Host-delivered RNAi-mediated silencing of the root-knot nematode (Meloidogyne incognita) effector genes, Mi-msp10 and Mi-msp23, confers resistance in Arabidopsis and impairs reproductive ability of the root-knot nematode

Kumar, Anil; Joshi, Ila; Changwal, Chunoti; Sirohi, Anil; Jain, Pradeep

doi:10.1007/s00425-022-03977-1

Cited by 6 publications

(3 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results exhibited the absence of motif 3 in M. chitwoodi and motif 5 in all species except M. incognita and M. javanica which suggests probable functional diversification of 16D10 in these species. One of the possible explanations of this divergence could be an adaptation to different host ranges or immune responses, allowing these nematodes to optimize their parasitic strategies within specific hosts (Khan et al, 2023;Kumar et al, 2022). The results of molecular docking of 16D10 peptides and Mi protein showed the top-ranking conformation for each peptide, that was identified by the lowest S score.…”

Section: Discussionmentioning

confidence: 99%

Computational analysis of 16D10 effector peptides from root-knot nematodes and their interaction with Mi resistance (R) protein through molecular docking

Zahoor,

Niaz,

Tahir-ul-Qamar

et al. 2024

Integr. Plant Biotechnol.

View full text Add to dashboard Cite

Plant-parasitic nematodes, especially root-knot nematodes (RKNs), pose significant threats to global agriculture, affecting both the quantity and quality of crop yields. Central to the parasitism strategy of RKNs is the deployment of effector proteins, such as the 16D10 peptide, which interact with host plant resistance mechanisms. This study focuses on the computational analysis and molecular docking of 16D10 effector peptides derived from major RKN species (M. incognita, M. arenaria, M. hapla, M. javanica, and M. chitwoodi) to explore their interaction with the Mi resistance protein in tomatoes. Initial bioinformatics assessments included sequence retrieval, multiple sequence alignment, and phylogenetic analysis, utilizing tools such as CLUSTALW and the MEME Suite. Notably, our phylogenetic analysis revealed a close evolutionary relationship between M. incognita and M. chitwoodi, contrasting with other RKN species based on both DNA and protein data. Subsequent, in silico 3D modeling and docking studies, conducted using Chem-Sketch and MOE software, provided insights into the structural basis of the interaction between 16D10 peptides and the Mi protein. Our findings indicate that these interactions could play a crucial role in the modulation of plant defense mechanisms, potentially leading to effector-triggered susceptibility (ETS). This study not only enhances our understanding of nematode-host interactions but also aids in the development of novel strategies for managing RKN infections in economically important crops.

show abstract

Section: Discussionmentioning

confidence: 99%

Computational analysis of 16D10 effector peptides from root-knot nematodes and their interaction with Mi resistance (R) protein through molecular docking

Zahoor,

Niaz,

Tahir-ul-Qamar

et al. 2024

Integr. Plant Biotechnol.

View full text Add to dashboard Cite

show abstract

“…On the other hand, double-stranded RNA (siRNA) activated the homologous mRNAs to inhibit their translation and transcription to silence genes. When siRNAs were injected into the nematode’s body, certain target genes became inactive [ 40 ]. This might be an effective way to delay pine wilt disease by targeting the silencing of pathogenic genes in nematodes.…”

Section: Discussionmentioning

confidence: 99%

Studies on the Requirement of Transthyretin Protein (BxTTR-52) for the Suppression of Host Innate Immunity in Bursaphelenchus xylophilus

