How did correlative atomic force microscopy and super-resolution microscopy evolve in the quest for unravelling enigmas in biology?

Miranda, Adelaide; Gómez‐Varela, Ana I.; Stylianou, Andreas; Hirvonen, L; Sánchez, Humberto; Beule, Pieter De

doi:10.1039/d0nr07203f

Cited by 31 publications

(20 citation statements)

References 189 publications

(226 reference statements)

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“…The domain spacings measured by AFM and STORM are nearly identical (46.4 ± 3.7 nm and 46.8 ± 5.0 nm, respectively) for the same set of samples (i.e., prepared under the same conditions at the same time), providing further evidence that the nanoparticles are incorporated into the matrix and not isolated at the film surface. These cylinder-to-cylinder distances are well within the lateral resolving capabilities of both AFM and SMLM, whose correlative use has been on the rise in biology . More specifically, Cy3-aC′ dots generated on average 30 100 photons per localization event (see Figure S6), which translates to a mean localization uncertainty of 7.2 ± 1.1 nm.…”

Section: Results and Discussionsupporting

confidence: 58%

“…These cylinder-tocylinder distances are well within the lateral resolving capabilities of both AFM and SMLM, 76 whose correlative use has been on the rise in biology. 77 More specifically, Cy3-aC′ dots generated on average 30 100 photons per localization event (see Figure S6), which translates to a mean localization uncertainty of 7.2 ± 1.1 nm. These values are remarkably consistent with those reported for Cy3-srC′ dots, 60 corroborating earlier studies that enhanced fluorescent behavior (over free dyes) is preserved despite the core compositional differences between aC′ and srC′ dots.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Orthogonal Nanoprobes Enabling Two-Color Optical Super-Resolution Microscopy Imaging of the Two Domains of Diblock Copolymer Thin Film Nanocomposites

et al. 2021

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Multicolor optical super-resolution microscopy (OSRM) describes an emerging set of techniques for the specific labeling of distinct constituents of multicomponent systems with compatible optical probes, elucidating proximity relationships from far-field imaging of diffraction-limited features with nanometer-scale resolution. While such approaches are well established in the study of biological systems, their implementation in materials science has been considerably slower. In large part, this gradual adoption is due to the lack of appropriate OSRM probes that, e.g., by facile mixing or surface modification, enable orthogonal labeling of specific nanostructures in the condensed state, rather than in aqueous conditions as with biology. Here, OSRM probes in the form of ultrasmall (diameters <10 nm) aluminosilicate nanoparticles encapsulating different fluorescent dyes are tailored to visualize both nanodomains of polystyrene-block-poly[(allyl glycidyl ether)-co-(ethylene oxide)] (PS-b-P(AGE-co-EO)) diblock copolymer thin films. Careful design of nanoprobe surface chemical properties facilitates either selective compatibilization with the nonpolar PS matrix or preferential reactivity with surface allyl groups of the hydrophilic P(AGE-co-EO) minority block. Stochastic optical reconstruction microscopy (STORM) of the resulting polymer–inorganic nanocomposite thin films shows nanodomain features of the two chemically dissimilar blocks consistent with atomic force microscopy results. This work paves the way for multiplexed OSRM analysis of polymer nanocomposite bulk structures.

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Section: Results and Discussionsupporting

confidence: 58%

Section: ■ Results and Discussionmentioning

confidence: 99%

Orthogonal Nanoprobes Enabling Two-Color Optical Super-Resolution Microscopy Imaging of the Two Domains of Diblock Copolymer Thin Film Nanocomposites

et al. 2021

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“…Microscopes rely on specialized approaches to differentiate compounds of interest from the background. For instance, fluorescence microscopes rely on fluorescent-emitting chemicals and proteins for signal, bright-field microscopes generate contrast by attenuation of transmitted light in dense areas, while atomic force microscopes read out force and topographic measurements based on a sample's surface features [71][72][73][74]. Hence, the contrasting regime should be considered before commencing a project, to ensure the compatibility of chosen visualization schemes with the samples and expected outcomes of the project.…”

