How can molecular micro-CT imaging revolutionize drug discovery?

Shakeri‐Zadeh, Ali

doi:10.1080/17460441.2019.1623203

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…[1] For instance, the visualization of small-animal disease models has helped to understand disease progression. [2,3] Furthermore, besides developmental biology, [4][5][6] microCT is widely used in drug development, [7] imaging of insects in entomology, [8] and virtual histology. [9][10][11][12][13] The resolution in the micrometer range is usually sufficient to identify structural features such as the vascular system and bone structures, or to examine the interior of whole specimens (i.e., chicken embryos).…”

Section: Doi: 101002/advs202201723mentioning

confidence: 99%

X‐ray Stain Localization with Near‐Field Ptychographic Computed Tomography

et al. 2022

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Although X-ray contrast agents offer specific characteristics in terms of targeting and attenuation, their accumulation in the tissue on a cellular level is usually not known and difficult to access, as it requires high resolution and sensitivity. Here, quantitative near-field ptychographic X-ray computed tomography is demonstrated to assess the location of X-ray stains at a resolution sufficient to identify intracellular structures by means of a basis material decomposition. On the example of two different X-ray stains, the nonspecific iodine potassium iodide, and eosin Y, which mostly interacts with proteins and peptides in the cell cytoplasm, the distribution of the stains within the cells in murine kidney samples is assessed and compared to unstained samples with similar structural features. Quantitative nanoscopic stain concentrations are in good agreement with dual-energy micro computed tomography measurements, the state-of-the-art modality for material-selective imaging. The presented approach can be applied to a variety of X-ray stains advancing the development of X-ray contrast agents.

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