Host-Range Phylogenetic Grouping of Capripoxviruses

Goff, Christian Le; Fakhfakh, Emna; Chadeyras, Amélie; Adulugba, Elexpeter Aba; Libeau, Geneviève; Hammami, Salah; Diallo, Adama; Albina, Emmanuel

doi:10.1007/1-4020-3312-5_58

Cited by 13 publications

(8 citation statements)

References 14 publications

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“…Recently, a capripoxvirus real‐time PCR method using primers and a probe previously described (Bowden et al., 2008) has been validated (Stubbs et al., submitted). Sequence analysis of the P32 gene has revealed differences between sheep pox and goat pox viruses (Hosamani et al., 2004), and further sequence analysis of a putative gene encoding a homologue G‐protein‐coupled chemokine receptor (Q2/3L) (Cao et al., 1995) from 26 capripoxvirus isolates revealed three distinct clusters that consisted of sheep pox virus, goat pox virus and LSDV (Le Goff et al., 2005). To carry out a wider phylogenetic analysis, the same gene from another 58 capripoxvirus isolates was sequenced (Le Goff et al., 2009).…”

Section: Novel Diagnostic Testsmentioning

confidence: 99%

Review: Lumpy Skin Disease: An Emerging Threat to Europe, the Middle East and Asia

Tuppurainen

Oura

2011

Transboundary and Emerging Diseases

354

416

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Lumpy skin disease (LSD) is an economically devastating emerging viral disease of cattle. Lumpy skin disease is currently endemic in most African countries and has recently spread out of Africa into the Middle East region. In this article, we review the putative mechanisms of spread of LSD into the Middle East and the risks of further spread into Turkey, Europe and Asia. We also review the latest findings on the epidemiology of LSD, its mechanisms of transmission, the potential role of wildlife in its maintenance and spread and the diagnostic tests and control methods currently available.

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Section: Novel Diagnostic Testsmentioning

confidence: 99%

Review: Lumpy Skin Disease: An Emerging Threat to Europe, the Middle East and Asia

Tuppurainen

Oura

2011

Transboundary and Emerging Diseases

354

416

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“…Two conventional agarose gel‐based polymerase chain reaction (PCR) assays were optimized using skin lesions, nodules and crusts. The target genes were the thymidine kinase gene (Gershon & Black, ) and a putative gene encoding the homolog G‐protein‐coupled chemokine receptor (Q2/3L) named the GPCR and the homolog interleukin gene receptor (IL8) (Cao, Gershon, & Black, ; Le Goff et al., , ). However, the results obtained could not differentiate between SPPV, GTPV and LSDV (Ireland & Binepal, ; Kitching & Smale, ) and this was considered as a major constraint to the interpretation of the data.…”

Section: Sheep Pox In Tunisiamentioning

confidence: 99%

Sheep pox in Tunisia: Current status and perspectives

Chehida

Ayari-Fakhfakh

Caufour

et al. 2017

Transbound Emerg Dis

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Sheep pox, a well-known endemic capripox infection, has significant impacts on small ruminant populations in Tunisia. It is responsible for high economic losses throughout North Africa due to its enzootic nature and to the active animal transhumance existing in some governorates in Tunisia. The aim of this review was to analyse data gathered on annual vaccination campaigns designed to control its spread by reducing the level of endemicity and to describe diagnostic and management tools adapted to the Tunisian situation. Seasonal, temporal and spatial distributions of sheep pox outbreaks, as well as related clinical features, were found. It was concluded from this review that establishing strong herd immunization through individual animal immunization, creating adequate infrastructure, increasing awareness among breeders, setting up a field-based surveillance network and improving routine diagnostic methods need to be the major components of a programme to eradicate the disease. It was also felt that cost-benefit analyses of the surveillance and control strategies used would help in controlling its persistence.

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“…The Gprotein-coupled chemokine receptor (GPCR) gene is one of the variable genes within Capripoxviruses [23] and is an appropriate target for genetic distinction between Capripoxviruses [24]. The suitability of the GPCR gene for host range phylogenetic grouping was described by Le Goff et al 2005 [25] and has been used by various authors to characterise Capripoxviruses [21,22,[26][27][28][29]. The GPCR gene encodes a protein related to the G-protein-coupled chemokine receptor subfamily.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and phylogenetic analysis of lumpy skin disease virus from outbreaks in Uganda 2017–2018

et al. 2020

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Background: Lumpy skin disease (LSD) is an infectious viral disease of cattle caused by a Capripoxvirus. LSD has substantial economic implications, with infection resulting in permanent damage to the skin of affected animals which lowers their commercial value. In Uganda, LSD is endemic and cases of the disease are frequently reported to government authorities. This study was undertaken to molecularly characterize lumpy skin disease virus (LSDV) strains that have been circulating in Uganda between 2017 and 2018. Secondly, the study aimed to determine the phylogenetic relatedness of Ugandan LSDV sequences with published sequences, available in GenBank. Results: A total of 7 blood samples and 16 skin nodule biopsies were screened for LSDV using PCR to confirm presence of LSDV nucleic acids. PCR positive samples were then characterised by amplifying the GPCR gene. These amplified genes were sequenced and phylogenetic trees were constructed. Out of the 23 samples analysed, 15 were positive for LSDV by PCR (65.2%). The LSDV GPCR sequences analysed contained the unique signatures of LSDV (A11, T12, T34, S99, and P199) which further confirmed their identity. Sequence comparison with vaccine strains revealed a 12 bp deletion unique to Ugandan outbreak strains. Phylogenetic analysis indicated that the LSDV sequences from this study clustered closely with sequences from neighboring East African countries and with LSDV strains from recent outbreaks in Europe. It was noted that the sequence diversity amongst LSDV strains from Africa was higher than diversity from Eurasia. Conclusion: The LSDV strains circulating in Uganda were closely related with sequences from neighboring African countries and from Eurasia. Comparison of the GPCR gene showed that outbreak strains differed from vaccine strains. This information is necessary to understand LSDV molecular epidemiology and to contribute knowledge towards the development of control strategies by the Government of Uganda.

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Host-Range Phylogenetic Grouping of Capripoxviruses

Cited by 13 publications

References 14 publications

Review: Lumpy Skin Disease: An Emerging Threat to Europe, the Middle East and Asia

Review: Lumpy Skin Disease: An Emerging Threat to Europe, the Middle East and Asia

Sheep pox in Tunisia: Current status and perspectives

Molecular detection and phylogenetic analysis of lumpy skin disease virus from outbreaks in Uganda 2017–2018

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