Section: Resolution Versus Contrastmentioning

confidence: 99%

A guide into the world of high-resolution 3D imaging: the case of soft X-ray tomography for the life sciences

Okolo

2022

Biochemical Society Transactions

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In the world of bioimaging, every choice made determines the quality and content of the data collected. The choice of imaging techniques for a study could showcase or dampen expected outcomes. Synchrotron radiation is indispensable for biomedical research, driven by the need to see into biological materials and capture intricate biochemical and biophysical details at controlled environments. The same need drives correlative approaches that enable the capture of heterologous but complementary information when studying any one single target subject. Recently, the applicability of one such synchrotron technique in bioimaging, soft X-ray tomography (SXT), facilitates exploratory and basic research and is actively progressing towards filling medical and industrial needs for the rapid screening of biomaterials, reagents and processes of immediate medical significance. Soft X-ray tomography at cryogenic temperatures (cryoSXT) fills the imaging resolution gap between fluorescence microscopy (in the hundreds of nanometers but relatively accessible) and electron microscopy (few nanometers but requires extensive effort and can be difficult to access). CryoSXT currently is accessible, fully documented, can deliver 3D imaging to 25 nm resolution in a high throughput fashion, does not require laborious sample preparation procedures and can be correlated with other imaging techniques. Here, we present the current state of SXT and outline its place within the bioimaging world alongside a guided matrix that aids decision making with regards to the applicability of any given imaging technique to a particular project. Case studies where cryoSXT has facilitated a better understanding of biological processes are highlighted and future directions are discussed.

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“…Based on the progress in engineering and advanced microscopy, correlative techniques could solve these issues. Furthermore, the development of various correlative techniques has reduced the resolution of various techniques by as much as an order of magnitude [71,72]. The current status of correlative methods based on X-ray microscopy is presented in Table 2.…”

Section: Sxcm and Correlative Microscopymentioning

confidence: 99%

Nanometer-Resolution Imaging of Living Cells Using Soft X-ray Contact Microscopy

et al. 2022

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Soft X-ray microscopy is a powerful technique for imaging cells with nanometer resolution in their native state without chemical fixation, staining, or sectioning. The studies performed in several laboratories have demonstrated the potential of applying this technique for imaging the internal structures of intact cells. However, it is currently used mainly on synchrotrons with restricted access. Moreover, the operation of these instruments and the associated sample-preparation protocols require interdisciplinary and highly specialized personnel, limiting their wide application in practice. This is why soft X-ray microscopy is not commonly used in biological laboratories as an imaging tool. Thus, a laboratory-based and user-friendly soft X-ray contact microscope would facilitate the work of biologists. A compact, desk-top laboratory setup for soft X-ray contact microscopy (SXCM) based on a laser-plasma soft X-ray source, which can be used in any biological laboratory, together with several applications for biological imaging, are described. Moreover, the perspectives of the correlation of SXCM with other super-resolution imaging techniques based on the current literature are discussed.

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How did correlative atomic force microscopy and super-resolution microscopy evolve in the quest for unravelling enigmas in biology?

Abstract: This review provides a detailed picture of the innovative efforts to combine atomic force microscopy and different super-resolution microscopy techniques to elucidate biological questions.

Cited by 31 publications

References 189 publications

Orthogonal Nanoprobes Enabling Two-Color Optical Super-Resolution Microscopy Imaging of the Two Domains of Diblock Copolymer Thin Film Nanocomposites

Orthogonal Nanoprobes Enabling Two-Color Optical Super-Resolution Microscopy Imaging of the Two Domains of Diblock Copolymer Thin Film Nanocomposites

A guide into the world of high-resolution 3D imaging: the case of soft X-ray tomography for the life sciences

Nanometer-Resolution Imaging of Living Cells Using Soft X-ray Contact Microscopy

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