Host Response to Eggs of Schistosoma Mansoni: III. The Role of Eggs in Resistance

Vonlichtenberg, F; Eh, Sadun; Ji, Bruce

doi:10.1093/infdis/113.2.113

Cited by 21 publications

(5 citation statements)

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“…Our data, taken together with the results of these earlier studies, suggest the possibility that the inability to demonstrate protection in mice immunized with eggs or complex egg extracts results from suppression of the cell-mediated immune response, rather than the lack of protective antibody. We have demonstrated here, as have Lichtenberg et al (8) and Bickle et al (10) in earlier studies, that immunization with eggs gives rise to antibodies that recognize surface membrane epitopes on cercariae and schistosomula. Thus, if there is a suppressive response to avoid, it may be necessary to immunize with only one or a limited number of egg epitopes.…”

Section: Discussionsupporting

confidence: 88%

Schistosoma mansoni. Anti-egg monoclonal antibodies protect against cercarial challenge in vivo.

Harn¹,

Mitsuyama²,

David³

1984

The Journal of Experimental Medicine

125

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Monoclonal antibodies that bind to surface membranes of developing schistosomula and/or cercarial tails were generated from mice immunized with living schistosome eggs or soluble egg antigen. These monoclonal antibodies detected at least three different surface epitopes. One surface antigen detected by anti-egg monoclonal antibody EG1C4B1 (E.1) persisted on the surface of developing schistosomula for 96 h posttransformation . The same or a cross-reactive antigen was also detected on the surfaces of S. japonicum and S. haematobium schistosomula and cercarial tails. Monoclonal antibody E.1 killed schistosomula in vitro as well or better than infected mouse sera and transferred immunity to naive mice when administered in vivo. The monoclonal antibody reduced the number of lung worms recoverable on day 4 postchallenge by up to 85% and reduced the adult worm burden up to 41% as compared with controls. The data also show that the molecular weights of the egg antigens detected by monoclonal antibody E.1 were different from those detected on schistosomula.

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Section: Discussionsupporting

confidence: 88%

Schistosoma mansoni. Anti-egg monoclonal antibodies protect against cercarial challenge in vivo.

Harn¹,

Mitsuyama²,

David³

1984

The Journal of Experimental Medicine

125

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“…We have failed to reproduce this result (Long et al 1980) and, in further experiments (Table 6), no resistance was induced in CBA mice by injection of intact eggs intraperitoneally, intravenously or subcutaneously. Other attempts to induce resistance by systemic injection of intact S. mansoni eggs or egg constituents have also failed (Moore, Crandall & Hunter, 1963;von Lichtenberg, Sadun & Bruce, 1963;Smithers, 1962;Bickle etal. 1979;Doenhoff, Bickle, Bain, Webbe & Nelson, 1980).…”

Section: Discussionmentioning

confidence: 99%

Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. Evidence that the mechanisms which mediate resistance during early patent infections may lack immunological specificity

1982

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SUMMARYMice infected with 25 to 35 unsexed Schistosoma mansoni cercariae became resistant to homologous challenge by 8 weeks, and the degree of resistance that was acquired correlated with the size of the worm burden and the tissue egg burden. Mice given 100 male or 100 female cercariae alone failed to become resistant even after 30–40 weeks of infection. When a super-infection of 100 male or 100–300 female cercariae was given to mice with 25 unsexed cercariae, the degree of resistance that was acquired was generally lower than in mice given 25 unsexed cercariae alone. In the super-infected mice there was no correlation between the size of the worm burden (mainly unpaired male or female worms) and the degree of acquired resistance. In mice super-infected with male worms there was an inverse correlation between the number of male worms and the number of eggs deposited in the liver and intestine by each mature worm pair. A positive correlation was, however, still found between the degree of acquired resistance and total tissue egg counts in mice super-infected with single sex cercariae. Mice injected intraperitoneally or subcutaneously with intact S. mansoni eggs failed to become resistant to S. mansoni challenge. Transfer of lymphoid cells or serum, separately or together, from heavily infected and demonstrably resistant donor mice to naive normal or T-cell deprived recipients failed to render the recipients resistant to S. mansoni challenge. The results indicate that resistance to re-infection in mice with recently patent S. mansoni infections is dependent on the number of S. mansoni eggs entrapped in the tissues, and that excessive numbers of unpaired S. mansoni male or female worms can inhibit the fecundity of established worm pairs. The apparent acquired resistance to S. mansoni that has been observed in this model system may be largely a consequence of non-specific factors interfering with normal patterns of challenge worm migration and maturation.

