Host response to black sigatoka in Musa germplasm of different ages under natural inoculation conditions

Mobambo, K.N.; Pasberg‐Gauhl, C.; Gauhl, F.; Zuofa, K.

doi:10.1016/s0261-2194(96)00112-3

Cited by 3 publications

(1 citation statement)

References 2 publications

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“…Calcutta 4, but these did not increase in size until the end of the experiment. This is consistent with field studies of resistance of different banana cultivars, which have shown that Calcutta 4 is highly resistant and develops defence-related necrotic lesions in the field that do not increase in size (Mobambo, et al 1997;Orjeda et al 2000). When considering the level of resistance of the two banana cultivars and the defence responses activated, it is important to bear in mind that they have different ploidy levels, Williams being triploid (AAA) whereas Calcutta 4 is diploid (AA).…”

Section: Plant Responses To Mycosphaerella Fijiensissupporting

confidence: 86%

Differential induction of pathogenesis-related proteins in banana in response to Mycosphaerella fijiensis infection

et al. 2012

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Black leaf streak or "black Sigatoka" is one of the most important diseases affecting bananas and plantains worldwide. Very few studies have been published on the host-pathogen interaction of this pathosystem, particularly at the molecular level. The aim of this work was to analyze, under controlled conditions, the enzyme activity of peroxidase (POX), phenylalanine ammonia lyase (PAL), β-1, 3-glucanase (GLU) and chitinase (CHI) as well as the production of H 2 O 2 in banana plants infected with Mycosphaerella fijiensis. Defence responses were examined and compared in a resistant (Calcutta 4) and a susceptible (Williams) cultivar. Plants were inoculated and tested for enzyme activity at 0, 6, 12, 18, 24, 48 and 72 h after infection (HAI) and 6, 9, 12, 15 and 18 days after inoculation (DAI). A rapid induction of PAL, POX and GLU was observed in the resistant cultivar at 6-18 HAI as well as H 2 O 2 production at 72 HAI. In contrast, in the susceptible cultivar, induction of these enzymes was only observed from 6 DAI. These results suggest that the first 72 HAI are important in determining the response of the host to the disease. Further studies characterizing banana responses to M. fijiensis at the early stages of the infection are necessary in order to better understand this host-pathogen interaction.

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Section: Plant Responses To Mycosphaerella Fijiensissupporting

confidence: 86%

Differential induction of pathogenesis-related proteins in banana in response to Mycosphaerella fijiensis infection

et al. 2012

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Rapid Screening of Musa Species for Resistance to Black Leaf Streak Using In Vitro Plantlets in Tubes and Detached Leaves

et al. 2007

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This study investigated the utility of inoculation of in vitro plantlets in tubes and detached leaves as reliable and rapid assays for screening Musa genotypes against Mycosphaerella fijiensis, the causal agent of black leaf streak. In the first part of the study, three types of inocula were evaluated to determine suitability for in vitro inoculation. Inoculation of in vitro plantlets with mycelial fragments resulted in significantly (P < 0.05) higher levels of disease severity and faster rates of disease progress compared with inoculations using conidial suspensions. In the detached leaf assay, amending agar medium with plant hormones significantly (P < 0.0001) aided retention of green leaf color. Leaf pieces on medium containing gibberellic acid at 5 mg/liter had about 5% chlorosis at 52 days after plating. When in vitro plantlets in tubes and detached leaves of 10 Musa genotypes with different levels of disease resistance were inoculated with M. fijiensis, there were significant (P < 0.05) differences among genotypes in leaf area infected, incubation time, and symptom evolution time. For incubation time and leaf area infected, cultivars responded depending on their level of disease resistance, with resistant genotypes Calcutta-4 and PITA-17 having significantly (P = 0.001) longer incubation times and lower infected leaf areas compared with the susceptible cultivar Agbagba and moderately resistant cultivar FHIA-23. A similar pattern in cultivar response was observed for symptom evolution time. Leaf area infected was not significantly (P = 0.2817 for two-tailed t test) different when assessed using the two assays, and infected leaf areas in both assays were strongly correlated (r = 0.88, n = 48, P < 0.0001). Although incubation times were significantly (P = 0.0062 for two-tailed t test) different between the two assays, values from the two assays were strongly correlated (r = 0.69, n = 48, P < 0.0001). These results show that these two assays are rapid and space-effective, and can reliably be used for screening Musa genotypes for resistance to black leaf streak.

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A Quantitative Assay Using Mycelial Fragments to Assess Virulence of Mycosphaerella fijiensis

Donzelli¹,

Churchill²

2007

Phytopathology®

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We describe a method to evaluate the virulence of Mycosphaerella fijiensis, the causal agent of black leaf streak disease (BLSD) of banana and plantain. The method is based on the delivery of weighed slurries of fragmented mycelia by camel's hair brush to 5-by-5-cm areas on the abaxial surface of banana leaf blades. Reliable BLSD development was attained in an environmental growth chamber with stringent lighting and humidity controls. By localizing inoculum onto small areas of large leaves, we achieved a dramatic increase in the number of strains that can be tested on each leaf and plant, which is critical for comparing the virulence of numerous strains concurrently. Image analysis software was used to measure the percentage of each inoculated leaf section showing BLSD symptoms over time. We demonstrated that the level of disease of four isolates was correlated with the weight of the mycelium applied and relatively insensitive to the degree of fragmentation of hyphae. This is the first report demonstrating that weighed mycelial inoculum, combined with image analysis software to measure disease severity, can be used to quantitatively assess the virulence of M. fijiensis under rigorously controlled environmental conditions.

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Host response to black sigatoka in Musa germplasm of different ages under natural inoculation conditions

Cited by 3 publications

References 2 publications

Differential induction of pathogenesis-related proteins in banana in response to Mycosphaerella fijiensis infection

Differential induction of pathogenesis-related proteins in banana in response to Mycosphaerella fijiensis infection

Rapid Screening of Musa Species for Resistance to Black Leaf Streak Using In Vitro Plantlets in Tubes and Detached Leaves

A Quantitative Assay Using Mycelial Fragments to Assess Virulence of Mycosphaerella fijiensis

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