House dust mite extract forms a der p 2 corona on multi-walled carbon nanotubes: implications for allergic airway disease

Dominguez, Judith; Holmes, Samantha K.; Bartone, Ryan D.; Tisch, Logan J.; Tighe, Robert M.; Bonner, James C.; Payne, Christine K.

doi:10.1039/d3en00666b

Environ. Sci.: Nano

2024

DOI: 10.1039/d3en00666b

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House dust mite extract forms a der p 2 corona on multi-walled carbon nanotubes: implications for allergic airway disease

Judith Dominguez,

Samantha K. Holmes,

Ryan D. Bartone

et al.

Abstract: MWCNTs are used in a variety of industrial applications. Inhalation of MWCNTs can occur during manufacturing and handling. We address the interaction of MWCNTs with house dust mite allergens (a source of asthma) and lung fluid proteins.

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House Dust Mite Proteins Adsorb on Multiwalled Carbon Nanotubes Forming an Allergen Corona That Intensifies Allergic Lung Disease in Mice

Bartone,

Tisch,

Dominguez

et al. 2024

ACS Nano

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The increasing use of multiwalled carbon nanotubes (MWCNTs) could increase the risk of allergic lung disease in occupational or consumer settings. We previously reported that MWCNTs exacerbated allergic lung disease in mice induced by extract from house dust mites (HDM), a common cause of asthma in humans. Because MWCNTs avidly bind biomolecules to form protein coronas that can modify immunotoxicity, we hypothesized that exacerbation of allergic lung disease in mice caused by coexposure to MWCNTs and HDM extract was due to the formation of an allergen corona. In a first set of experiments, male and female C57BL/6J mice were coexposed to MWCNTs and HDM extract over 3 weeks compared to MWCNTs or HDM extract alone. In a second set of experiments, mice were exposed to pristine MWCNTs or MWCNTs with an HDM allergen corona (HDM-MWCNTs). HDM-MWCNTs were formed by incubating MWCNTs with HDM extract, where ∼7% of proteins adsorbed to MWCNTs, including Der p 1 and Der p 2. At necropsy, bronchoalveolar lavage fluid was collected from lungs to assess lactate dehydrogenase, total protein and inflammatory cells, while lung tissue was used for histopathology, qPCR, and Western blotting. Compared to MWCNTs or HDM extract alone, coexposure to MWCNTs and HDM extract or exposure to HDM-MWCNTs increased pathological outcomes associated with allergic lung disease (eosinophilia, fibrosis, mucous cell metaplasia), increased mRNAs associated with fibrosis ( Col1A1 , Arg1 ) and enhanced STAT6 phosphorylation in lung tissue. These findings indicated that exacerbation of HDM-induced allergic lung disease by MWCNTs is due to an allergen corona.

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House Dust Mite Proteins Adsorb on Multiwalled Carbon Nanotubes Forming an Allergen Corona That Intensifies Allergic Lung Disease in Mice

Bartone,

Tisch,

Dominguez

et al. 2024

ACS Nano

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House dust mite extract forms a der p 2 corona on multi-walled carbon nanotubes: implications for allergic airway disease

Abstract: MWCNTs are used in a variety of industrial applications. Inhalation of MWCNTs can occur during manufacturing and handling. We address the interaction of MWCNTs with house dust mite allergens (a source of asthma) and lung fluid proteins.

Cited by 1 publication

References 69 publications

House Dust Mite Proteins Adsorb on Multiwalled Carbon Nanotubes Forming an Allergen Corona That Intensifies Allergic Lung Disease in Mice

House Dust Mite Proteins Adsorb on Multiwalled Carbon Nanotubes Forming an Allergen Corona That Intensifies Allergic Lung Disease in Mice

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