2020
DOI: 10.7554/elife.55744
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Host-induced spermidine production in motile Pseudomonas aeruginosa triggers phagocytic uptake

Abstract: Exploring the complexity of host-pathogen communication is vital to understand why<br /> microbes persist within a host, while others are cleared. Here, we employed a Dual-sequencing approach to unravel conversational turn-taking of dynamic host-pathogen communications. We demonstrate that upon hitting a host cell, motile Pseudomonas aeruginosa induce a specific gene expression program. This results in the expression of spermidine on the surface, which specifically activates the PIP3-pathway to induce ph… Show more

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Cited by 6 publications
(5 citation statements)
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References 60 publications
(83 reference statements)
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“…Using a gentamicin-protection assay to record bacteria taken up by macrophages, lysates from infections with Δ ntrB , Δ ntrC or Δ ntrBC contained 9.0- to 13.2-fold more bacteria than lysates from infections with WT at 30 min, indicating substantially greater uptake of these mutants. This coincided with significant upregulation of 38 genes important for macrophage uptake ( Felgner et al., 2020 ) ( Table S3 ). There were no statistically significant differences between mutants and WT at 90 min.…”
Section: Resultsmentioning
confidence: 74%
“…Using a gentamicin-protection assay to record bacteria taken up by macrophages, lysates from infections with Δ ntrB , Δ ntrC or Δ ntrBC contained 9.0- to 13.2-fold more bacteria than lysates from infections with WT at 30 min, indicating substantially greater uptake of these mutants. This coincided with significant upregulation of 38 genes important for macrophage uptake ( Felgner et al., 2020 ) ( Table S3 ). There were no statistically significant differences between mutants and WT at 90 min.…”
Section: Resultsmentioning
confidence: 74%
“…Besides their use in P. putida ‐related research, SEVA‐vectors are also becoming useful tools for P. aeruginosa research (Halfon et al ., 2019; Felgner et al ., 2020). Together with standardised DNA assembly, they could improve the efficiency of biotechnological research in this host.…”
Section: Introductionmentioning
confidence: 99%
“…The TspmSyn that we identified corresponds to the PAO1 strain PA4774 gene, also known as speE2 , which encodes the protein NP_253462 (349 a.a.). This gene has been previously described as encoding a SpdSyn ( 52 , 53 ) and a NspdSyn ( 40 ). P. aeruginosa is unusual in that it encodes a typical γ-proteobacterial class 1a AdoMetDC (SpeD) and SpdSyn (SpeE, NP_250378, 286 aa), which are not physically linked in the PAO1 genome.…”
Section: Discussionmentioning
confidence: 99%
“…Biochemical evidence for SpeE2 being a SpdSyn is based on changes to surface-associated Spd levels when the speE2 gene is mutated ( 53 ). This evidence is rather marginal as the reduction of Spd level is only 2-fold.…”
Section: Discussionmentioning
confidence: 99%