How functional genomics will impact fruit fly pest control: the example of the Mediterranean fruit fly, Ceratitis capitata

Scolari, Francesca; Gomulski, Ludvik M.; Gabrieli, Paolo; Manni, Mosè; Savini, Grazia; Gasperi, Giuliano; Malacrida, Anna R.

doi:10.1186/1471-2156-15-s2-s11

Cited by 13 publications

(9 citation statements)

References 68 publications

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“…Particularly, the medfly is a cosmopolitan pest and due to its great importance in the cultivation and export of more than 260 fruits and vegetables12, it has turned out to be a model organism in the Tephritidae family of insects and beyond, for studies ranging from classical genetics to genomics5474849505152, as well as area-wide control practices5354. The olive fly, on the other hand, is a strictly monophagous cousin of the medfly, of particular interest in the olive producing areas of the world4.…”

Section: Discussionmentioning

confidence: 99%

Housekeeping in Tephritid insects: the best gene choice for expression analyses in the medfly and the olive fly

Sagri

Koskinioti

Gregoriou

et al. 2017

Sci Rep

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Real-time quantitative-PCR has been a priceless tool for gene expression analyses. The reaction, however, needs proper normalization with the use of housekeeping genes (HKGs), whose expression remains stable throughout the experimental conditions. Often, the combination of several genes is required for accurate normalization. Most importantly, there are no universal HKGs which can be used since their expression varies among different organisms, tissues or experimental conditions. In the present study, nine common HKGs (RPL19, tbp, ubx, GAPDH, α-TUB, β-TUB, 14-3-3zeta, RPE and actin3) are evaluated in thirteen different body parts, developmental stages and reproductive and olfactory tissues of two insects of agricultural importance, the medfly and the olive fly. Three software programs based on different algorithms were used (geNorm, NormFinder and BestKeeper) and gave different ranking of HKG stabilities. This confirms once again that the stability of common HKGs should not be taken for granted and demonstrates the caution that is needed in the choice of the appropriate HKGs. Finally, by estimating the average of a standard score of the stability values resulted by the three programs we were able to provide a useful consensus key for the choice of the best HKG combination in various tissues of the two insects.

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Section: Discussionmentioning

confidence: 99%

Housekeeping in Tephritid insects: the best gene choice for expression analyses in the medfly and the olive fly

Sagri

Koskinioti

Gregoriou

et al. 2017

Sci Rep

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“…Starting from the model organism D. melanogaster 27 and extending to other insect species such as beetle 28 and mosquito species 29 among others, this approach has also been used to identify a wide range of genes involved in the specific area of chemosensory perception. Recently, this approach has also been used to identify and characterize genes in some species of the Tephritidae or true fruit flies that are major agricultural pests, including the Mediterranean fruit fly ( C. capitata ) 20 30 and the oriental fruit fly ( B. dorsalis ) 19 31 .…”

Section: Discussionmentioning

confidence: 99%

Identification and preliminary characterization of chemosensory perception-associated proteins in the melon fly Bactrocera cucurbitae using RNA-seq

Elfékih

Chen

Hsu

et al. 2016

Sci Rep

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An investigation into proteins involved in chemosensory perception in the melon fly, Bactrocera cucurbitae (Diptera: Tephritidae) is described here using a newly generated transcriptome dataset. The melon fly is a major agricultural pest, widely distributed in the Asia-Pacific region and some parts of Africa. For this study, a transcriptome dataset was generated using RNA extracted from 4-day-old adult specimens of the melon fly. The dataset was assembled and annotated via Gene Ontology (GO) analysis. Based on this and similarity searches to data from other species, a number of protein sequences putatively involved in chemosensory reception were identified and characterized in the melon fly. This included the highly conserved “Orco” along with a number of other less conserved odorant binding protein sequences. In addition, several sequences representing putative ionotropic and gustatory receptors were also identified. This study provides a foundation for future functional studies of chemosensory proteins in the melon fly and for making more detailed comparisons to other species. In the long term, this will ultimately help in the development of improved tools for pest management.

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“…Within the family of Tephritidae, several other insect members have been described to some detail such as Ceratitis capitata (the Mediterranean fruit fly) (Scolari et al, 2014) and B. oleae (the olive fly; Mavragani-Tsipidou, 2002). Common to this family is that all three fly species represent the most destructive of fruit pests.…”

Section: W Suksuwan Et Almentioning

confidence: 99%

Segmentation gene expression patterns in Bactrocera dorsalis and related insects: regulation and shape of blastoderm and larval cuticle

Suksuwan

Cai²,

Ngernsiri

et al. 2017

Int. J. Dev. Biol.

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The oriental fruit fly, Bactrocera dorsalis, is regarded as a severe pest of fruit production in Asia. Despite its economic importance, only limited information regarding the molecular and developmental biology of this insect is known to date. We provide a detailed analysis of B. dorsalis embryology, as well as the expression patterns of a number of segmentation genes known to act during patterning of Drosophila and compare these to the patterns of other insect families. An anterior shift of the expression of gap genes was detected when compared to Drosophila. This shift was largely restored during the step where the gap genes control expression of the pair-rule genes. We analyzed and compared the shapes of the embryos of insects of different families, B. dorsalis and the blow fly Lucilia sericata with that of the well-characterized Drosophila melanogaster. We found distinct shapes as well as differences in the ratios of the length of the anterior-posterior axis and the dorsal-ventral axis. These features were integrated into a profile of how the expression patterns of the gap gene Krüppel and the pair-rule gene even-skipped were observed along the A-P axis in three insects families. Since significant differences were observed, we discuss how Krüppel controls the even-skipped stripes. Furthermore, we discuss how the position and angles of the segmentation gene stripes differed from other insects. Finally, we analyzed the outcome of the expression patterns of the late acting segment polarity genes in relation to the anlagen of the naked-cuticle and denticle belt area of the B. dorsalis larva.

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How functional genomics will impact fruit fly pest control: the example of the Mediterranean fruit fly, Ceratitis capitata

Cited by 13 publications

References 68 publications

Housekeeping in Tephritid insects: the best gene choice for expression analyses in the medfly and the olive fly

Housekeeping in Tephritid insects: the best gene choice for expression analyses in the medfly and the olive fly

Identification and preliminary characterization of chemosensory perception-associated proteins in the melon fly Bactrocera cucurbitae using RNA-seq

Segmentation gene expression patterns in Bactrocera dorsalis and related insects: regulation and shape of blastoderm and larval cuticle

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