In the framework of a collaboration between INAIL and University of Salento, an indoor radon survey in 54 buildings belonging to the UniSalento University (Southeast Italy) was carried out. The monitored buildings differ by type, construction period, materials, etc., and are located in an area with a morphology characterized mainly by marls, calcareous marls, and calcarenites (karst area). The sample of the survey includes 963 rooms at different floors: it consists in rooms mainly located at ground floor (67%), first floor (12%), and below ground (12%). SSNTD passive dosimeters measured the average radon activity concentration for two consecutive semesters (spring/summer and autumn/winter) from which annual radon averages were estimated for each room. The spatial variability within buildings was investigated in terms of variation between floors and among rooms at the same floor. Data analysis provides evidence that the distributions (in terms of arithmetic mean, standard deviation, median, and geometric mean) of indoor radon annual averages at ground floor and at first floor within building are very similar. This highlights that the karstic characteristics of soil and building materials affect radon levels not only below ground and at ground floor, but also at first floor. Moreover, to evaluate the spatial variability of radon among buildings or floors, the analysis of the distribution of coefficient of variation (CV) was carried out: the results show a low spatial variability with median and average values of CVs ≤ 30% both for the whole building and at different floor levels.