Host range, symptom expression and RNA 3 sequence analyses of six Australian strains of<i>Cucumber mosaic virus</i>

Sulistyowati, Emy; Mitter, Neena; Bastiaan‐Net, Shanna; Roossinck, Marilyn J.; Dietzgen, Ralf G.

doi:10.1071/ap04054

Cited by 22 publications

(13 citation statements)

References 22 publications

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“…In newly developing tomato leaves, viral isolate induced symptoms ranging from severe chlorotic patches, downward curling and reduced leaf lamina to mild filiformism. Previously, shoestring like leaves symptoms were reported only with some subgroup I strains [2,20,32,46,48]. From India also there was report of mosaic symptoms on tomato by subgroup II strain [47] and now in a recent observation it was found that shoestring symptoms were not restricted to subgroup I.…”

Section: Discussionmentioning

confidence: 90%

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

et al. 2013

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Cucumber mosaic virus (CMV) has a wide host range causing severe damage in many important agricultural and ornamental crops. Earlier reports showed the prevalence of CMV subgroup I isolates in India. However, some recent reports point towards increasing incidence of subgroup II isolates in the country. The complete genome of a CMV isolate causing severe mosaic in cucumber was characterized and its phylogenetic analysis with other 21 CMV isolates reported worldwide clustered it with subgroup II strains. The genome comprised of RNA 1 (3,379 nucleotides), RNA 2 (3,038 nucleotides) and RNA 3 (2,206 nucleotides). The isolate showed highest homology with subgroup II isolates: 95.1-98.7, 87.7-98.0, and 85.4-97.1 % within RNA1, RNA2, and RNA3, respectively. RNA1 and RNA2 were closely related to the Japanese isolate while RNA3 clustered with an American isolate. Host range studies revealed that isolate showed severe mosaic symptoms on Nicotiana spp. and Cucumis spp. The isolate induced leaf deformation and mild filiform type symptoms in tomato. To best of our knowledge this is the first report of complete genome of CMV subgroup II isolate from India.

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Section: Discussionmentioning

confidence: 90%

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

et al. 2013

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“…Shoe-string symptoms caused by CMV has been reported from India, which was grouped under subgroup-IB [14], whereas CMV isolated from tomato showing shoe-string in Pakistan [2] and Australia [30] were grouped under subgroup-IA and -IB, respectively. In the present study, CMV isolate showing shoe-string in tomato (Tss-In) is grouped under subgroup-II.…”

Section: Discussionmentioning

confidence: 97%

Biological and Molecular Characterization of Two Distinct Tomato Strains of Cucumber mosaic virus Based on Complete RNA-3 Genome and Subgroup Specific Diagnosis

et al. 2011

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Cucumber mosaic virus (CMV) is one of the most important viral pathogen infecting several plant species in India. Five isolates of CMV obtained from cucumber, muskmelon, tobacco and tomato from distinct geographical locations in India were analysed based on host-reactions and genome sequence. The majority of the isolates were very similar and only two isolates, Tfr-In and Tss-In showed distinct symptoms in tomato and high sequence diversity (77.8%) in coat protein (CP) gene. Tfr-In was isolated from tomato fruit showing grey patches in Aurangabad and Tss-In from tomato plant showing shoe-string symptoms in New Delhi. The RNA-3 genomes of Tfr-In (2,214 nt; JF279606), shared only 70.3% nucleotide sequence identity with Tss-In (2,178 nt; JF279605. The complete RNA-3 genome of Tss-In and Tfr-In were compared with that of 65 CMV isolates reported from various plants of the world, which formed four distinct subclades-IA, -IB, -IC and -II. The Tfr-In isolate clustered with the CMV subgroup-IB and Tss-In with the subgroup-II. The comparison of the RNA-3 sequence of both the isolates revealed maximum heterogeneity in the intergenic region (IR). Reverse transcriptase polymerase chain reaction (RT-PCR) based detection of CMV subgroup-I and -II was developed designing primers from flanking IR region. The specificity of the RT-PCR detection was confirmed using Tfr-In and Tss-In representing subgroup-I and -II and validated with field samples of tomato, cucurbits and chilli. This is the first report of complete RNA-3 of subgroup-IB CMV causing grey patches in tomato fruit and subgroup-II CMV causing shoe-string symptoms in tomato in India. The present and previous studies together showed that tomato in India was affected by multiple strains of CMV.

