changes to urban environments can result in evolutionary responses across a diverse array of plants and animals, but the effects of urbanization on local adaptation and coevolution between species remain largely unstudied. Using a common garden experiment of 30 populations and 1080 plants, we tested for local adaptation in the mutualism between white clover (Trifolium repens) and rhizobia (Rhizobium leguminosarum symbiovar trifolii) along an urbanization gradient. We asked: (Q1) are white clover and rhizobia locally adapted? (Q2) Does nitrogen (N) addition mediate the strength of local adaptation? (Q3) Is the strength of local adaptation in host plants related to the strength of local adaptation in rhizobia, as expected if white clover and rhizobia are coadapting? And (Q4) how does the degree of local adaptation vary with urbanization? We found evidence for local adaptation for both white clover and rhizobia, with stronger signatures of local adaptation for rhizobia than white clover (Q1). While N addition positively affected plant biomass and negatively affected nodule density, N addition did not consistently mediate patterns of local adaptation (Q2). The strength of local adaptation was positively related between white clover and rhizobia under N addition, suggesting that soil N mediates coadaptation between white clover and rhizobia (Q3). We did not find a consistent relationship between measures of urbanization and local adaptation (Q4). Additionally, fitness responses for white clover and rhizobia were unrelated to Rhizobium abundance and local adaptation was unrelated to the broader bacterial microbiome in the soil inoculants or root endosphere. Synthesis. Our results show a spatial mosaic in local adaptation, with stronger local adaptation for rhizobia than white clover. While urbanization does influence the ecology of plant–microbe interactions, our study suggests urbanization does not disrupt local adaptation and coevolution among mutualists.

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The evolutionary ecology of rhizobia: multiple facets of competition before, during, and after symbiosis with legumes

Burghardt

diCenzo

2023

Current Opinion in Microbiology

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Host-Associated Rhizobial Fitness: Dependence on Nitrogen, Density, Community Complexity, and Legume Genotype

Cited by 13 publications

References 96 publications

Mosaic of local adaptation between white clover and rhizobia along an urbanization gradient

Mosaic of local adaptation between white clover and rhizobia along an urbanization gradient

Mosaic of local adaptation between white clover and rhizobia along an urbanization gradient

The evolutionary ecology of rhizobia: multiple facets of competition before, during, and after symbiosis with legumes

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