2023
DOI: 10.3390/antibiotics12020257
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Host Dependent-Transposon for a Plasmid Found in Aeromonas salmonicida subsp. salmonicida That Bears a catB3 Gene for Chloramphenicol Resistance

Abstract: Plasmids that carry antibiotic resistance genes occur frequently in Aeromonas salmonicida subsp. salmonicida, an aquatic pathogen with severe consequences in salmonid farming. Here, we describe a 67 kb plasmid found in the A. salmonicida subsp. salmonicida Strain SHY15-2939 from Quebec, Canada. This new plasmid, named pAsa-2939 and identified by high throughput sequencing, displays features never found before in this bacterial species. It contains a transposon related to the Tn21 family, but with an unusual or… Show more

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Cited by 4 publications
(6 citation statements)
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“…Interestingly, transposon instability has been previously observed for plasmid pAsa-2939, an unrelated plasmid to those described in this study that was also found in A. salmonicida subsp. salmonicida (Marcoux et al, 2023a). In subsequent analyses, it will be interesting to verify whether the same phenomenon occurs with pAsa-2900.…”
Section: Resultsmentioning
confidence: 88%
See 1 more Smart Citation
“…Interestingly, transposon instability has been previously observed for plasmid pAsa-2939, an unrelated plasmid to those described in this study that was also found in A. salmonicida subsp. salmonicida (Marcoux et al, 2023a). In subsequent analyses, it will be interesting to verify whether the same phenomenon occurs with pAsa-2900.…”
Section: Resultsmentioning
confidence: 88%
“…Many plasmids found in various A. salmonicida subspecies that carry ARGs have been shown to be transferrable by conjugation to other bacteria from the same genus or to other genera (Sandaa and Enger, 1996;Vincent et al, 2014;Piotrowska and Popowska, 2015;Tanaka et al, 2016;Massicotte et al, 2019;Vincent et al, 2021;Marcoux et al, 2023a, b). The presence of tra genes suggests the ability of a plasmid to conjugate, but this is not always true; for example, pAsa4c is not conjugable in the same conditions tested for other members of the pAsa4 family, although it does bear several tra genes (Tanaka et al, 2016).…”
Section: Introductionmentioning
confidence: 99%
“…To determine the proportion of each version, we used the coverage of Illumina reads for the M16474-11, SHY18-3658, and SHY20-5455 strains. The sequencing data set from the SHY15-2939 strain was used as a negative control because it does not contain pAsa8 [10]. For the fusion between pAsa5 and pAsa8, we mapped the reads against Junction 2 and Junction 0 to compare against the sequence of both plasmids without the insertion.…”
Section: Resultsmentioning
confidence: 99%
“…Interestingly, this excision of the transposon was observed when the plasmid host was A. salmonicida subsp. salmonicida, but not when it was Aeromonas hydrophila [10]. The TTSS locus on pAsa5 is also known to excise from the plasmid.…”
Section: Introductionmentioning
confidence: 99%
“…Some species host different MGE [ 28 ]. A. salmonicida is the species that shows the highest occurrence of mobile genetic elements like plasmids, transposons and the ARG encoded by them [ 29 , 30 ]. The fact that A. trota is the only species susceptible to antibiotics led us to think that this species could be particularly unable to incorporate exogenous DNA.…”
Section: Introductionmentioning
confidence: 99%