Hotmelt tissue adhesive with supramolecularly-controlled sol-gel transition for preventing postoperative abdominal adhesion

Nishiguchi, Akihiro; Ichimaru, Hiroaki; Ito, Shima; Nagasaka, Kazuhiro; Taguchi, Tetsushi

doi:10.1016/j.actbio.2022.04.037

Cited by 27 publications

(29 citation statements)

References 49 publications

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“…Mesothelin staining (for the mesothelium) showed that the mesothelium covered injured tissue surfaces in TG-PEG-treated samples, indicating that TG-PEG did not interfere with the wound-healing process. Compared to the treatment of the models with Seprafilm, TG-PEG showed almost the same level of anti-adhesive property, formation of mesothelial layers, and wound healing process as Seprafilm. These results suggest that TG-PEG LLPS hydrogels can be used as postoperative barriers to prevent adhesion.…”

Section: Resultsmentioning

confidence: 92%

“…However, the viscosity of the pre-gel solution and the mechanical strength of the hydrogels have a trade-off relationship, particularly in the case of physically crosslinked hydrogels. For example, single-syringe hotmelt tissue adhesives based on a supramolecular chemistry approach suffered from their high viscosity even when warmed up due to hydrogen bonding. Because LLPS structures can reduce viscosity, TG-PEG LLPS hydrogels have the potential to improve the injectability.…”

Section: Resultsmentioning

confidence: 99%

“…Compared to film- and double-syringe-type barriers, LLPS hydrogels are delivered in laparoscopic surgery and do not require specific skills. Although the hotmelt adhesive attached to the tissues at a relatively high temperature (45 °C), the temperature dropped to 38 °C within 30 s, which indicates that the adhesives warmed may not damage the tissues. On the other hand, a delivery applicator with a heating unit of adhesives needs to be newly designed to spray the adhesives, which is not required for other temperature-responsive adhesives .…”

Section: Resultsmentioning

confidence: 99%

“…However, polymer solutions (e.g., icodextrin) that do not form hydrogels have poor retention properties in vivo , which may lead to insufficient clinical evidence to prevent postoperative adhesions. , Although several single-syringe barriers, which change the viscoelasticity in response to external stimuli such as temperature, , biomolecules, and shear stress, − have been studied, these barriers have drawbacks in tissue adhesiveness, mechanical strength, and therapeutic efficacy. We previously reported a single-syringe hotmelt-type hydrogel as a postoperative barrier based on a supramolecular chemistry approach . By tuning the sol–gel transition behavior of gelatin using quadruple hydrogen bonding, tissue-adhesive hotmelt tissue adhesives were developed.…”

Section: Introductionmentioning

confidence: 99%

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Liquid–Liquid Phase-Separated Hydrogel with Tunable Sol–Gel Transition Behavior as a Hotmelt-Adhesive Postoperative Barrier

Nishiguchi

Ito

Nagasaka

et al. 2022

ACS Appl. Bio Mater.

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Postoperative barriers have been widely used to prevent adhesions. However, there are currently few barriers that satisfy clinical requirements, such as tissue adhesion, operability, and biocompatibility. Inspired by the adhesion system of living organisms, we report a liquid–liquid phase-separated hydrogel as a single-syringe hotmelt-type postoperative barrier. Mixing polyethylene glycol with gelatin formed liquid–liquid phase-separated hydrogels through segregative liquid–liquid phase separation. Incorporation of a liquid–liquid phase-separated system into gelatin can enhance the sol–gel transition temperature to give a hotmelt-adhesive property to hydrogels. Hotmelt-adhesive hydrogels became a sol phase and cohered into tissue gaps when warmed and solidified at body temperature to adhere to soft tissues. The hydrogels exhibited tissue adhesion to large intestine tissues and showed improved mechanical strength, gelation time, and shear-thinning properties. In rat cecum-abdominal adhesion models, it was confirmed that the resulting hydrogels prevented abdominal adhesion and did not prevent tissue regeneration. Hotmelt-adhesive hydrogels with high tissue adhesive properties, operability, and biocompatibility have enormous potential as barriers to prevent postoperative complications.

