Hot melt extruded polysaccharide blends for controlled drug delivery

Abstract

Different types of hot melt extrudates were prepared based on a variety of blends of ethylcellulose with a 2 nd polysaccharide, namely hydroxypropyl methylcellulose (HPMC), pectin, maize starch, inulin, maltodextrin, guar gum, and chitosan. In selected cases, the

In vitro and in vivo evaluation of a pH-, microbiota- and time-based oral delivery platform for colonic release

“…For reasons of comparison, drug release was also measured in CM free of feces. CM was prepared by dissolving 1.5 g beef extract, 3 g yeast extract, 5 g tryptone, 2.5 g NaCl and 0.3 g L-cysteine hydrochloride in 1 L distilled water (pH 6.5±0.2) [23][24][25]. The resulting solution was sterilized in an autoclave, followed by incubation with the fecal samples under anaerobic conditions at 37±0.…”

Guar gum as a microbially degradable component for an oral colon delivery system based on a combination strategy: formulation and in vitro evaluation

“…The day of the experiment, 3 frozen aliquots were allowed to thaw at room temperature before being added each to a flask containing 90 mL of culture medium (CM) to give simulated colonic fluid. CM was prepared by dissolving 1.5 g of beef extract, 3 g of yeast extract, 5 g of tryptone, 2.5 g of NaCl, 0.3 g L-cysteine hydrochloride in 1 L of deionized water and sterilized by autoclaving at 121 °C for 15 min [9,15,31]. The 3 flasks were placed in a sealed plastic bag with an anaerobic gas generator sachet (AnaeroGen TM , ThermoFisher Scientific, Massachusetts, United States) and incubated for 24 h at 37 °C under horizontal shaking (50 rpm).…”

Oral colon delivery platform based on a novel combination approach: Design concept and preliminary evaluation

“…In vitro drug release in simulated colonic fluid: Coated tablets and capsules were first exposed to 0.1 N HCl for 2 h, followed by 2 h in phosphate buffer pH 4.5 using the USP 43 dissolution paddle apparatus (100 rpm, 800 mL) and a BioDis dissolution tester (Varian, Paris, France; 10 dpm, 200 mL), respectively. The systems were then transferred into 120 mL flasks containing culture medium (CM) inoculated with fecal samples collected from IBD patients as "simulated colonic fluid (SCF)" [26,[29][30][31]. For reasons of comparison, drug release was also measured in pure culture medium.…”