Host modulation therapy using anti-inflammatory and antioxidant agents in periodontitis: A review to a clinical translation

Sulijaya, Benso; Takahashi, Naoki; Yamazaki, Kazuhisa

doi:10.1016/j.archoralbio.2019.07.002

Cited by 48 publications

(44 citation statements)

References 128 publications

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“…The design of an experimental model, capable of mimicking the events that occur when the cells are under endogenous OS related to the GSH depletion, could be useful to correlate with what occurs in vivo, since OS has a key role on PD 33 . In this study, we implemented in GCs a novel model of OS which has been previously tested on neurons in order to evaluate the possible protective effect of MEL against the endogen OS.…”

Section: Discussionmentioning

confidence: 99%

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Melatonin is an effective protector of gingival cells damaged by the cytotoxic effect of glutamate and DL‐buthionine sulfoximine

Solá

Aguilar

Mosquera

et al. 2020

J of Periodontal Research

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Background and Objective Cellular damage related to oxidative stress (OS) is implicated in periodontal diseases (PD). Melatonin (MEL) has multiple functions, and it has been described as a potential treatment for PD. We aim at evaluating the protective effects of MEL on an in vitro model of cellular damage triggered by glutamate (GLUT) and DL‐buthionine sulfoximine (BSO), on gingival cells (GCs) in culture. Material and Methods A primary culture of GCs from Wistar rats was developed in order to test the protective property of MEL; BSO and GLUT were administered alone as well as in combination with MEL. The viability and apoptosis were measured with MTT assay and TUNEL, respectively, and the concentration of superoxide anion (O2‐) was measured with the NBT method. Results The combination of BSO and GLUT treatment resulted in a decreased viability of GCs. This was evidenced by the increase in both the production of superoxide anion and apoptosis. After MEL administration, the oxidant and pro‐apoptotic effects of BSO and GLUT were totally counteracted. Conclusions These findings demonstrated that MEL has an effective protective role on GCs subjected to cellular damage in a model of OS and cytotoxicity triggered by BSO and GLUT. Consequently, MEL could be used as a therapeutic agent in PD which begin with a significative loss of GCs.

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Section: Discussionmentioning

confidence: 99%

“…vivo, since OS has a key role on PD. 33 In this study, we implemented in GCs a novel model of OS which has been previously tested on neurons in order to evaluate the possible protective effect of MEL against the endogen OS.…”

Section: To Evaluate Whether the Decreased Cell Viability With Bso Andmentioning

confidence: 99%

Melatonin is an effective protector of gingival cells damaged by the cytotoxic effect of glutamate and DL‐buthionine sulfoximine

Solá

Aguilar

Mosquera

et al. 2020

J of Periodontal Research

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“…Inflammatory osteolysis is a critical pathological feature of clinical inflammatory diseases such as periodontitis which is one of the most common oral diseases and associated with alveolar bone resorption and tooth loosening in adults [1]. The therapeutics for periodontitis are intended to block inflammation locally, but not enough for inflammatory bone destruction [2]. However, many patients still suffer from bone loss under the anti-inflammation treatments [3].…”

Section: Introductionmentioning

confidence: 99%

PTEN Inhibits Inflammatory Bone Loss in Ligature-Induced Periodontitis via IL1 and TNF-α

Wei

Zhang

2019

BioMed Research International

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Phosphatase and tensin homolog (PTEN) is a critical regulator of tumorigenesis and bone remodeling, which is also found expressed in the periodontal tissues. Periodontitis is one of the most common oral diseases and associated with alveolar bone resorption and tooth loosening in adults. However, the functional relevance of PTEN in periodontitis remains unclear. Here, we report that PTEN plays an essential role in periodontitis. The in vivo results of our study showed a significant decrease of PTEN in the ligature-induced mouse periodontitis model. The function of PTEN in the macrophages was shown to be associated with inflammatory factors interleukin 1 (IL1) and tumor necrosis factor (TNF-α) by using overexpression and silence methods. Further mechanistic studies indicated lack of PTEN-activated IL1 and TNF-α, which increased the number of osteoclasts and led to alveolar bone erosion and loss. Moreover, PTEN nanoparticles could directly inhibit the inflammatory process and bone erosion, suggesting a controlling role of PTEN during bone remodeling. All these data identified the novel function of PTEN as a key factor in periodontitis and bone remodeling.