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Section: Resultsmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Liquid–Liquid Phase-Separated Hydrogel with Tunable Sol–Gel Transition Behavior as a Hotmelt-Adhesive Postoperative Barrier

Nishiguchi

Ito

Nagasaka

et al. 2022

ACS Appl. Bio Mater.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, the drastically decreased viscosity of the hydrogel also demonstrated the decreased intermolecular interaction (Fig. 6 b) and increased segment mobility as temperature enhanced [ 14 , 71 ]. The hydrogels could adhere to the surface of the tissues through topological entanglement between the sealants and the micro-surface of the skin tissues due to the increased chain mobility at body temperature (Fig.…”

Section: Resultsmentioning

confidence: 99%

Bioinspired Injectable Self-Healing Hydrogel Sealant with Fault-Tolerant and Repeated Thermo-Responsive Adhesion for Sutureless Post-Wound-Closure and Wound Healing

et al. 2022

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Hydrogels with multifunctionalities, including sufficient bonding strength, injectability and self-healing capacity, responsive-adhesive ability, fault-tolerant and repeated tissue adhesion, are urgently demanded for invasive wound closure and wound healing. Motivated by the adhesive mechanism of mussel and brown algae, bioinspired dynamic bonds cross-linked multifunctional hydrogel adhesive is designed based on sodium alginate (SA), gelatin (GT) and protocatechualdehyde, with ferric ions added, for sutureless post-wound-closure. The dynamic hydrogel cross-linked through Schiff base bond, catechol-Fe coordinate bond and the strong interaction between GT with temperature-dependent phase transition and SA, endows the resulting hydrogel with sufficient mechanical and adhesive strength for efficient wound closure, injectability and self-healing capacity, and repeated closure of reopened wounds. Moreover, the temperature-dependent adhesive properties endowed mispositioning hydrogel to be removed/repositioned, which is conducive for the fault-tolerant adhesion of the hydrogel adhesives during surgery. Besides, the hydrogels present good biocompatibility, near-infrared-assisted photothermal antibacterial activity, antioxidation and repeated thermo-responsive reversible adhesion and good hemostatic effect. The in vivo incision closure evaluation demonstrated their capability to promote the post-wound-closure and wound healing of the incisions, indicating that the developed reversible adhesive hydrogel dressing could serve as versatile tissue sealant.

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A Bio‐Inspired Janus Patch for Treating Abdominal Wall Defects

Liu,

Bai

et al. 2024

Adv Funct Materials

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Implantable biomedical patch used in treating abdominal wall defects requires antibacterial, anti‐adhesion, and pro‐healing activities to ensure surgical success and prevent postsurgical complications. However, it often encounters challenges in fulfilling these three critical properties simultaneously, as these processes can always be contradictory during treatment. Herein, to break the barriers between bactericidal activity and cytocompatibility, cell adhesion and anti‐adhesion, a bio‐inspired patch with an asymmetric Janus structure is developed. This bio‐inspired Janus patch features cicada wing‐inspired nanostructures on its top surface, enabling both mechano–bactericidal effects and promotion of fibroblast adhesion and proliferation, which stems from its inherent “selective biocidal activity” toward bacteria and mammalian cells. Subsequently, a poly‐zwitterion layer with robust nonfouling properties is grafted on the bottom surface, realizing cell adhesion and anti‐adhesion on two sides of the same patch. Compared to the commercially available polypropylene (PP) meshes, the top surface of the Janus patch, which faces the abdominal wall, demonstrates superior capabilities in preventing postoperative infection and promoting tissue repair. Simultaneously, the other side facing the viscera efficiently prevents any visceral tissue adhesion. With these prominent performances, the bio‐inspired Janus patch presents a pioneering strategy for designing next‐generation patches to treat abdominal wall defects.

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Hotmelt tissue adhesive with supramolecularly-controlled sol-gel transition for preventing postoperative abdominal adhesion

Cited by 27 publications

References 49 publications

Liquid–Liquid Phase-Separated Hydrogel with Tunable Sol–Gel Transition Behavior as a Hotmelt-Adhesive Postoperative Barrier

Liquid–Liquid Phase-Separated Hydrogel with Tunable Sol–Gel Transition Behavior as a Hotmelt-Adhesive Postoperative Barrier

Bioinspired Injectable Self-Healing Hydrogel Sealant with Fault-Tolerant and Repeated Thermo-Responsive Adhesion for Sutureless Post-Wound-Closure and Wound Healing

A Bio‐Inspired Janus Patch for Treating Abdominal Wall Defects

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