How Does Glycation Affect Binding Parameters of the Albumin-Gliclazide System in the Presence of Drugs Commonly Used in Diabetes? In Vitro Spectroscopic Study

Abstract

In this research, the selected drugs commonly used in diabetes and its comorbidities (gliclazide, cilazapril, atorvastatin, and acetylsalicylic acid) were studied for their interactions with bovine serum albumin—native and glycated. Two different spectroscopic methods, fluorescence quenching and circular dichroism, were utilized to elucidate the binding interactions of the investigational drugs. The glycation process was induced in BSA by glucose and was confirmed by the presence of advanced glycosylation end products (AGEs). The interaction between albumin and gliclazide, with the presence of another drug, was confirmed by calculation of association constants (0.11–1.07 × 104 M−1). The nature of changes in the secondary structure of a protein depends on the drug used and the degree of glycation. Therefore, these interactions may have an influence on pharmacokinetic parameters.

In Vitro Investigation of Binding Interactions between Albumin–Gliclazide Model and Typical Hypotensive Drugs

“…Figure 4 A shows the CD bands of BSA at 208 and 222 nm, which are characteristic of the α-helix structure of the protein. Both of these values contributed to the n → π* transition in the peptide bond of the α-helix [ 45 , 46 ]. The UV–CD spectra of the binary systems (BSA–DRUG) shown in Figure 4 A indicate that the binding of the drugs induced a negligible perturbation in the BSA secondary structure.…”

Investigation on the binding behavior of human α1-acid glycoprotein with Janus Kinase inhibitor baricitinib: Multi-spectroscopic and molecular simulation methodologies

“…Therefore, the study on the binding between drugs and plasma proteins is helpful to better understand the transport process of drugs in vivo . Recently, the quantity of publications has significantly increased focusing on the interaction of drugs with serum albumin [ [3] , [4] , [5] , [6] , [7] , [8] ]. In addition, the study of the interaction between drugs and small molecular ligands such as DNA has also aroused people's interest [ 9 , 10 ].…”