Host Manipulation, Gene Editing, and Non-Traditional Model Organisms: A New Frontier for Behavioral Research?

Abstract

Insects and parasites dominate the biosphere, in terms of known biodiversity and mode of life, respectively. Consequently, insects play a part in many host-parasite systems, either as parasite, host, or both. Moreover, a lot of these systems involve adaptive parasite-induced changes of host phenotype (typically behavior or morphology), which is commonly known as host manipulation. While many host manipulation systems have been described within the last few decades, the proximate mechanisms that underpin host phenotypic change are still largely unknown. Given the intimate co-evolutionary history of host-parasite systems, teasing apart the intricate network of biochemical reactions involved in host manipulation requires the integration of various complementary technologies. In this perspective, we stress the importance of multidisciplinary research on host manipulation, such as high-throughput sequencing methods (genomics and transcriptomics) to search for candidate mechanisms that are activated during a manipulation event. Then, we argue that gene editing technologies, specifically the CRISPR-Cas9 system, are a powerful way to test for the functional roles of candidate mechanisms, in both the parasite and the host. Finally, given the sheer diversity of unique host-parasite systems discovered to date, there is indeed a tremendous potential to create novel non-traditional model systems that could greatly expand our capacity to test the fundamental aspects of behavior and behavioral regulation.

Long live the host! Proteomic analysis reveals possible strategies for parasitic manipulation of its social host

“…Not only can potential targeted tissues or metabolic pathways be diverse but also the mechanisms by which parasites influence them. Parasites can cause changes in the host, for example, by their location alone, by energy drain, by inflammation of neural tissue, by influencing the host's epigenome or by protein–protein interactions (Doherty & Matthews, 2022; Lafferty & Shaw, 2013; Martin et al, 2015). By analysing the parasitic proteins present in the host's haemolymph, we gain insight into the latter mechanism.…”

Long live the host! Proteomic analysis reveals possible strategies for parasitic manipulation of its social host

“…But not only the potentially targeted tissues or pathways can be diverse, also the mechanisms, by which parasites can influence them, are. Parasites can cause changes in the host, for example, by their location alone, by energy drain, by inflammation of neural tissue, by influencing the host’s epigenome, or by protein-protein interactions (Doherty & Matthews, 2022; Lafferty & Shaw, 2013; Martin et al, 2015). By analysing the parasitic proteins that can be found in the host’s haemolymph, we gain insight into the latter mechanism.…”