How cell wall complexity influences saccharification efficiency in<i>Miscanthus sinensis</i>

Souza, Amanda P. De; Kamei, Claire Lessa Alvim; Torres, Andrés F.; Pattathil, Sivakumar; Hahn, Michael G.; Trindade, Luisa M.; Buckeridge, Marcos Silveira

doi:10.1093/jxb/erv183

Cited by 69 publications

(63 citation statements)

References 49 publications

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“…) suggest a pectin–lignin association, corroborated by the higher signals for some RG‐I and RG‐1/AG‐related mAbs in these extracts. Pectin–lignin associations have been suggested for several species: M. sinensis (de Souza et al ., ), Populus trichocarpa (DeMartini et al ., ), Panicum virgatum (Shen et al ., ) and Medicago sativa (Wi et al ., ). Abundant MLG epitopes in 4 M KOH PC extracts suggest that all harvests contain proportions of tightly bound MLG, only released after delignification.…”

Section: Discussionmentioning

confidence: 99%

A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin

et al. 2016

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Summary Miscanthus spp. are promising lignocellulosic energy crops, but cell wall recalcitrance to deconstruction still hinders their widespread use as bioenergy and biomaterial feedstocks. Identification of cell wall characteristics desirable for biorefining applications is crucial for lignocellulosic biomass improvement. However, the task of scoring biomass quality is often complicated by the lack of a reference for a given feedstock.A multidimensional cell wall analysis was performed to generate a reference profile for leaf and stem biomass from several miscanthus genotypes harvested at three developmentally distinct time points. A comprehensive suite of 155 monoclonal antibodies was used to monitor changes in distribution, structure and extractability of noncellulosic cell wall matrix glycans.Glycan microarrays complemented with immunohistochemistry elucidated the nature of compositional variation, and in situ distribution of carbohydrate epitopes. Key observations demonstrated that there are crucial differences in miscanthus cell wall glycomes, which may impact biomass amenability to deconstruction.For the first time, variations in miscanthus cell wall glycan components were comprehensively characterized across different harvests, organs and genotypes, to generate a representative reference profile for miscanthus cell wall biomass. Ultimately, this portrait of the miscanthus cell wall will help to steer breeding and genetic engineering strategies for the development of superior energy crops.

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Section: Discussionmentioning

confidence: 99%

A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin

et al. 2016

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“…However, biomass recalcitrance has posed a significant challenge in biofuel production. This is due to the fact that lignin-carbohydrate complexes formed by lignin are intimately interlaced with carbohydrates through ester and ether bonds, preventing the enzymatic hydrolysis of fermentable sugars De Souza et al 2015). Among the various techniques developed for the removal of biomass recalcitrance from lignocellulosic biomass, the engineering of microbial fermentation has been proposed as a cost-efficient, eco-friendly, and sustainable development strategy to reduce the risk to the environment and improve the efficiency of biofuel production (Maki et al 2009;Weber et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Novel Laccase-producing Bacillus sp. A4 and its Application in Miscanthus Degradation

Guo¹,

Lin²,

Wang³

et al. 2017

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Bacillus sp. A4 exhibiting laccase production was isolated from forest soil. Its laccase secreted into a LB medium exhibited a maximum activity of 3.9 U mg -1 protein at the optimal temperature (37 °C) and pH (6.0). The purified laccase of Bacillus sp. A4 demonstrated a low molecular mass of 33 kDa, and its optimal temperature and pH were 40 °C and 4.6, respectively, when using ABTS as a substrate. The activity of the purified laccase was significantly increased in the presence of Cu 2+ , methanol, and ethanol, but it was totally inhibited by L-cysteine. The laccase production of this strain was markedly stimulated when the strain was incubated with 0.5% different lignocellulosic biomasses. The highest activity of laccase (22.6 U mg -1 protein) was obtained in using algal biomass. This new strain efficiently decreased the lignin content of lignocellulose biomasses after 9 d of incubation at 37 °C, especially lignin from grasses. Further analysis showed that, compared to that of all tested biomasses, the new strain was a more efficient decomposer of the lignin of Miscanthus, which exhibited much more lignin loss and cell wall structure destruction in a short span of time. Therefore, the potential use of this strain could be advantageous for using lignin in Miscanthus for industrial processes.

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“…As a promising solution, genetic modification of plant cell walls has been proposed for reducing recalcitrance in bioenergy crops (Torney et al, 2007;Xie and Peng, 2010;Vega-Sánchez and Ronald, 2010). Hence, it becomes essential to identify the main factors of plant cell walls that affect biomass enzymatic digestibility (Zhu et al, 2008;DeMartini et al, 2013;De Souza et al, 2015). Recently, three major wall polymers (cellulose, hemicelluloses and lignin) have been characterized with distinct effects on biomass enzymatic saccharification in plants.…”

Section: Introductionmentioning

confidence: 99%

Ammonium oxalate-extractable uronic acids positively affect biomass enzymatic digestibility by reducing lignocellulose crystallinity in Miscanthus

Wang

Huang

Liu

et al. 2015

Bioresource Technology

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How cell wall complexity influences saccharification efficiency inMiscanthus sinensis

Abstract: HighlightThe manner in which lignin is linked to polysaccharides and the polysaccharide–polysaccharide interactions within cell walls of Miscanthus sinensis are associated with recalcitrance to hydrolysis.

Cited by 69 publications

References 49 publications

A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin

A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin

Characterization of a Novel Laccase-producing Bacillus sp. A4 and its Application in Miscanthus Degradation

Ammonium oxalate-extractable uronic acids positively affect biomass enzymatic digestibility by reducing lignocellulose crystallinity in Miscanthus

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