Host to a Stranger: Arabidopsis and Fusarium Ear Blight

Brewer, Helen C.; Hammond‐Kosack, K. E.

doi:10.1016/j.tplants.2015.06.011

Cited by 21 publications

(18 citation statements)

References 87 publications

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Section: Conclusion and Future Prospectsmentioning

confidence: 99%

“…The large and complex genomes of wheat and other cereals seem to be another factor that has impeded genomic studies (Brewer and Hammond-Kosack, 2015). For this reason, in the past, the interaction between F. graminearum and the model dicot host Arabidopsis has been used to understand host resistance to F. graminearum (Brewer and Hammond-Kosack, 2015). The genetic and genomic resources available in the model monocot plant Brachypodium distachyon, which is known to be susceptible to many cereal pathogens, including F. graminearum (Fitzgerald et al, 2015a;Pasquet et al, 2014Pasquet et al, , 2016Peraldi et al, 2011), can also be exploited to overcome potential difficulties encountered as a result of the genetic complexity of cereal genomes, and to identify host genes regulating disease/toxin resistance or susceptibility.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

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Transcriptomics of cereal–Fusarium graminearum interactions: what we have learned so far

Kazan

Gardiner

2017

Molecular Plant Pathology

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The ascomycete fungal pathogen Fusarium graminearum causes the globally important Fusarium head blight (FHB) disease on cereal hosts, such as wheat and barley. In addition to reducing grain yield, infection by this pathogen causes major quality losses. In particular, the contamination of food and feed with the F. graminearum trichothecene toxin deoxynivalenol (DON) can have many adverse short- and long-term effects on human and animal health. During the last decade, the interaction between F. graminearum and both cereal and model hosts has been extensively studied through transcriptomic analyses. In this review, we present an overview of how such analyses have advanced our understanding of this economically important plant-microbe interaction. From a host point of view, the transcriptomes of FHB-resistant and FHB-susceptible cereal genotypes, including near-isogenic lines (NILs) that differ by the presence or absence of quantitative trait loci (QTLs), have been studied to understand the mechanisms of disease resistance afforded by such QTLs. Transcriptomic analyses employed to dissect host responses to DON have facilitated the identification of the genes involved in toxin detoxification and disease resistance. From the pathogen point of view, the transcriptome of F. graminearum during pathogenic vs. saprophytic growth, or when infecting different cereal hosts or different tissues of the same host, have been studied. In addition, comparative transcriptomic analyses of F. graminearum knock-out mutants with altered virulence have provided new insights into pathogenicity-related processes. The F. graminearum transcriptomic data generated over the years are now being exploited to build a systems level understanding of the biology of this pathogen, with an ultimate aim of developing effective and sustainable disease prevention strategies.

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Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Transcriptomics of cereal–Fusarium graminearum interactions: what we have learned so far

Kazan

Gardiner

2017

Molecular Plant Pathology

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“…The transcriptomic profile of infected Arabidopsis pistils detected the up-regulation of genes encoding phytoalexin synthesis enzymes of the Brassicaceae-specific camalexin pathway (Su et al, 2011;Brewer and Hammond-Kosack, 2015) and other enzymes of the Trp secondary metabolic pathways important for the synthesis of additional antifungal substances (Bak and Feyereisen, 2001;Bednarek et al, 2009). The results of our analysis allow comparison of phytoalexin production pathways in pistils with those triggered by infection in leaves and roots, where most studies have been carried out.…”

Section: The Transcriptomics Of Arabidopsis Pistil Infection Providesmentioning

confidence: 99%

“…Infection by F. graminearum is facilitated at anthesis by conidia or ascospores attaching to pollen and anthers, from which mycelia colonize the soft tissue of stigmas and ovaries (Miller et al, 2004). Similarly, F. graminearum successfully infects flowers of the model plant species A. thaliana, thus advancing a useful translational model for more rapid studies of host-pathogen interactions (Brewer and Hammond-Kosack, 2015).…”

mentioning

confidence: 99%

Similarities between Reproductive and Immune Pistil Transcriptomes of Arabidopsis Species

et al. 2017

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ORCID IDs: 0000-0002-9468-6335 (M.M.-P.); 0000-0001-6442-4302 (T.D.).Independent lines of evidence suggest that members from ancient and polymorphic gene families such as defensins and receptor-like kinases mediate intercellular communication during both the immune response and reproduction. Here, we report a large-scale analysis to investigate the extent of overlap between these processes by comparing differentially expressed genes (DEGs) in the pistil transcriptomes of Arabidopsis thaliana and Arabidopsis halleri during self-pollination and interspecific pollination and during infection with Fusarium graminearum. In both Arabidopsis species, the largest number of DEGs was identified in infected pistils, where genes encoding regulators of cell division and development were most frequently downregulated. Comparison of DEGs between infection and various pollination conditions showed that up to 79% of down-regulated genes are shared between conditions and include especially defensin-like genes. Interspecific pollination of A. thaliana significantly up-regulated thionins and defensins. The significant overrepresentation of similar groups of DEGs in the transcriptomes of reproductive and immune responses of the pistil makes it a prime system in which to study the consequences of plantpathogen interactions on fertility and the evolution of intercellular communication in pollination.

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“…Arabidopsis thaliana is an exquisite model plant system with numerous genetic and molecular tools available (Somerville & Koornneef, 2002;Koornneef & Meinke, 2010;Provart et al, 2015). Moreover, A. thaliana has been extensively used in the study of plant-pathogen interactions, being critical for progress on understanding the plant immune system (Nishimura & Dangl, 2010;Brewer & Hammond-Kosack, 2015;Provart et al, 2015). However, while most studies focus on the interactions between pathogens and plants, less is known about their interaction with endophytic mutualistic fungi (Tkacz & Poole, 2015).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis thalianaroot colonization by the nematophagous fungusPochonia chlamydosporiais modulated by jasmonate signaling and leads to accelerated flowering and improved yield

et al. 2016

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SummaryPochonia chlamydosporia has been intensively studied in nematode control of different crops. We have investigated the interaction between P. chlamydosporia and the model system Arabidopsis thaliana under laboratory conditions in the absence of nematodes.This study demonstrates that P. chlamydosporia colonizes A. thaliana. Root colonization monitored with green fluorescent protein-tagged P. chlamydosporia and quantitative PCR (qPCR) quantitation methods revealed root cell invasion. Fungal inoculation reduced flowering time and stimulated plant growth, as determined by total FW increase, faster development of inflorescences and siliques, and a higher yield in terms of seed production per plant.Precocious flowering was associated with significant expression changes in key floweringtime genes. In addition, we also provided molecular and genetic evidence that point towards jasmonate signaling as an important factor to modulate progression of plant colonization by the fungus.Our results indicate that P. chlamydosporia provides benefits to the plant in addition to its nematophagous activity. This report highlights the potential of P. chlamydosporia to improve yield in economically important crops.

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Host to a Stranger: Arabidopsis and Fusarium Ear Blight

Cited by 21 publications

References 87 publications

Transcriptomics of cereal–Fusarium graminearum interactions: what we have learned so far

Transcriptomics of cereal–Fusarium graminearum interactions: what we have learned so far

Similarities between Reproductive and Immune Pistil Transcriptomes of Arabidopsis Species

Arabidopsis thalianaroot colonization by the nematophagous fungusPochonia chlamydosporiais modulated by jasmonate signaling and leads to accelerated flowering and improved yield

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