Host-Environment Interplay Shapes Fungal Diversity in Mosquitoes

Tawidian, Patil; Coon, Kerri L.; Jumpponen, Ari; Cohnstaedt, Lee W.; Michel, Kristin

doi:10.1128/msphere.00646-21

Cited by 29 publications

(40 citation statements)

References 87 publications

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“…Within the Basidiomycota, the majority of reads were associated with the order Tremellales that contrasts with previous observations that showed a dominance and prevalence of the order Agaricales in all of the water and Ae. albopictus mosquito samples (Tawidian et al 2021). Interestingly, we showed that P. citrinum was one of the most abundant and prevalent species across larvae.…”

Section: Discussionmentioning

confidence: 77%

“…Within the phylum Ascomycota, OTUs in the fungal orders Pleosporales were shared across all water and larvae samples as previously observed in Ae. albopictus (Tawidian et al 2021). In addition, some specific patterns were observed between larvae and water as the majority of reads was assigned to the orders Capnodiales and Hypocreales for water samples and Trichosphaeriales, Eurotiales and Capnodiales for larvae samples.…”

Section: Discussionmentioning

confidence: 99%

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Larval habitat determines the bacterial and fungal microbiota of the mosquito vector Aedes aegypti

Zouache

Martin

Rahola

et al. 2022

FEMS Microbiology Ecology

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Mosquito larvae are naturally exposed to microbial communities present in a variety of larval development sites. Several earlier studies have highlighted that the larval habitat influences the composition of the larval bacterial microbiota. However, little information is available on their fungal microbiota, i.e. the mycobiota. In this study, we provide the first simultaneous characterization of the bacterial and fungal microbiota in field-collected Aedes aegypti larvae and their respective aquatic habitats. We evaluated whether the microbial communities associated with the breeding site may affect the composition of both the bacterial and fungal communities in Ae. aegypti larvae. Our results show a higher similarity in microbial community structure for both bacteria and fungi between larvae and the water in which larvae develop than between larvae from different breeding sites. This supports the hypothesis that larval habitat is a major factor driving microbial composition in mosquito larvae. Since the microbiota plays an important role in mosquito biology, unravelling the network of interactions that operate between bacteria and fungi is essential to better understand the functioning of the mosquito holobiont.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Larval habitat determines the bacterial and fungal microbiota of the mosquito vector Aedes aegypti

Zouache

Martin

Rahola

et al. 2022

FEMS Microbiology Ecology

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“…This paralleled previous study which reported the composition and diversity of fungal communities associated with A. albopictus larvae and their natural habitats. It can be explained by the ubiquity of Ascomycota in freshwater ecosystems, including larval habitats ( Tawidian et al, 2021 ). Aspergillus clavatus (Ascomycota: Trichocomaceae) was previously found to be an opportunistic pathogen of mosquitoes.…”

Section: Discussionmentioning

confidence: 99%

Metagenome Sequencing Reveals the Microbiome of Aedes albopictus and Its Possible Relationship With Dengue Virus Susceptibility

Zhao

Gao

et al. 2022

Front. Microbiol.

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Dengue fever virus (DENV) is a mosquito-borne flavivirus that poses a serious risk to human health. Aedes albopictus is a widely distributed vector of dengue fever in China. Based on the impact of physiological activity, the microbiome in A. albopictus will provide a novel environment-friendly approach to control DENV transmission. We performed metagenomic sequencing on A. albopictus before and after exposure to DENV blood meal to detect microbiome variation of A. albopictus with different susceptibilities to DENV. The dominant phyla in A. albopictus microbiome were Proteobacteria and Ascomycota, and the dominant genera were Aspergillus and Metarhizium. Gammaproteobacteria bacterium, Lactobacillus harbinensis, and Neurospora crassa differed significantly after DENV infection. There were 15 different microorganisms found to be involved in mosquito immunity and metabolism, such as Alphaproteobacteria bacterium, Methyloglobulus morosus, and Shigella sonnei, which might have an impact on the DENV susceptibility of A. albopictus. It was hypothesized that the lack of specific bacteria may lead to increased susceptibility of A. albopictus to DENV. Interventions in the microbiome composition or specific bacteria of A. albopictus may affect the susceptibility to DENV and control the mosquito-borne diseases efficiently.

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“…albopictus and Ae. aegypti larvae (Tawidian et al, 2021;Zouache et al, 2022). However, it remains unclear whether similar patterns in fungal community assembly are observed in the larvae of other mosquito species.…”

Section: Introductionmentioning

confidence: 99%

“…Mosquito larval breeding sites harbor rich bacterial and fungal communities that vary across large and small geographic scales (Gimonneau et al, 2014;Coon et al, 2016;Bascuñán et al, 2018;Shelomi, 2019;Tawidian et al, 2021). The microbiota associated with field-collected mosquito larvae is largely influenced by the bacterial community in the aquatic habitat they reside in.…”

Section: Introductionmentioning

confidence: 99%

Patterns of Fungal Community Assembly Across Two Culex Mosquito Species

2022

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In the aquatic environment, mosquito larvae encounter bacteria and fungi that assemble into bacterial and fungal communities. The composition and impact of mosquito-associated bacterial community has been reported across larvae of various mosquito species. However, knowledge on the composition of mosquito-associated fungal communities and the drivers of their assembly remain largely unclear, particularly across mosquito species. In this study, we used high throughput sequencing of the fungal Internal transcribed spacer 2 (ITS2) metabarcode marker to identify fungal operational taxonomic units (OTUs) and amplicon sequence variants (ASVs) associated with field-collected Culex restuans and Culex pipiens larvae and their breeding water. Our analyses identified diverse fungal communities across larval breeding sites collected on a fine geographic scale. Our data show that the larval breeding site is the major determinant of fungal community assembly in these mosquito species. We also identified distinct fungal communities in guts and carcasses within each species. However, these tissue-specific patterns were less evident in Cx. restuans than in Cx. pipiens larvae. The broad ecological patterns of fungal community assembly in mosquito larvae did not vary between OTU and ASV analyses. Together, this study provides the first insight into the fungal community composition and diversity in field collected Cx. restuans and Cx. pipiens larvae using OTUs and ASVs. While these findings largely recapitulate our previous analyses in Aedes albopictus larvae, we report minor differences in tissue-specific fungal community assembly in Cx. restuans larvae. Our results suggest that while the fungal community assembly in mosquito larvae may be generalized across mosquito species, variation in larval feeding behavior may impact fungal community assembly in the guts of mosquito larvae.

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Host-Environment Interplay Shapes Fungal Diversity in Mosquitoes

Cited by 29 publications

References 87 publications

Larval habitat determines the bacterial and fungal microbiota of the mosquito vector Aedes aegypti

Larval habitat determines the bacterial and fungal microbiota of the mosquito vector Aedes aegypti

Metagenome Sequencing Reveals the Microbiome of Aedes albopictus and Its Possible Relationship With Dengue Virus Susceptibility

Patterns of Fungal Community Assembly Across Two Culex Mosquito Species

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