How gene expression in fast‐proliferating cells keeps pace

Martinho, Rui Gonçalo; Guilgur, Leonardo G.; Prudêncio, Pedro

doi:10.1002/bies.201400195

Cited by 8 publications

(12 citation statements)

References 128 publications

(187 reference statements)

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“…We demonstrate that the fas locus (Nüsslein-Volhard et al, 1984), previously reported to encode the secreted immunoglobulin domain protein CG17716 (Lekven et al, 1998), in fact encodes the NTC/Prp19C subunit CG6197 also known as fand (Guilgur et al, 2014). Second, we show that loss of zygotic fas function results in widespread perturbation of splicing, consistent with previous work demonstrating an essential requirement of maternal CG6197/fand function for efficient splicing during early embryogenesis (Guilgur et al, 2014;Martinho et al, 2015). Abnormal transcript processing in fas mutants manifests predominantly in intron retention, accompanied by changes in transcript abundance.…”

Section: Discussionsupporting

confidence: 89%

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Faithful mRNA splicing depends on the Prp19 complex subunit faint sausage and is required for tracheal branching morphogenesis in Drosophila

et al. 2017

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Morphogenesis requires the dynamic regulation of gene expression, including transcription, mRNA maturation and translation. Dysfunction of the general mRNA splicing machinery can cause surprisingly specific cellular phenotypes, but the basis for these effects is not clear. Here, we show that the Drosophila faint sausage (fas) locus, which is implicated in epithelial morphogenesis and has previously been reported to encode a secreted immunoglobulin domain protein, in fact encodes a subunit of the spliceosomeactivating Prp19 complex, which is essential for efficient pre-mRNA splicing. Loss of zygotic fas function globally impairs the efficiency of splicing, and is associated with widespread retention of introns in mRNAs and dramatic changes in gene expression. Surprisingly, despite these general effects, zygotic fas mutants show specific defects in tracheal cell migration during mid-embryogenesis when maternally supplied splicing factors have declined. We propose that tracheal branching, which relies on dynamic changes in gene expression, is particularly sensitive for efficient spliceosome function. Our results reveal an entry point to study requirements of the splicing machinery during organogenesis and provide a better understanding of disease phenotypes associated with mutations in general splicing factors.

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Section: Discussionsupporting

confidence: 89%

“…Maternally provided Fas protein is essential for efficient mRNA splicing in the early embryo (Guilgur et al, 2014;Martinho et al, 2015), but zygotic functions of fas have not been addressed thus far. We found that Fas protein levels were strongly reduced in fas…”

Section: Zygotic Loss Of Fas Causes Widespread Intron Retentionmentioning

confidence: 99%

Faithful mRNA splicing depends on the Prp19 complex subunit faint sausage and is required for tracheal branching morphogenesis in Drosophila

et al. 2017

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“…Nix has a very short intron and encodes a small protein: this condition is expected considering its putative male-determining function and its very early zygotic expression [46]. In Drosophila , the majority of early zygotic expressed genes are intronless or have small introns and encode small proteins [58, 59]. Nix transcription in Ae.…”

Section: Discussionmentioning

confidence: 99%

The Nix locus on the male-specific homologue of chromosome 1 in Aedes albopictus is a strong candidate for a male-determining factor

et al. 2018

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BackgroundGlobal concern over the rapid expansion of the Asian tiger mosquito, Aedes albopictus, and its vector competence has highlighted an urgent need to improve currently available population control methods, like the Sterile Insect Technique. Knowledge of the sex determination cascade is a prerequisite for the development of early-stage sexing systems. To this end, we have characterised the putative sex determination gene, Nix, in this species. In Aedes species the chromosome complement consists of three pairs of chromosomes. The sex determination alleles are linked to the smallest homomorphic chromosome.ResultsWe identified the male-specific chromosome 1 of Ae. albopictus that carries the putative male-determining gene Nix. We have also characterised the complete genomic sequence of the Nix gene which is composed of two exons and a short intron. The gene displays different levels of intron retention during development. Comparison of DNA sequences covering most of the Nix gene from individuals across the species range revealed no polymorphism.ConclusionsOur characterisation of the Nix gene in Ae. albopictus represents an initial step in the analysis of the sex determination cascade in this species. We found evidence of intron retention (IR) in Nix. IR might play a role in regulating the expression of Nix during development. Our results provide the basis for the development of new genetic control strategies.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3215-8) contains supplementary material, which is available to authorized users.

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“…The cell cycle focus we have adopted in this review does not delve into the events that control the major increase in transcription that occurs at the MBT or the events governing the elimination of maternal functions [13, 14, 17, 80, 81]. We do however, expect that multiple levels of interactions will interconnect and coordinate these different events as the embryo undergoes its massive transformation and refocuses its efforts from producing lots of cells to directing the development fates of those cells.…”

Section: Alternative Proposal For Triggering the Mbtmentioning

confidence: 99%

Timing the Drosophila Mid-Blastula Transition: A Cell Cycle-Centered View

et al. 2016

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At the Mid-Blastula Transition (MBT), externally developing embryos refocus from increasing cell number to elaboration of the body plan. Studies in Drosophila reveal a sequence of changes in regulators of Cyclin:Cdk1 that increasingly restricts the activity of this cell cycle kinase to slow cell cycles during early embryogenesis. By reviewing these events, we provide an outline of the mechanisms slowing the cell cycle at and around the time of MBT. The perspectives developed should provide a guiding paradigm for the study of other MBT changes as the embryo transits from maternal control to a regulatory program centered on the expression of zygotic genes.

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How gene expression in fast‐proliferating cells keeps pace

Cited by 8 publications

References 128 publications

Faithful mRNA splicing depends on the Prp19 complex subunit faint sausage and is required for tracheal branching morphogenesis in Drosophila

Faithful mRNA splicing depends on the Prp19 complex subunit faint sausage and is required for tracheal branching morphogenesis in Drosophila

The Nix locus on the male-specific homologue of chromosome 1 in Aedes albopictus is a strong candidate for a male-determining factor

Timing the Drosophila Mid-Blastula Transition: A Cell Cycle-Centered View

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