Host Transcription Factors in the Immediate Pro-Inflammatory Response to the Parasitic Mite Psoroptes ovis

Burgess, Stewart T. G.; McNeilly, Tom N.; Watkins, Craig; Nisbet, Alasdair J.; Huntley, John F.

doi:10.1371/journal.pone.0024402

Cited by 19 publications

(12 citation statements)

References 67 publications

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“…Several genes, such as fos , nr4a1 , dusp1 , and nfkbia , which increased after acupuncture needling and are part of the immediate early genes, 6 respond to various external stimulations and trigger the expression of stress response genes. Among them, nfkbia is categorized in the B and T cell receptor pathways and is involved in local immunomodulation.…”

Section: Discussionmentioning

confidence: 99%

From Peripheral to Central: The Role of ERK Signaling Pathway in Acupuncture Analgesia

Park

Park²,

Jeon

et al. 2014

The Journal of Pain

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Despite accumulating evidence of the clinical effectiveness of acupuncture, its mechanism remains largely unclear. We assume that molecular signaling around the acupuncture needled area is essential for initiating the effect of acupuncture. To determine possible bio-candidates involved in the mechanisms of acupuncture and investigate the role of such bio-candidates in the analgesic effects of acupuncture, we conducted 2 stepwise experiments. First, a genome-wide microarray of the isolated skin layer at the GB34-equivalent acupoint of C57BL/6 mice 1 hour after acupuncture found that a total of 236 genes had changed and that extracellular signal–regulated kinase (ERK) activation was the most prominent bio-candidate. Second, in mouse pain models using formalin and complete Freund adjuvant, we found that acupuncture attenuated the nociceptive behavior and the mechanical allodynia; these effects were blocked when ERK cascade was interrupted by the mitogen-activated protein kinase kinase (MEK)/mitogen-activated protein kinase (MAPK) inhibitor U0126 (.8 μg/μL). Based on these results, we suggest that ERK phosphorylation following acupuncture needling is a biochemical hallmark initiating the effect of acupuncture including analgesia. Perspective This article presents the novel evidence of the local molecular signaling in acupuncture analgesia by demonstrating that ERK activation in the skin layer contributes to the analgesic effect of acupuncture in a mouse pain model. This work improves our understanding of the scientific basis underlying acupuncture analgesia.

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Section: Discussionmentioning

confidence: 99%

From Peripheral to Central: The Role of ERK Signaling Pathway in Acupuncture Analgesia

Park

Park²,

Jeon

et al. 2014

The Journal of Pain

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“…In general, microscopic examination of the skin from infested ruminants reveals an increased dermal thickness and a superficial, perivascular dermatitis characterised by a fast influx of lymphocytes (including γδ T-cells in sheep), macrophages, mast cells, neutrophils and eosinophils [ 9 – 12 ]. Previous work on sheep scab demonstrated an up-regulation of genes encoding for pro-inflammatory and pro-allergic mediators [interleukin (IL)-1, IL-8, IL-6] and molecules involved in extravasation of immune cells [ 13 , 14 ]. Several infectious diseases, including scabies, have been linked to CD4+ T-helper cell (Th) differentiation into specific Th1, Th2, Th17 or regulatory (Treg) lineages [ 15 ] and the pro-inflammatory response in the skin of P. ovis infested sheep evolves towards a Th2 immune response within 24 h after infection [ 13 , 14 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…Previous work on sheep scab demonstrated an up-regulation of genes encoding for pro-inflammatory and pro-allergic mediators [interleukin (IL)-1, IL-8, IL-6] and molecules involved in extravasation of immune cells [ 13 , 14 ]. Several infectious diseases, including scabies, have been linked to CD4+ T-helper cell (Th) differentiation into specific Th1, Th2, Th17 or regulatory (Treg) lineages [ 15 ] and the pro-inflammatory response in the skin of P. ovis infested sheep evolves towards a Th2 immune response within 24 h after infection [ 13 , 14 , 16 ]. A similar pattern has been described in Sarcoptes scabiei infested mice and dogs with transcription of Th2 like cytokine profiles in lymph node cells and PBMC respectively [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative immune responses against Psoroptes ovis in two cattle breeds with different susceptibility to mange

