House dust allergen activates the classical complement pathway in mouse serum

Beukelman, C.J.; Rademaker, P.M.; Dijk, Hans van; Aerts, Piet C.; Berrens, L.; Willers, J. M. N.

doi:10.1016/0165-2478(86)90049-0

Cited by 5 publications

(6 citation statements)

References 19 publications

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“…Based on the dose-response curve for commercial lipopolysaccharide in the adjuvant assay, the activity of the subfractions could be fully explained by endotoxin, while that of fraction Ac could not. With respect to the anti-complementary activity of the three house dust fractions, endotoxin seemed of minor importance which confirms earlier findings dealing with fraction C [3].…”

Section: Discussionsupporting

confidence: 89%

“…fr.C The immunological adjuvant activity of house dust fraction C, a potent activator of the human [6,7,10] and mouse [3] classical complement pathways, was investigated and compared with that of dextran sulphate, the most active adjuvant tested in the asialo-SRBC assay so far [20]. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The initial idea that the most anti-complementary factor and putative adjuvant in fraction C might be identical and involve DNA or DNA-fragments [3] was not sustained. The identity of the complement-dependent adjuvant in fraction Ac remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…In a recent publication [3] it was shown that the most allergenic fraction derived from house dust (fraction C) also activated the mouse classical complement pathway. This implies that the mouse is suitable to investigate the relationship between complement activation by house dust fractions and their adjuvant activity in the allergic response.…”

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confidence: 99%

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House dust extracts contain potent immunological adjuvants

Beukelman

Dijk²,

Aerts

et al. 1987

Immunology Letters

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Section: Discussionsupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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House dust extracts contain potent immunological adjuvants

Beukelman

Dijk²,

Aerts

et al. 1987

Immunology Letters

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“…Human pooled serum (HPS) from healthy volunteers was used as the source of complement. Dextran sulfate (Sigma) was used as positive control [9]. The interference with the individual complement components C1 and C2 was evaluated as described elsewhere [10] and results expressed in percentage of complement activity (A), calculated as A = (complement activity of HPS treated with clionasterol ± complement activity of heat-inactivated HPS treated with clionasterol)/(complement activity of HPS ± complement activity of heat-inactivated HPS) 100.…”

Section: Methodsmentioning

confidence: 99%

Clionasterol: A Potent Inhibitor of Complement Component C1

et al. 2003

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Clionasterol (1a), clionasterol monoacetate (1b) and 5alpha,8alpha-epidioxy-24alpha-ethylcholest-6-en-3-ol (2), isolated from the marine sponge Xestospongia exigua, and beta-sitosterol (3) were tested for their influence on the classical (CP) and alternative (AP) pathways of activation of the human complement system in vitro. All the sterols inhibited the CP in a dose-dependent manner but no detectable effect was observed in the AP even at concentrations of 400 microM. Clionasterol was found to be a potent inhibitor of CP (IC50 = 4.1 microM) being ten-fold more active than beta-sitosterol. The presence of the epidioxy group on C-5 and C-8 of compound 2 caused a pronounced decrease of the inhibitory effect. Mechanistic studies on the anticomplementary effect of clionasterol revealed that it interferes with the complement component C1.

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