Wen

Zhang

et al. 2022

IJMS

View full text Add to dashboard Cite

The pinewood nematode, Bursaphelenchus xylophilus, has been determined as one of the world’s top ten plant-parasitic nematodes. It causes pine wilt, a progressive disease that affects the economy and ecologically sustainable development in East Asia. B. xylophilus secretes pathogenic proteins into host plant tissues to promote infection. However, little is known about the interaction between B. xylophilus and pines. Previous studies reported transthyretin proteins in some species and their strong correlation with immune evasion, which has also been poorly studied in B. xylophilus. In this study, we cloned and functionally validated the B. xylophilus pathogenic protein BxTTR-52, containing a transthyretin domain. An in situ hybridization assay demonstrated that BxTTR-52 was expressed mainly in the esophageal glands of B. xylophilus. Confocal microscopy revealed that BxTTR-52-RFP localized to the nucleus, cytoplasm, and plasma membrane. BxTTR-52 recombinant proteins produced by Escherichia coli could be suppressed by hydrogen peroxide and antioxidant enzymes in pines. Moreover, silencing BxTTR-52 significantly attenuated the morbidity of Pinus thunbergii infected with B. xylophilus. It also suppressed the expression of pathogenesis-related genes in P. thunbergii. These results suggest that BxTTR-52 suppresses the plant immune response in the host pines and might contribute to the pathogenicity of B. xylophilus in the early infection stages.

show abstract

“…To assess the nematode infection in both control and WRKY-overexpressing transgenic lines, root samples were collected from the monoxenic culture after 28 days post-infection (dpi). The degree of nematode infection was determined by counting the number of galls and eggs per gram of root weight, following the methodology described in previous studies [54][55][56][57] . The average values of galls and eggs were obtained by analyzing 10-15 replicates per line.…”

Section: Evaluation Of Tomato Overexpression Roots In Response To Nem...mentioning

confidence: 99%

SlWRKY16 and SlWRKY31 of tomato, negative regulators of plant defense, involved in susceptibility activation following root-knot nematode Meloidogyne javanica infection

Kumar,

Sichov,

Bucki

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

The involvement of WRKY transcription factors in plant-nematode interactions, and in particular, how these WRKYs participate in regulating the complex morphological and physiological changes occurring after nematode infection, are the topic of active research. We characterized the functional role of the unstudied tomato WRKY genes SlWRKY16 and SlWRKY31 in regulating tomato roots’ response to infection by the root-knot nematode Meloidogyne javanica. Using promoter–GUS reporter gene fusions and qRT-PCR, we show that both SlWRKYs are predominantly expressed during the first half of the parasitic life stages, when feeding-site induction and construction occur. Expression of SlWRKY16 increased sharply 15 days after inoculation, whereas SlWRKY31 was already induced earlier, but reached its maximum expression at this time. Both genes were downregulated at the mature female stage. To determine biological function, we produced transgenic lines overexpressing SlWRKY16 and SlWRKY31 in tomato hairy roots. Overexpression of both genes resulted in enhanced M. javanica infection, reflected by increased galling occurrence and reproduction. Expression profiling of marker genes responsive to defense-associated phytohormones indicated reductions in salicylic acid defense-related PR-1 and jasmonic acid defense-related PI in inoculated roots overexpressing SlWRK16 and SlWRKY31, respectively. Our results suggest that SlWRKY16 and SlWRKY31 function as negative regulators of plant immunity induced upon nematode infection.

show abstract

Host-delivered RNAi-mediated silencing of the root-knot nematode (Meloidogyne incognita) effector genes, Mi-msp10 and Mi-msp23, confers resistance in Arabidopsis and impairs reproductive ability of the root-knot nematode

Cited by 6 publications

References 80 publications

Computational analysis of 16D10 effector peptides from root-knot nematodes and their interaction with Mi resistance (R) protein through molecular docking

Computational analysis of 16D10 effector peptides from root-knot nematodes and their interaction with Mi resistance (R) protein through molecular docking

Studies on the Requirement of Transthyretin Protein (BxTTR-52) for the Suppression of Host Innate Immunity in Bursaphelenchus xylophilus

SlWRKY16 and SlWRKY31 of tomato, negative regulators of plant defense, involved in susceptibility activation following root-knot nematode Meloidogyne javanica infection

Contact Info

Product

Resources

About