For potato, diploid hybrid breeding is a novel breeding technique that speeds up the development of new varieties. A consequence of hybrid breeding is the introduction of hybrid true potato seeds as starting material. From these seeds, seedling tubers can be produced in one field season, to use as starting material for a seed or a ware crop in the following year. For breeding purposes as well as for seed crop and ware crop production it is essential to produce seedling tubers of high quality. The production of seedling tubers is a new step in the potato production chain, therefore, we investigated the effect of tuber quality traits on plant development and yield. With similar seedling tuber weight, more eyes per seedling tuber led to more stems per plant. This was compensated by a lower number of tubers per stem resulting in an equal total tuber number and weight per plant at the end of the growing season. A higher seedling tuber weight led to a higher soil cover in the field. Therefore, all hybrids produced a higher total tuber weight per plant when grown from larger seedling tubers than when grown from smaller seedling tubers, while number of eyes and stems per tuber has no effect on final yield when using equal seedling tuber weight.