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Section: /7 Micementioning

confidence: 98%

“…These diffuse foci (approximate diameter 294 ± SE 5 m m) rarely contained trapped larvae and comprised predominantly epithelioid macrophages and PMN, with multinucleated giant cells of various sizes dispersed throughout. 22 The generalized infiltration through all lung tissues consisted primarily of enlarged alveolar macrophages (diameter 12 . 7 ± SE 1 .…”

Section: /7 Micementioning

confidence: 99%

“…3f ) reminiscent of the pathology generated by embolization of schistosome eggs in the lung. 22 It must be emphasized that age-matched, naive, sentinel IFN-gR 7 /7 mice lacked any pulmonary infiltrates. BAL cultures from the three groups of mice produced distinctive patterns of cytokines when stimulated in vitro with larval antigen (Fig.…”

Section: Airway Pmn and Lymphocyte Numbers Are Elevated And Macrophamentioning

confidence: 99%

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Impaired immunity and altered pulmonary responses in mice with a disrupted interferon‐γ receptor gene exposed to the irradiated Schistosoma mansoni vaccine

et al. 1996

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SUMMARYA high level of protection against Schistosoma mansoni is elicited in mice by the irradiated cercaria vaccine and interferon-g (IFN-g) is a key cytokine in the pulmonary effector response. The role of this cytokine has been investigated in mice with a targeted disruption of the IFN-g receptor gene (IFN-gR ÿ /ÿ mice). The level of protection was impaired relative to that elicited in C57BL/6 and 129 wild-type (WT) animals. These two groups developed compact effector foci, of largely mononuclear cell composition, around individual challenge parasites migrating through the lungs. In contrast the IFN-gR ÿ /ÿ mice showed a massive and generalized leucocytic infiltration of the airways and interstitium in which eosinophils were a prominent feature. Cultures of airway leucocytes from C57BL/6 mice produced abundant IFN-g whilst those from IFN-gR ÿ /ÿ mice produced interleukin-4 (IL-4), IL-5 and IL-10, indicating default to the Th2 pathway; the WT animals showed an intermediate response. The pattern of cytokine gene transcripts in whole lung tissue agreed remarkably well with the level of cytokine protein detected in leucocyte cultures, with the exception of substantial IL-4 mRNA but negligible protein in C57BL/6 mice. The loose but intense infiltrate of leucocytes in the lungs of IFN-gR ÿ /ÿ mice was clearly ineffective in eliminating challenge parasites, whereas the level of IFN-g protein and mRNA in the lungs of C57BL/6 and WT mice correlated with the size and compactness of effector foci. On the basis of these and earlier observations, we suggest that a primary role for IFN-g is to promote intercelluar adhesion between the leucocytes in an effector focus, promoting its ability to block parasite migration.

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Host Response to Eggs of Schistosoma Mansoni: III. The Role of Eggs in Resistance

Cited by 21 publications

References 0 publications

Schistosoma mansoni. Anti-egg monoclonal antibodies protect against cercarial challenge in vivo.

Schistosoma mansoni. Anti-egg monoclonal antibodies protect against cercarial challenge in vivo.

Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. Evidence that the mechanisms which mediate resistance during early patent infections may lack immunological specificity

Impaired immunity and altered pulmonary responses in mice with a disrupted interferon‐γ receptor gene exposed to the irradiated Schistosoma mansoni vaccine

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