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“…Proteins 1a, 2a and 3a are expressed from genomic RNAs 1, 2 and 3 respectively, whereas the coat protein (CP) and 2b proteins are expressed from subgenomic RNA4 and RNA 4A, respectively (Palukaitis et al 1992;Ding et al 1994;Sulistyowati et al 2004). The 1a and 2a proteins are apart of viral replicase complex (Hayes and Buck 1990), while cell to cell movement through the plant requires the 3a movement protein (MP) and coat protein (CP) (Suzuki et al 1991;Taliansky and Garcia-Arenal 1995;Kaplan et al 1995;Gal-On et al 1998).…”

Section: Introductionmentioning

confidence: 97%

“…Tomato is found to be infected with all three subgroups of CMV (Sudhakar et al 2006;Akhtar et al 2008;Pratap et al 2008). CMV infected plants show a broad spectrum of symptoms from mild to severe mosaic, stunting, filiformity, chlorosis and necrosis depending on viral strain and host (Carrere et al 1999;Sulistyowati et al 2004;García-Arenal and Palukaitis 2008). In tomato, some subgroup I strains can cause fern-shaped filiform leaves and stunting (Hellwald et al 2000;Stamova and Chetelat 2000;Akhtar et al 2008;Pratap et al 2008) whereas subgroup II strains lead to severe mosaic, leaf puckering and stunting (Sudhakar et al 2006).…”

Section: Introductionmentioning

confidence: 97%

Resistance of Solanum species to Cucumber mosaic virus subgroup IA and its vector Myzus persicae

et al. 2010

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Sixty-nine tomato genotypes representing nine Solanum species were evaluated for resistance to Cucumber mosaic virus (CMV) subgroup IA and its aphid vector Myzus persicae. Resistance was assessed by visual scoring of symptoms in the field under natural conditions, and in the greenhouse by artificial inoculations through aphid M. persicae and mechanical transmissions in the year 2007 and 2009. Considerable variation in responses was observed among the evaluation methods used. Field evaluations were found liable to errors as different levels were observed for the same genotypes in the different years, however mechanical inoculation was found to be the most useful in identifying CMV subgroup IA resistance, in contrast aphid transmission was most useful in identifying insect transmission resistance. All genotypes observed as highly resistant to CMV subgroup IA in the field or through vector transmission became systemically infected through mechanical inoculations. Using mechanical inoculation, six genotypes (TMS-1 of S. lycopersicum, LA1963 and L06049 of S. chilense, LA1353, L06145 and L06223 of S. habrochaites)were found resistant and another six (L06188 and L06238 of S. neorickii, L06219 of S. habrochaites, L05763, L05776 and L06240 of S. pennellii) were found tolerant showing mild symptoms with severity index (SI) ranging 1-2 and with delayed disease development after a latent period (LP) of 18-30 days. However, these genotypes were found to be resistant to highly resistant in the field and through inoculation by M. persicae; and they also supported low population levels of M. persicae except TMS-1. Another nine genotypes (LA2184 of S. pimpinellifolium L., LA2727 of S. neorickii, LA0111, L06221, L06127 and L06231 of S. peruvianum L., LA1306, L06057 and L06208 of S. chmielewskii) showing a susceptible response after mechanical inoculation were highly resistant, resistant and tolerant after M. persicae transmission. The resistant genotypes, identified in the present study can be exploited in the breeding programmes aimed at developing tomato varieties resistant to CMV subgroup IA and broadening the genetic base of CMV-resistant germplasm. The differences observed between mechanical and aphid transmission suggests that one should consider both evaluation methods for tomato germplasm screening against CMV subgroup IA.

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Host range, symptom expression and RNA 3 sequence analyses of six Australian strains ofCucumber mosaic virus

Cited by 22 publications

References 22 publications

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

Biological and Molecular Characterization of Two Distinct Tomato Strains of Cucumber mosaic virus Based on Complete RNA-3 Genome and Subgroup Specific Diagnosis

Resistance of Solanum species to Cucumber mosaic virus subgroup IA and its vector Myzus persicae

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