How Crisp is CRISPR? CRISPR-Cas-mediated crop improvement with special focus on nutritional traits

Devising an Innovative, Genotype-free Transformation Protocol for Recalcitrant Indica Rice Genotypes: Samba Mahsuri and Sundarbans’ Salt-tolerant Indica Rice Cultivars White Getu and Hamilton to Establish an Ingenious Platform for CRISPR/Cas9-mediated Genome Editing

“…In recent past, numerous reports have demonstrated the differential applications of CRISPR/Cas-based genome editing in plants. The genome edited plants have been interrogated employing multiple screening techniques to validate the indels or mutations, thereby incorporating the desired traits [7,8,9,11,12,22,26]. We elucidate the development of transgenic plants in recalcitrant indica rice genotypes via transferring the CRISPR/Cas9 reagents into rice genome employing biolistic approach.…”

“…The past decade has ushered in an era of "Genome Sequencing and Genome Editing" with an upsurge of next-generation sequencing platforms and neoteric plant breeding approaches that has altered the manner in which breeding is performed via comprehending the genetics of the trait to expedite the genetic gains. Advent of the cutting edge CRISPR/Cas9 genome editing technique has revolutionized the strategies for high precision genome editing of plants, speci cally staple food and cash crops [7][8][9][10][11][12]. Thus, CRISPR/Cas9-based rice genome alterations have signi cantly fascinated crop scientists, globally [13][14][15][16][17].…”

CRISPR/Cas9‐mediated homology donor repair base editing confers glyphosate resistance to rice (Oryza sativa L.)

“…We have generated GR rice lines by incorporation of three concatenated mutations (G172A, T173P, and P177S) in the conserved PEPbinding motif of the native EPSPS rice gene. Currently, gene editing technologies, especially the CRISPR-Cas9 system, have emerged as a palpably significant cornerstone in plant research that has ushered in an era of development of genome-edited plants via knockouts (KOs), genetic re-establishments, and insertion mutants (Gao, 2015;Chen et al, 2019;Hua et al, 2019;Kaul et al, 2019;Raman et al, 2019;Kaul et al, 2020a;Kaul et al 2020b;Kaul et al, 2022;Tan et al, 2022). This approach has proved to be more advantageous than transgenics due to its simplicity, efficiency, flexibility, versatility, and biosafety (Mussolino et al, 2011;Jinek et al, 2012;Cong et al, 2013;Shan et al, 2013;Ma et al, 2015;Lee et al, 2016;Gao et al, 2017;Zhang et al, 2021;Wei et al, 2022) and is potent for reconstructing novel traits that may not be possible via molecular breeding for crop improvement.…”

“…SSNs have been employed to generate targeted gene KOs in umpteen crops Li et al, 2019;Chen et al, 2021;De Mori et al, 2021;Galli et al, 2021;McCaw et al, 2021). However, native gene fragment replacement via HDR approach at targeted loci within the plant genome has been a monumental challenge to date with minimal efficiencies (Voytas and Gao, 2014;Kaul et al, 2020a). Li et al (2016) utilized CRISPR-Cas9-mediated NHEJ approach for site-directed modification via introduction of TIPS mutation in OsEPSPS gene.…”

“…We have generated GR rice lines by incorporation of three concatenated mutations (G172A, T173P, and P177S) in the conserved PEP-binding motif of the native EPSPS rice gene. Currently, gene editing technologies, especially the CRISPR-Cas9 system, have emerged as a palpably significant cornerstone in plant research that has ushered in an era of development of genome-edited plants via knockouts (KOs), genetic re-establishments, and insertion mutants ( Gao, 2015 ; Chen et al., 2019 ; Hua et al., 2019 ; Kaul et al., 2019 ; Raman et al., 2019 ; Kaul et al., 2020a ; Kaul et al. 2020b ; Kaul et al., 2022 ; Tan et al., 2022 ).…”