Sarre

González-Hernández

Coppernolle

et al. 2015

Vet Res

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The sheep scab mite, Psoroptes ovis, is a major problem in the beef cattle industry, especially in Belgian Blue (BB) cattle. This breed is naturally more predisposed to psoroptic mange but reasons for this high susceptibility remain unknown. Different immune responses could be a potential cause; thus in this study, the cutaneous immune response and in vitro cellular immune response after antigen re-stimulation were examined in naturally infested BB. Cytokine production in the skin and in circulating re-stimulated peripheral blood mononuclear cells (PBMC) demonstrated a mixed pro-inflammatory Th2/Th17 profile, with transcription of IL-4, IL-13, IL-6 and IL-17. Strong IL-17 up-regulation in the skin of BB was associated with an influx of eosinophils and other immune cells, potentially leading towards more severe symptoms. Virtually no changes in cutaneous IFN-γ transcription were detected, while there was substantial IFN-γ up-regulation in re-stimulated PBMC from infested and uninfested animals, potentially indicating a role of this pro-inflammatory cytokine in the innate immune response. In Holstein–Friesian (HF) cattle, generally more resistant to P. ovis infection, a largely similar immunologic response was observed. Differences between HF and BB were the lack of cutaneous IL-17 response in infested HF and low transcription levels of IFN-γ and high IL-10 transcription in re-stimulated PBMC from both infested and uninfested animals. Further research is needed to identify potential cell sources and biological functions for these cytokines and to fully unravel the basis of this different breed susceptibility to P. ovis.

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“…Besides, NJ analysis revealed that PsoSP1 yielded a close relationship with Der f 27. In combination with the homology and the genetic relationship between Pso SP1 and Der f 27, PsoSP1 may be considered as an allergen of P. ovis, which was possibly associated with the instigation of the host cutaneous pro-in ammatory response [23]. Additionally, this cutaneous in ammation resulted in serum extravasation to provide su cient food for mites population growth and cause aggravation of scabby lesions [7,24].…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Serodiagnostic Potential of Two Serpin Proteins in Psoroptes Ovis

Chen

Zhang

et al. 2020

Preprint

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Background: Psoroptes ovis a common ectoparasite of wild and domestic animals, leads to cutaneous inflammation, extreme pruritus, scaly lesions, and causes an economically devastating loss of animal husbandry and animal welfare issues. Serine proteinase inhibitor (Serpin) is present in almost all organisms that are involved in host-pathogen interactions, inflammatory responses, and reproductive development, etc. However, the research on P. ovis serpins is still limited.Methods: In this study, two serpins of P. ovis (PsoSP1 and PsoSP2) were cloned, and the molecular characterization was analyzed by bioinformatics. The transcriptional profiles and tissue localization of PsoSP1 and PsoSP2 in P. ovis were investigated by quantitative real-time PCR and immunohistochemistry, respectively. The potential function of recombinant PsoSP1 and PsoSP2 (rPsoSP1 and rPsoSP2) in the serodiagnosis of P. ovis infestation in rabbits were evaluated by indirect enzyme-linked immunosorbent assay (iELISA). Results: Both of the 523 residue PsoSP1 and the 240 residue PsoSP2 proteins contained typical serpin domains and signatures. Both PsoSP1 and PsoSP2 expressed throughout the life cycle, more specifically, significantly higher expression in female mites than the larva, nymph, and male mites (p < 0.001). The native PsoSP1 and PsoSP2 proteins localized in ovary and mouthpart of adult female mites, respectively. Compared to rPsoSP2, the rPsoSP1 displayed better diagnostic efficiency with higher values of sensitivity, specificity and the area under the receiver operating characteristic curve (AUC) than rPsoSP1 by iELISA (rPsoSP1 - rPsoSP2: 96.0% - 90.0%; 90.91% - 78.18%; 0.988 - 0.964, respectively). Moreover, the rPsoSP1 showed seropositive in 80% rabbits as early as the 2nd week post-infestation (p.i.), prior to visible clinical signs and microscopy-positive of skin scrapings. Conclusions: These results suggested that these two serpins may play essential roles in reproductive development, blood-feeding, and pathogenicity of P. ovis. Compared to PsoSP2, PsoSP1 appeared as a potential antigen for serodiagnosis of P. ovis infestation in rabbits, especially at the early stage of infestation.

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Host Transcription Factors in the Immediate Pro-Inflammatory Response to the Parasitic Mite Psoroptes ovis

Cited by 19 publications

References 67 publications

From Peripheral to Central: The Role of ERK Signaling Pathway in Acupuncture Analgesia

From Peripheral to Central: The Role of ERK Signaling Pathway in Acupuncture Analgesia

Comparative immune responses against Psoroptes ovis in two cattle breeds with different susceptibility to mange

Molecular Characterization and Serodiagnostic Potential of Two Serpin Proteins in Psoroptes Ovis

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