Hot-Water Extracts from Roots of <i>Vitis thunbergii</i> var. <i>taiwaniana</i> and Identified ε-Viniferin Improve Obesity in High-Fat Diet-Induced Mice

Lu, Yeh Lin; Lin, Shyr Yi; Fang, Sheng; Hsieh, Ying Ying; Chen, Chiy Rong; Wen, Chi Luan; Chang, Chi I.; Hou, Wen Chi

doi:10.1021/acs.jafc.7b00327

Cited by 14 publications

(14 citation statements)

References 43 publications

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“…In other studies, ε-viniferin decreased the accumulation of lipids and inhibited of the enzyme HMG-CoA reductase, an enzyme involved in cholesterol synthesis, for a concentration range of 2.5 to 50 µM in 3T3-L1 adipocytes [ 12 , 13 ]. Thus, despite the weak adipose concentrations of ε-viniferin observed in our study as compared to those reported to exert an anti-adipogenic effect in vitro, we believe they are sufficient to induce an anti-adipogenic effect in vivo.…”

Section: Discussionmentioning

confidence: 98%

“…Moreover, studies mentioned above also reported that an oral administration of ε-viniferin was found to limit weight gain in mice fed a hypercaloric diet without decreasing their food intake. The authors explained the weight loss by a decrease in the mass of mesenteric white adipose tissue [ 12 , 13 ]. This suggests that the rage of concentrations found in adipose tissue in our study may be responsible for the effect observed in studies in mice.…”

Section: Discussionmentioning

confidence: 99%

“…These results are in accordance with a study in which, ε-viniferin (2.5, 5, and 10 µM) decreased the size of lipid deposits in vitro. It also reduced the body weight and the weight ratio of mesenteric fat in mice (10 and 25 mg/kg/day, 5 weeks) [ 13 ]. These findings on the potential anti-obesity properties of ε-viniferin are opening a new and interesting line of research that has received little attention to date.…”

Section: Introductionmentioning

confidence: 99%

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Tissular Distribution and Metabolism of trans-ε-Viniferin after Intraperitoneal Injection in Rat

et al. 2018

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Background: Recent studies showed that trans-ε-viniferin (ε-viniferin), a trans-resveratrol dehydrodimer, has anti-inflammatory and anti-obesity effects in rodents. The main purpose of this work was to assess the tissue distribution study of ε-viniferin and its metabolites after intraperitoneal (IP) administration in rat. Methods: After IP injection of 50 mg/kg, ε-viniferin and its metabolites were identified and quantified in plasma, liver, kidneys, adipose tissues, urine, and faeces by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS). Results: ε-Viniferin underwent a rapid hepatic metabolism mostly to glucuronides but also to a lesser extent to sulphate derivatives. The highest glucuronide concentrations were found in liver followed by plasma and kidneys whereas only traces amounts were found in adipose tissues. In contrast the highest ε-viniferin areas under concentration (AUC) and mean residence times (MRT) values were found in white adipose tissues. Finally, much lower levels of ε-viniferin or its metabolites were found in urine than in faeces, suggesting that biliary excretion is the main elimination pathway. Conclusion: A rapid and large metabolism of ε-viniferin and a high bioaccumulation in white adipose tissues were observed. Thus, these tissues could be a reservoir of the native form of ε-viniferin that could allow its slow release and a sustained presence within the organism.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tissular Distribution and Metabolism of trans-ε-Viniferin after Intraperitoneal Injection in Rat

et al. 2018

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“…The hot water extracts of root parts of Vitis thunbergii var. taiwaniana and the isolated ε‐viniferin, 8 as well as the chloroform‐partitioned fraction of hot‐water extracts of Cucurbita moschata and its isolated dehydrodiconiferyl alcohol, 9 showed anti‐adipogenic effects with respect to reducing lipid accumulations in Oil Red O (ORO)‐stained differentiated 3 T3‐L1 adipocytes.…”

Section: Introductionmentioning

confidence: 99%

Anti‐adipogenic activities of pheophorbide a and pyropheophorbide a isolated from wild bitter gourd (Momordica charantia L. var. abbreviata Seringe) in vitro

