Housefly Pupae-Derived Antioxidant Peptides Exerting Neuroprotective Effects on Hydrogen Peroxide-Induced Oxidative Damage in PC12 Cells

Sun, Tingting; Zhang, Sichen; Yang, Wenzhe; Zhao, Zhimin; Yang, Dan

doi:10.3390/molecules24244486

Cited by 16 publications

(10 citation statements)

References 42 publications

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“…In another paper, two antioxidant peptides were obtained from the Alcalase hydrolysate of Arca subcrenata [373]. In another study, two antioxidant peptides (DFTPVCTTELGR and ARFEELCSDLFR) were purified from the Alcalase hydrolysate of housefly (Musca domestica L.) pupae [374]. They exhibited strong ABTS and cation radical scavenging activity with EC50 values of 0.39 and 0.35 mM, respectively [374].…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

Use of Alcalase in the production of bioactive peptides: A review

Tacias-Pascacio

Morellon‐Sterling

Siar

et al. 2020

International Journal of Biological Macromolecules

222

114

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Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

Use of Alcalase in the production of bioactive peptides: A review

Tacias-Pascacio

Morellon‐Sterling

Siar

et al. 2020

International Journal of Biological Macromolecules

222

114

View full text Add to dashboard Cite

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“…pupae. The active peptides can effectively protect PC12 cells from oxidative damage induced by hydrogen peroxide by decreasing intracellular reactive oxygen species and malonaldehyde recovering cellular mitochondrial membrane potential and increasing the activity of intracellular superoxide dismutase guided by ABTS cation radical scavenging activity [ 34 ]. An antioxidant peptide derived from walnut meal protein and passed through gastrointestinal digestion displayed ABTS radical scavenging activity and ORAC assay also exhibited significantly protective effect on SH-SY5Y cells against oxidative damage [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Hydrolase-Treated Royal Jelly Attenuates H2O2- and Glutamate-Induced SH-SY5Y Cell Damage and Promotes Cognitive Enhancement in a Rat Model of Vascular Dementia

Sirinupong

Chansuwan

Kaewkaen

2021

International Journal of Food Science

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Vascular dementia (VaD) is the second most common type of dementia following Alzheimer’s disease, but the therapeutic efficacy is still not effective. This makes the searching for novel neuroprotective agents important. Therefore, we hypothesized that royal jelly, a well-known traditional medicine, could attenuate memory impairment and brain damage in vascular dementia. This study determined the effects of royal jelly hydrolysate (RJH) and possible mechanism of cell damage and cognitive-enhancing effect in animal study. An in vitro study assessed the effects of RJH on acetylcholinesterase inhibitor, cell viability, and cell damage in SH-SY5Y neuroblastoma cells. Then, an in vivo study examined vascular dementia by the occlusion of the right middle cerebral artery (Rt.MCAO); adult male Wistar rats had been orally given RJH at doses ranging from 10, 50, to 100 mg/kg for 14 days before and 14 days after the occlusion of Rt.MCAO to mimic the VaD condition. Rats’ spatial memory was evaluated using Morris water maze and radial arm maze every 7 days after Rt.MCAO throughout a 14-day experimental period, and then, they were sacrificed and the acetylcholinesterase (AChE) activity in the hippocampus was determined. The results showed that RJH has no cytotoxic effect with the final concentration up to 500 μg protein/ml and reduces cell death from the H2O2- and glutamate-induced cell damage in in vitro neuroblastoma cells. Importantly, RJH significantly improved memory performance in Morris water maze test and radial arm maze and decreased the level of acetyl cholinesterase activity. In conclusion, RJH is the potential neuroprotective agent and cognitive enhancer for VaD.

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“…It is conjectured that fraction F4 with a lower molecular weight can more easily react with free radicals, converting them to more stable products and terminating the radical chain reactions. 25 Fig. 1b-d shows that fraction F4 displayed the best radical scavenging activity of all the fractions.…”

Section: Isolation and Purication Of The Sspmentioning

confidence: 92%

“…24 The natural antioxidant peptides from plants present benets, such as abundant raw materials, high efficiency, and safety. 25 Increasing evidence has suggested that the peptides found in plants inhibit oxidative stress by scavenging free radicals and increasing intracellular antioxidant enzyme activity and GSH levels. [26][27][28][29][30][31][32] Moreover, Zhang et al conrmed that Se and peptides have a synergistic effect, rendering Seenriched soybean peptides (SSP) desirable antioxidants.…”

Section: Introductionmentioning

confidence: 99%