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“…Periodontitis is essentially the consequence of the dysregulated host-microbial interactions. Recently, therapeutic management towards the host immunoinflammatory response has been proposed as a promising approach [23]. Indeed, several compounds from Chinese medicinal herbs have been suggested for the treatment of periodontal disease, e.g., osthole from Cnidium monnieri [24], shikonin from Radix Arnebiae [25], psoralen and angelicin from Psoraleae [26].…”

Section: Discussionmentioning

confidence: 99%

Baicalein inhibits inflammatory response and promotes osteogenic activity in periodontal ligament cells challenged with lipopolysaccharides

Ren

Zhao

et al. 2021

BMC Complement Med Ther

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Background Periodontitis is a chronic infection initiated by oral bacterial and their virulence factors, yet the severity of periodontitis is largely determined by the dysregulated host immuno-inflammatory response. Baicalein is a flavonoid extracted from Scutellaria baicalensis with promising anti-inflammatory properties. This study aims to clarify the anti-inflammatory and osteogenic effects of baicalein in periodontal ligament cells (PDLCs) treated with lipopolysaccharides (LPS). Methods Human PDLCs were incubated with baicalein (0–100 μM) for 2 h prior to LPS challenge for 24 h. MTT analysis was adopted to assess the cytoxicity of baicalein. The mRNA and protein expression of inflammatory and osteogenic markers were measured by real-time polymerase chain reaction (PCR), western blot and enzyme-linked immunosorbent assay (ELISA) as appropriate. Alkaline phosphatase (ALP) and Alizarin red S (ARS) staining were performed to evaluate the osteogenic differentiation of PDLCs. The expression of Wnt/β-catenin and mitogen-activated protein kinase (MAPK) signaling related proteins was assessed by western blot. Results MTT results showed that baicalein up to 100 μM had no cytotoxicity on PDLCs. Baicalein significantly attenuated the inflammatory factors induced by LPS, including interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), matrix metalloprotein-1 (MMP-1), MMP-2 and monocyte chemoattractant protein 1 (MCP-1) at both mRNA and protein level. Moreover, MAPK signaling (ERK, JNK and p38) was significantly inhibited by baicalein, which may account for the mitigated inflammatory response. Next, we found that baicalein effectively restored the osteogenic differentiation of LPS-treated PDLCs, as shown by the increased ALP and ARS staining. Accordingly, the protein and gene expression of osteogenic markers, namely runt-related transcription factor 2 (RUNX2), collagen-I, and osterix were markedly upregulated. Importantly, baicalein could function as the Wnt/β-catenin signaling activator, which may lead to the increased osteoblastic differentiation of PDLCs. Conclusions With the limitation of the study, we provide in vitro evidence that baicalein ameliorates inflammatory response and restores osteogenesis in PDLCs challenged with LPS, indicating its potential use as the host response modulator for the management of periodontitis.

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Host modulation therapy using anti-inflammatory and antioxidant agents in periodontitis: A review to a clinical translation

Cited by 48 publications

References 128 publications

Melatonin is an effective protector of gingival cells damaged by the cytotoxic effect of glutamate and DL‐buthionine sulfoximine

Melatonin is an effective protector of gingival cells damaged by the cytotoxic effect of glutamate and DL‐buthionine sulfoximine

PTEN Inhibits Inflammatory Bone Loss in Ligature-Induced Periodontitis via IL1 and TNF-α

Baicalein inhibits inflammatory response and promotes osteogenic activity in periodontal ligament cells challenged with lipopolysaccharides

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