Liu

Chang

et al. 2022

J Sci Food Agric

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BACKGROUND: The wild bitter gourd (WBG) is a commonly consumed vegetable in Asia that has antioxidant and hypoglycemic properties. The present study aimed to investigate the anti-adipogenic activities of isolated compounds from WBG on 8-day differentiated cultures of 3 T3-L1 adipocytes that were then stained with Oil Red O (ORO) or diamidino-2-phenylindole (DAPI).RESULTS: ORO stains of the methanol extracts of de-seeded HM86 cultivar of WBG (WBG-M) and the ethyl acetate fractions (WBG-M-EA) showed anti-adipogenic activities against differentiated adipocytes. Two chlorophyll-degraded compounds, pheophorbide a (1) and pyropheophorbide a (2), were isolated from WBG-M-EA. Treatments with 1 (5, 10, and 20 ∼mol L −1 ) and 2 (2.5, 5, and 10 ∼mol L −1 ) showed dose-dependent reductions in lipid accumulations and reduced nuclear DAPI stains in differentiated 3 T3-L1 adipocytes. The concentrations for 50% inhibition against lipid accumulations of 1 and 2, respectively, were 16.05 and 7.04 ∼mol L −1 . Treatments with 1 and 2 showed enhanced lactate dehydrogenase release in the first 4-day cell mitotic clonal expansions during the differentiating cultural processes, although the effect was less on the non-differentiating cultural processes. Thus, 1 and 2 were more toxic to differentiating adipocytes than to non-differentiated pre-adipocytes, which partly resulted in anti-adipogenic activities with lowered lipid accumulations.CONCLUSION: Both 1 and 2 showed anti-adipogenic activities in cell models. These chlorophyll-degraded compounds commonly exist in several vegetables during storage or edible seaweeds, which will provide resources for further investigations aiming to test anti-obesity in animal studies.

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“…Moreover, εVin is also promising for its anti-adipogenic properties insofar as it has been shown that this compound decreased lipid accumulation and adipogenesis marker gene expression such as Peroxisome Proliferatoractivated Receptor gamma (PPAR ϒ ) in a 3T3-L1 adipocytes cell line [29]. The decrease in body weight gain in rat fed with a high fat diet and the reduction of the mass of white adipose tissues in mice after oral administration of εVin [29,30] highlight its potential in vivo biological activities. These interesting properties lead to asking the question of its fate in the systemic circulation after ingestion.…”

Section: Introductionmentioning

confidence: 99%

Trans-ε-Viniferin Encapsulation in Multi-Lamellar Liposomes: Consequences on Pharmacokinetic Parameters, Biodistribution and Glucuronide Formation in Rats

et al. 2021

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Trans-ε-viniferin (εVin) is a resveratrol dimer exhibiting promising biological activities for human health. Its bioavailability being low, the development of encapsulation methods would be used to overcome this issue. The aim of this study was to measure the consequences of the encapsulation of εVin in multilamellar liposomes on its pharmacokinetic parameters, metabolism and tissue distribution in rats. After oral administration of εVin (20 mg/kg body weight), either as free or encapsulated forms, plasmas were sequentially collected (from 0 to 4 h) as well as liver, kidneys and adipose tissues (4 h after administration) and analyzed by LC-HRMS. The glucuronide metabolites (εVG) were also produced by hemisynthesis for their quantification in plasma and tissues. The encapsulation process did not significantly modify the pharmacokinetic parameters of εVin itself. However, a significant increase of the T1/2 was noticed for εVG after administration of the encapsulated form as compared to the free form. An accumulation of εVin and εVG in adipose tissues was noticed, and interestingly a significant increase of the latter in the mesenteric one after administration of the encapsulated form was highlighted. Since adipose tissues could represent storage depots, and encapsulation allows for prolonging the exposure time of glucuronide metabolites in the organism, this could be of interest to promote their potential biological activities.

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Hot-Water Extracts from Roots of Vitis thunbergii var. taiwaniana and Identified ε-Viniferin Improve Obesity in High-Fat Diet-Induced Mice

Cited by 14 publications

References 43 publications

Tissular Distribution and Metabolism of trans-ε-Viniferin after Intraperitoneal Injection in Rat

Tissular Distribution and Metabolism of trans-ε-Viniferin after Intraperitoneal Injection in Rat

Anti‐adipogenic activities of pheophorbide a and pyropheophorbide a isolated from wild bitter gourd (Momordica charantia L. var. abbreviata Seringe) in vitro

Trans-ε-Viniferin Encapsulation in Multi-Lamellar Liposomes: Consequences on Pharmacokinetic Parameters, Biodistribution and Glucuronide Formation in Rats

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