How mitochondria import proteins from the cytoplasm

Synthesis and Assembly of Mitochondrial Proteins

“…The labeled mitochondrial proteins first appeared in a cytosolic pool and then in mitochondria. Similarly, in yeast, mitochondrial precursor proteins first appeared in a cytosolic pool and were then subsequently imported into mitochondria and converted to their mature forms (Reid and Schatz, 1982b;Schatz, 1979).…”

Transport into mitochondria and intramitochondrial sorting of the Fe/S protein of ubiquinol-cytochrome c reductase

“…First, completed polypeptide chains are released as precursors, most of which carry amino-terminal peptide extensions, from cytoplasmic ribosomes into precursor pools in the cytosol (Hallermayer et al, 1977;Schatz, 1979). Second, precursors are targeted to mitochondria by specific signals contained in the presequences (Hurt et al, 1984a(Hurt et al, , 1984bHorwich et al.…”

Mitochondrial protein import: Specific recognition and membrane translocation of preproteins

“…Import into mitochondria can occur in a post-translational manner in vitro (Korb & Neupert, 1978;Maccecchini, Rudin & Schatz, 1979) and in vivo (Hallermayer, Zimmerman & Neupert, 1977;Schatz, 1979;Wienhues et al, 1991), showing that transport is mechanistically independent of elongation. Therefore, polypeptide chain elongation cannot be the driving force for mitochondrial protein import.…”