House Dust and Storage Mite Extracts Influence Skin Keratinocyte and Fibroblast Function

Arlian, Larry G.; Morgan, Marjorie S.; Peterson, K.T.

doi:10.1159/000107464

Cited by 32 publications

(48 citation statements)

References 64 publications

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“…IL1R is an important mediator involved in many cytokine-induced immune and inflammatory responses. Moreover, overexpression of IL1R in both keratinocytes and fibroblasts has been shown to influence the pathophysiology of atopic dermatitis of skin (Arlian et al, 2008). H 2 O 2 treatment strongly enhanced the expression of IL1R, in accordance with previous studies (Higgins et al, 1999).…”

Section: Nannog Positively Regulated Genes Involves In Skin Texture Asupporting

confidence: 90%

Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

Letsiou¹,

Kalliampakou

Gardikis³

et al. 2017

Front. Mar. Sci.

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Nowadays, there is huge interest in natural products obtained from marine organisms that can promote a state of health and well-being for humans. Microalgae represent a primary source of bioactive compounds that could be used as functional ingredients in cosmetic formulations. The aim of the present study is to evaluate, for the first time, the effects of Nannochloropsis gaditana extract against oxidative stress in human primary fibroblasts so as to investigate the potential applications of it in cosmetics. To gain an insight into the molecular mechanisms of N. gaditana bioactivity, we developed a new RT-qPCR platform for studying transcript accumulation for an array of selected genes (up to 100) involved in many skin-related processes including anti-aging, hydration, oxidative stress response, and DNA damage. For the oxidative stress evaluation, H 2 O 2 was used as a stressor. The study of the transcript accumulation of genes revealed that N. gaditana extract exhibits skin protection properties by mediating oxidative responses and apoptosis (including SOD1, GPX1, BID), positively regulates genes involves in skin texture and hydration (including AQP3, Col6A1, FBN1) and modulates the expression of genes involved in skin irritation, DNA damage and aging (including IL1R, PCNA, FOXO3). These findings indicate that the specific N. gaditana extract possesses significant in vitro skin protection activity against induced oxidative stress, and provide new insights into the beneficial role of microalgae bioactive compounds in cosmetic formulations protecting skin from oxidative stress.

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Section: Nannog Positively Regulated Genes Involves In Skin Texture Asupporting

confidence: 90%

Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

Letsiou¹,

Kalliampakou

Gardikis³

et al. 2017

Front. Mar. Sci.

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“…No differences were detected at baseline between the two groups; however, increased IL-8 and KC-like secretion levels were seen in atopic compared with healthy keratinocytes after stimulation. These data are in agreement with previous studies showing an increased production of proinflammatory cytokines by HDM (7,(22)(23)(24)(25). The different behaviours between the two keratinocyte populations suggest that atopic keratinocytes could respond excessively to environmental stimuli and that intrinsic alterations present in atopic keratinocytes, and not the presence of external proteases, may contribute to the excessive inflammatory response in AD.…”

Section: Discussionsupporting

confidence: 94%

Evaluation of antimicrobial peptides and cytokine production in primary keratinocyte cell culture from healthy and atopic beagles

Santoro

Ahrens

Marsella

et al. 2015

Experimental Dermatology

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Increased secretion of antimicrobial peptides and cytokines is present in atopic skin. The purpose of this study was to compare the production of b-defensin (cBD)3-like, cathelicidin (cCath) and cytokines in atopic and healthy canine keratinocytes. Seven atopic house dust mites (HDMs) sensitive and five healthy age-matched beagles were used. Keratinocytes were stimulated for 24 h, and the supernatant was collected. A significantly higher production of cBD3-like was present at baseline in atopic compared with healthy keratinocytes, but cBD3-like did not increase after stimulation. IL-17 and lipopolysaccharide increased cBD3-like in healthy compared with atopic keratinocytes. cCath increased in both groups after stimulation. Atopic keratinocytes exposed to HDM produced more IL-8 and keratinocyte-derived chemokine-like than healthy keratinocytes. Exposure to HDM induced an increased IL-8 in atopic keratinocytes and a decreased IFN-c in healthy keratinocytes. These results may suggest an over sensitization of atopic keratinocytes and a possible impairment of the cutaneous defense against micro-organisms.

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“…Stimulated keratinocytes and fibroblasts produce and secrete multiple cytokines that promote cutaneous inflammation. Previous research has shown that house dust mite and stored product mite extracts stimulate the secretion of cytokines from cultured human dermal keratinocytes, fibroblasts and microvascular endothelial cells [20,21]. Both D. farinae and D. pteronyssinus extracts stimulate cultured keratinocytes to increase secretion of the chemokine growth-related oncogene-α (GROα, CXCL1), which is chemotactic for the extravasation of leukocytes during inflammation [20].…”

Section: Introductionmentioning

confidence: 99%

“…Previous research has shown that house dust mite and stored product mite extracts stimulate the secretion of cytokines from cultured human dermal keratinocytes, fibroblasts and microvascular endothelial cells [20,21]. Both D. farinae and D. pteronyssinus extracts stimulate cultured keratinocytes to increase secretion of the chemokine growth-related oncogene-α (GROα, CXCL1), which is chemotactic for the extravasation of leukocytes during inflammation [20]. Likewise, D. farinae and/or D. pteronyssinus extracts induce cultured normal human dermal fibroblasts to secrete interleukin (IL)-6, IL-8 (CXCL8), monocyte chemoattractant protein-1 (MCP-1, CCL2) and macrophage colony-stimulating factor (M-CSF) [20].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulation of Skin Cytokine Secretion by House Dust Mite Extracts

Arlian

Morgan²

2011

Int Arch Allergy Immunol

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Background: Skin contact with house dust mites may contribute to atopic dermatitis and other skin diseases. We sought to determine if molecules from house dust mites could influence the release of proinflammatory cytokines and chemokines from epidermal keratinocytes and dermal fibroblasts grown in a human skin equivalent (HSE) model. Methods: HSEs consisting of an epidermis of keratinocytes with stratum corneum over a dermis of fibroblasts in a collagen matrix were challenged with Dermatophagoides farinae, D. pteronyssinus and Euroglyphus maynei mite extracts. Results: HSEs secreted interleukin (IL)-1α, IL-1 receptor antagonist, IL-6, IL-8, cutaneous T cell-attracting chemokine, transforming growth factor-α, granulocyte/macrophage and macrophage colony-stimulating factors and vascular endothelial cell growth factor in response to at least 1 mite extract. Extracts of different mite species stimulated HSEs to release different cytokines. Therefore, extracts of different species contained different molecules or different concentrations of similar molecules. The cytokine release profiles of cells in the HSEs were not the same as for monocultured keratinocytes and fibroblasts. Conclusions: Molecules from house dust mites are capable of inducing the release of multiple proinflammatory cytokines and chemokines from epidermal keratinocytes and dermal fibroblasts. Avoiding skin contact with house dust mites would reduce the possibility of mite-induced inflammation in the skin. Therefore, measures to reduce contact with mite molecules such as frequent vacuuming of upholstered furniture and carpets and laundering of clothing and bedding to remove mite molecules and allergens could reduce skin contact with mite molecules and diminish exacerbations of skin inflammation in patients with atopic dermatitis and other skin diseases.

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House Dust and Storage Mite Extracts Influence Skin Keratinocyte and Fibroblast Function

Cited by 32 publications

References 64 publications

Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

Evaluation of antimicrobial peptides and cytokine production in primary keratinocyte cell culture from healthy and atopic beagles

Immunomodulation of Skin Cytokine Secretion by House Dust Mite Extracts

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